Phytochemical Characterization, Anti-Oxidant, Anti-Enzymatic and Cytotoxic Effects of <i>Artemisia verlotiorum</i> Lamotte Extracts: A New Source of Bioactive Agents
<i>Artemisia verlotiorum</i> Lamotte is recognized medicinally given its long-standing ethnopharmacological uses in different parts of the world. Nonetheless, the pharmacological properties of the leaves of the plant have been poorly studied by the scientific community. Hence, this study...
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MDPI AG
2022-09-01
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| author | Shanoo Suroowan Eulogio Jose Llorent-Martínez Gokhan Zengin Stefano Dall’Acqua Stefania Sut Kalaivani Buskaran Sharida Fakurazi Mohamad Fawzi Mahomoodally |
| author_facet | Shanoo Suroowan Eulogio Jose Llorent-Martínez Gokhan Zengin Stefano Dall’Acqua Stefania Sut Kalaivani Buskaran Sharida Fakurazi Mohamad Fawzi Mahomoodally |
| author_sort | Shanoo Suroowan |
| collection | DOAJ |
| description | <i>Artemisia verlotiorum</i> Lamotte is recognized medicinally given its long-standing ethnopharmacological uses in different parts of the world. Nonetheless, the pharmacological properties of the leaves of the plant have been poorly studied by the scientific community. Hence, this study aimed to decipher the phytochemicals; quantify through HPLC-ESI-MS analysis the plant’s biosynthesis; and evaluate the antioxidant, anti-tyrosinase, amylase, glucosidase, cholinesterase, and cytotoxicity potential on normal (NIH 3T3) and human liver and human colon cancer (HepG2 and HT 29) cell lines of this plant species. The aqueous extract contained the highest content of phenolics and phenolic acid, methanol extracted the most flavonoid, and the most flavonol was extracted by ethyl acetate. The one-way ANOVA results demonstrated that all results obtained were statistically significant at <i>p</i> < 0.05. A total of 25 phytoconstituents were identified from the different extracts, with phenolic acids and flavonoids being the main metabolites. The highest antioxidant potential was recorded for the aqueous extract. The best anti-tyrosinase extract was the methanolic extract. The ethyl acetate extract of <i>A. verlotiorum</i> had the highest flavonol content and hence was most active against the cholinesterase enzymes. The ethyl acetate extract was the best α-glucosidase and α-amylase inhibitor. The samples of <i>Artemisia verlotiorum</i> Lamotte in both aqueous and methanolic extracts were found to be non-toxic after 48 h against NIH 3T3 cells. In HepG2 cells, the methanolic extract was nontoxic up to 125 µg/mL, and an IC<sub>50</sub> value of 722.39 µg/mL was recorded. The IC<sub>50</sub> value exhibited in methanolic extraction of <i>A. verlotiorum</i> was 792.91 µg/mL in HT29 cells. Methanolic extraction is capable of inducing cell cytotoxicity in human hepatocellular carcinoma without damaging normal cells. Hence, <i>A. verlotiorum</i> can be recommended for further evaluation of its phytochemical and medicinal properties. |
| first_indexed | 2024-03-09T23:02:09Z |
| format | Article |
| id | doaj.art-970e84ff70174c4789e296be663db5dd |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:02:09Z |
| publishDate | 2022-09-01 |
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| series | Molecules |
| spelling | doaj.art-970e84ff70174c4789e296be663db5dd2023-11-23T18:00:34ZengMDPI AGMolecules1420-30492022-09-012718588610.3390/molecules27185886Phytochemical Characterization, Anti-Oxidant, Anti-Enzymatic and Cytotoxic Effects of <i>Artemisia verlotiorum</i> Lamotte Extracts: A New Source of Bioactive AgentsShanoo Suroowan0Eulogio Jose Llorent-Martínez1Gokhan Zengin2Stefano Dall’Acqua3Stefania Sut4Kalaivani Buskaran5Sharida Fakurazi6Mohamad Fawzi Mahomoodally7Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Réduit, Moka 80837, MauritiusDepartment of Physical and Analytical Chemistry, University of Jaén, Campus Las Lagunillas S/N, E-23071 Jaén, SpainDepartment of Biology, Science Faculty, Selcuk University, Konya 42130, TurkeyDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, ItalyDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, ItalyLaboratory of Natural Medicine and Product Research, Institute