Subtractive genomics approach towards the identification of novel therapeutic targets against human Bartonella bacilliformis
Bartonella bacilliformis, a causative agent of Bartonellosis or Carrión's disease, has become resistant to antibacterial agents. To suggest effective novel drug targets, a comprehensive in silico subtractive genomic approach was employed in the genome of B. bacilliformis subsp. ver097. Differen...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-01-01
|
| Series: | Informatics in Medicine Unlocked |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352914820303920 |
| _version_ | 1818883471803678720 |
|---|---|
| author | Md. Tahsin Khan Araf Mahmud Asif Iqbal Syeda Farjana Hoque Mahmudul Hasan |
| author_facet | Md. Tahsin Khan Araf Mahmud Asif Iqbal Syeda Farjana Hoque Mahmudul Hasan |
| author_sort | Md. Tahsin Khan |
| collection | DOAJ |
| description | Bartonella bacilliformis, a causative agent of Bartonellosis or Carrión's disease, has become resistant to antibacterial agents. To suggest effective novel drug targets, a comprehensive in silico subtractive genomic approach was employed in the genome of B. bacilliformis subsp. ver097. Different bioinformatics tools and online servers were utilized to determine human homologous proteins of the pathogen, as well as proteins associated with common metabolic pathways of pathogen and host. Only seven proteins were associated in pathogen specific pathways, which were further screened to determine drug or vaccine targeted membrane proteins. ‘ABC transporter permease’ and ‘Flagellar biosynthesis protein FlhA’ were found to be novel drug targets which showed the highest level of antigenicity. Moreover, ‘Flagellar biosynthesis protein FlhA’ exhibited broad spectrum conservancy with other Bartonella bacilliformis strains. Consequently, it might be used for development of novel drugs and therapeutic components for the successful treatment of infections caused by Bartonella bacilliformis. |
| first_indexed | 2024-12-19T15:34:11Z |
| format | Article |
| id | doaj.art-970e1b37e33147b785a8fac943d05b8d |
| institution | Directory Open Access Journal |
| issn | 2352-9148 |
| language | English |
| last_indexed | 2024-12-19T15:34:11Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Informatics in Medicine Unlocked |
| spelling | doaj.art-970e1b37e33147b785a8fac943d05b8d2022-12-21T20:15:39ZengElsevierInformatics in Medicine Unlocked2352-91482020-01-0120100385Subtractive genomics approach towards the identification of novel therapeutic targets against human Bartonella bacilliformisMd. Tahsin Khan0Araf Mahmud1Asif Iqbal2Syeda Farjana Hoque3Mahmudul Hasan4Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, 3114, BangladeshDepartment of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, 3114, BangladeshDepartment of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, 3114, BangladeshDepartment of Pharmaceuticals and Industrial Biotechnology, Sylhet Agricultural University, Sylhet, 3100, BangladeshDepartment of Pharmaceuticals and Industrial Biotechnology, Sylhet Agricultural University, Sylhet, 3100, Bangladesh; Corresponding author.Bartonella bacilliformis, a causative agent of Bartonellosis or Carrión's disease, has become resistant to antibacterial agents. To suggest effective novel drug targets, a comprehensive in silico subtractive genomic approach was employed in the genome of B. bacilliformis subsp. ver097. Different bioinformatics tools and online servers were utilized to determine human homologous proteins of the pathogen, as well as proteins associated with common metabolic pathways of pathogen and host. Only seven proteins were associated in pathogen specific pathways, which were further screened to determine drug or vaccine targeted membrane proteins. ‘ABC transporter permease’ and ‘Flagellar biosynthesis protein FlhA’ were found to be novel drug targets which showed the highest level of antigenicity. Moreover, ‘Flagellar biosynthesis protein FlhA’ exhibited broad spectrum conservancy with other Bartonella bacilliformis strains. Consequently, it might be used for development of novel drugs and therapeutic components for the successful treatment of infections caused by Bartonella bacilliformis.http://www.sciencedirect.com/science/article/pii/S2352914820303920Bartonella bacilliformisCarrión's diseaseSubtractive genomicsDrug targetVaccine |
| spellingShingle | Md. Tahsin Khan Araf Mahmud Asif Iqbal Syeda Farjana Hoque Mahmudul Hasan Subtractive genomics approach towards the identification of novel therapeutic targets against human Bartonella bacilliformis Informatics in Medicine Unlocked Bartonella bacilliformis Carrión's disease Subtractive genomics Drug target Vaccine |
| title | Subtractive genomics approach towards the identification of novel therapeutic targets against human Bartonella bacilliformis |
| title_full | Subtractive genomics approach towards the identification of novel therapeutic targets against human Bartonella bacilliformis |
| title_fullStr | Subtractive genomics approach towards the identification of novel therapeutic targets against human Bartonella bacilliformis |
| title_full_unstemmed | Subtractive genomics approach towards the identification of novel therapeutic targets against human Bartonella bacilliformis |
| title_short | Subtractive genomics approach towards the identification of novel therapeutic targets against human Bartonella bacilliformis |
| title_sort | subtractive genomics approach towards the identification of novel therapeutic targets against human bartonella bacilliformis |
| topic | Bartonella bacilliformis Carrión's disease Subtractive genomics Drug target Vaccine |
| url | http://www.sciencedirect.com/science/article/pii/S2352914820303920 |
| work_keys_str_mv | AT mdtahsinkhan subtractivegenomicsapproachtowardstheidentificationofnoveltherapeutictargetsagainsthumanbartonellabacilliformis AT arafmahmud subtractivegenomicsapproachtowardstheidentificationofnoveltherapeutictargetsagainsthumanbartonellabacilliformis AT asifiqbal subtractivegenomicsapproachtowardstheidentificationofnoveltherapeutictargetsagainsthumanbartonellabacilliformis AT syedafarjanahoque subtractivegenomicsapproachtowardstheidentificationofnoveltherapeutictargetsagainsthumanbartonellabacilliformis AT mahmudulhasan subtractivegenomicsapproachtowardstheidentificationofnoveltherapeutictargetsagainsthumanbartonellabacilliformis |