Whole genome sequencing of <i>Acinetobacter baumannii</i> strains isolated from hospital patients in the northern territories of the Tyumen region
Introduction. is to The analysis of the genetic relatedness of isolates aiming to find the source of infection is an important task of nosocomial infection control. The most common causative agent of healthcare-associated infections is Acinetobacter baumannii.Objective. To evaluate the results of w...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2022-07-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.elpub.ru/jour/article/view/1262 |
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| author | L. V. Kataeva O. N. Kolotova T. F. Stepanova A. A. Kislichkina L. A. Shishkina T. N. Mukhina |
| author_facet | L. V. Kataeva O. N. Kolotova T. F. Stepanova A. A. Kislichkina L. A. Shishkina T. N. Mukhina |
| author_sort | L. V. Kataeva |
| collection | DOAJ |
| description | Introduction. is to The analysis of the genetic relatedness of isolates aiming to find the source of infection is an important task of nosocomial infection control. The most common causative agent of healthcare-associated infections is Acinetobacter baumannii.Objective. To evaluate the results of whole genome sequencing of A. baumannii bacteria isolated from clinical samples of patients undergoing inpatient treatment in the northern territories of the Tyumen region.Materials and methods. Nine isolates of A. baumannii from the clinical material of patients were studied. Bacterial cultures were identified by mass spectrometry. Whole genome sequencing, multilocus sequence typing and search for markers of antibiotic resistance were performed.Results. The studied strains belonged to sequence types ST2 and ST187, and to the international clonal complex CC2. All A. baumannii isolates were found to have beta-lactamase genes, as well as genes for resistance to aminoglycosides, to the MLS group of antibiotics, and to tetracyclines. The presence of a cluster of genes associated with virulence was detected: those responsible for the synthesis of acinetobactin and iron binding, surface antigen 1 and porin.Conclusion. Based on data of a single nucleotide polymorphism (SNP) analysis, A. baumannii isolates from the clinical material of patients of healthcare institution #1 belong mainly to one bacterial strain. Isolates of A. baumannii from the clinical material of patients of healthcare institution #2 are closely related. The ability to distinguish clinical isolates of A. baumannii at the level of several SNPs per genome will improve the identification of the source of infection, and whole genome sequencing data can contribute to the rational prescription of antibiotic therapy and the correction of disinfection and antiseptic measures. |
| first_indexed | 2024-04-13T02:42:16Z |
| format | Article |
| id | doaj.art-970e21b0f59a4b1c9310339eabfcb7a8 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-04-13T02:42:16Z |
| publishDate | 2022-07-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-970e21b0f59a4b1c9310339eabfcb7a82022-12-22T03:06:10ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132022-07-0199334335210.36233/0372-9311-231816Whole genome sequencing of <i>Acinetobacter baumannii</i> strains isolated from hospital patients in the northern territories of the Tyumen regionL. V. Kataeva0O. N. Kolotova1T. F. Stepanova2A. A. Kislichkina3L. A. Shishkina4T. N. Mukhina5Tyumen Region Infection Pathology Research InstituteTyumen Region Infection Pathology Research InstituteTyumen Region Infection Pathology Research InstituteState Research Center for Applied Microbiology and BiotechnologyState Research Center for Applied Microbiology and BiotechnologyState Research Center for Applied Microbiology and BiotechnologyIntroduction. is to The analysis of the genetic relatedness of isolates aiming to find the source of infection is an important task of nosocomial infection control. The most common causative agent of healthcare-associated infections is Acinetobacter baumannii.Objective. To evaluate the results of whole genome sequencing of A. baumannii bacteria isolated from clinical samples of patients undergoing inpatient treatment in the northern territories of the Tyumen region.Materials and methods. Nine isolates of A. baumannii from the clinical material of patients were studied. Bacterial cultures were identified by mass spectrometry. Whole genome sequencing, multilocus sequence typing and search for markers of antibiotic resistance were performed.Results. The studied strains belonged to sequence types ST2 and ST187, and to the international clonal complex CC2. All A. baumannii isolates were found to have beta-lactamase genes, as well as genes for resistance to aminoglycosides, to the MLS group of antibiotics, and to tetracyclines. The presence of a cluster of genes associated with virulence was detected: those responsible for the synthesis of acinetobactin and iron binding, surface antigen 1 and porin.Conclusion. Based on data of a single nucleotide polymorphism (SNP) analysis, A. baumannii isolates from the clinical material of patients of healthcare institution #1 belong mainly to one bacterial strain. Isolates of A. baumannii from the clinical material of patients of healthcare institution #2 are closely related. The ability to distinguish clinical isolates of A. baumannii at the level of several SNPs per genome will improve the identification of the source of infection, and whole genome sequencing data can contribute to the rational prescription of antibiotic therapy and the correction of disinfection and antiseptic measures.https://microbiol.elpub.ru/jour/article/view/1262acinetobacter baumanniiclinical strainswhole genome sequencingresistance genessequence types |
| spellingShingle | L. V. Kataeva O. N. Kolotova T. F. Stepanova A. A. Kislichkina L. A. Shishkina T. N. Mukhina Whole genome sequencing of <i>Acinetobacter baumannii</i> strains isolated from hospital patients in the northern territories of the Tyumen region Журнал микробиологии, эпидемиологии и иммунобиологии acinetobacter baumannii clinical strains whole genome sequencing resistance genes sequence types |
| title | Whole genome sequencing of <i>Acinetobacter baumannii</i> strains isolated from hospital patients in the northern territories of the Tyumen region |
| title_full | Whole genome sequencing of <i>Acinetobacter baumannii</i> strains isolated from hospital patients in the northern territories of the Tyumen region |
| title_fullStr | Whole genome sequencing of <i>Acinetobacter baumannii</i> strains isolated from hospital patients in the northern territories of the Tyumen region |
| title_full_unstemmed | Whole genome sequencing of <i>Acinetobacter baumannii</i> strains isolated from hospital patients in the northern territories of the Tyumen region |
| title_short | Whole genome sequencing of <i>Acinetobacter baumannii</i> strains isolated from hospital patients in the northern territories of the Tyumen region |
| title_sort | whole genome sequencing of i acinetobacter baumannii i strains isolated from hospital patients in the northern territories of the tyumen region |
| topic | acinetobacter baumannii clinical strains whole genome sequencing resistance genes sequence types |
| url | https://microbiol.elpub.ru/jour/article/view/1262 |
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