of Bioscience, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor Darul Ehsan, MalaysiaLaboratory of Natural Medicine and Product Research, Institute of Bioscience, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor Darul Ehsan, MalaysiaDepartment of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Réduit, Moka 80837, Mauritius<i>Artemisia verlotiorum</i> Lamotte is recognized medicinally given its long-standing ethnopharmacological uses in different parts of the world. Nonetheless, the pharmacological properties of the leaves of the plant have been poorly studied by the scientific community. Hence, this study aimed to decipher the phytochemicals; quantify through HPLC-ESI-MS analysis the plant’s biosynthesis; and evaluate the antioxidant, anti-tyrosinase, amylase, glucosidase, cholinesterase, and cytotoxicity potential on normal (NIH 3T3) and human liver and human colon cancer (HepG2 and HT 29) cell lines of this plant species. The aqueous extract contained the highest content of phenolics and phenolic acid, methanol extracted the most flavonoid, and the most flavonol was extracted by ethyl acetate. The one-way ANOVA results demonstrated that all results obtained were statistically significant at <i>p</i> < 0.05. A total of 25 phytoconstituents were identified from the different extracts, with phenolic acids and flavonoids being the main metabolites. The highest antioxidant potential was recorded for the aqueous extract. The best anti-tyrosinase extract was the methanolic extract. The ethyl acetate extract of <i>A. verlotiorum</i> had the highest flavonol content and hence was most active against the cholinesterase enzymes. The ethyl acetate extract was the best α-glucosidase and α-amylase inhibitor. The samples of <i>Artemisia verlotiorum</i> Lamotte in both aqueous and methanolic extracts were found to be non-toxic after 48 h against NIH 3T3 cells. In HepG2 cells, the methanolic extract was nontoxic up to 125 µg/mL, and an IC<sub>50</sub> value of 722.39 µg/mL was recorded. The IC<sub>50</sub> value exhibited in methanolic extraction of <i>A. verlotiorum</i> was 792.91 µg/mL in HT29 cells. Methanolic extraction is capable of inducing cell cytotoxicity in human hepatocellular carcinoma without damaging normal cells. Hence, <i>A. verlotiorum</i> can be recommended for further evaluation of its phytochemical and medicinal properties.https://www.mdpi.com/1420-3049/27/18/5886<i>Artemisia verlotiorum</i>cytotoxicityamylasetyrosinasephyto-pharmaceutics |
| spellingShingle | Shanoo Suroowan Eulogio Jose Llorent-Martínez Gokhan Zengin Stefano Dall’Acqua Stefania Sut Kalaivani Buskaran Sharida Fakurazi Mohamad Fawzi Mahomoodally Phytochemical Characterization, Anti-Oxidant, Anti-Enzymatic and Cytotoxic Effects of <i>Artemisia verlotiorum</i> Lamotte Extracts: A New Source of Bioactive Agents Molecules <i>Artemisia verlotiorum</i> cytotoxicity amylase tyrosinase phyto-pharmaceutics |
| title | Phytochemical Characterization, Anti-Oxidant, Anti-Enzymatic and Cytotoxic Effects of <i>Artemisia verlotiorum</i> Lamotte Extracts: A New Source of Bioactive Agents |
| title_full | Phytochemical Characterization, Anti-Oxidant, Anti-Enzymatic and Cytotoxic Effects of <i>Artemisia verlotiorum</i> Lamotte Extracts: A New Source of Bioactive Agents |
| title_fullStr | Phytochemical Characterization, Anti-Oxidant, Anti-Enzymatic and Cytotoxic Effects of <i>Artemisia verlotiorum</i> Lamotte Extracts: A New Source of Bioactive Agents |
| title_full_unstemmed | Phytochemical Characterization, Anti-Oxidant, Anti-Enzymatic and Cytotoxic Effects of <i>Artemisia verlotiorum</i> Lamotte Extracts: A New Source of Bioactive Agents |
| title_short | Phytochemical Characterization, Anti-Oxidant, Anti-Enzymatic and Cytotoxic Effects of <i>Artemisia verlotiorum</i> Lamotte Extracts: A New Source of Bioactive Agents |
| title_sort | phytochemical characterization anti oxidant anti enzymatic and cytotoxic effects of i artemisia verlotiorum i lamotte extracts a new source of bioactive agents |
| topic | <i>Artemisia verlotiorum</i> cytotoxicity amylase tyrosinase phyto-pharmaceutics |
| url | https://www.mdpi.com/1420-3049/27/18/5886 |
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