Hybrid Nanobeads for Oral Indomethacin Delivery
The oral administration of the anti-inflammatory indomethacin (INDO) causes severe gastrointestinal side effects, which are intensified in chronic inflammatory conditions when a continuous treatment is mandatory. The development of hybrid delivery systems associates the benefits of different (nano)...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/3/583 |
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| author | Flávia Monique Rocha Bonetti Eneida de Paula Belchiolina Beatriz Fonseca Gabriela Ribeiro da Silva Leandro Santana Soares da Silva Ludmilla David de Moura Márcia Cristina Breitkreitz Gustavo Henrique Rodrigues da Silva Lígia Nunes de Morais Ribeiro |
| author_facet | Flávia Monique Rocha Bonetti Eneida de Paula Belchiolina Beatriz Fonseca Gabriela Ribeiro da Silva Leandro Santana Soares da Silva Ludmilla David de Moura Márcia Cristina Breitkreitz Gustavo Henrique Rodrigues da Silva Lígia Nunes de Morais Ribeiro |
| author_sort | Flávia Monique Rocha Bonetti |
| collection | DOAJ |
| description | The oral administration of the anti-inflammatory indomethacin (INDO) causes severe gastrointestinal side effects, which are intensified in chronic inflammatory conditions when a continuous treatment is mandatory. The development of hybrid delivery systems associates the benefits of different (nano) carriers in a single system, designed to improve the efficacy and/or minimize the toxicity of drugs. This work describes the preparation of hybrid nanobeads composed of nanostructured lipid carriers (NLC) loading INDO (2%; <i>w</i>/<i>v</i>) and chitosan, coated by xanthan. NLC formulations were monitored in a long-term stability study (25 °C). After one year, they showed suitable physicochemical properties (size < 250 nm, polydispersity < 0.2, zeta potential of −30 mV and spherical morphology) and an INDO encapsulation efficiency of 99%. The hybrid (lipid-biopolymers) nanobeads exhibited excellent compatibility between the biomaterials, as revealed by structural and thermodynamic properties, monodisperse size distribution, desirable in vitro water uptake and prolonged in vitro INDO release (26 h). The in vivo safety of hybrid nanobeads was confirmed by the chicken embryo (CE) toxicity test, considering the embryos viability, weights of CE and annexes and changes in the biochemical markers. The results point out a safe gastro-resistant pharmaceutical form for further efficacy assays. |
| first_indexed | 2024-03-09T12:58:59Z |
| format | Article |
| id | doaj.art-970fd11fd767432cac8cdb9f6244d57b |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T12:58:59Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-970fd11fd767432cac8cdb9f6244d57b2023-11-30T21:56:59ZengMDPI AGPharmaceutics1999-49232022-03-0114358310.3390/pharmaceutics14030583Hybrid Nanobeads for Oral Indomethacin DeliveryFlávia Monique Rocha Bonetti0Eneida de Paula1Belchiolina Beatriz Fonseca2Gabriela Ribeiro da Silva3Leandro Santana Soares da Silva4Ludmilla David de Moura5Márcia Cristina Breitkreitz6Gustavo Henrique Rodrigues da Silva7Lígia Nunes de Morais Ribeiro8Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas—UNICAMP, Campinas 13083-862, SP, BrazilDepartment of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas—UNICAMP, Campinas 13083-862, SP, BrazilSchool of Veterinary Medicine, Federal University of Uberlândia—UFU, Uberlândia 38410-337, MG, BrazilSchool of Veterinary Medicine, Federal University of Uberlândia—UFU, Uberlândia 38410-337, MG, BrazilSchool of Veterinary Medicine, Federal University of Uberlândia—UFU, Uberlândia 38410-337, MG, BrazilDepartment of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas—UNICAMP, Campinas 13083-862, SP, BrazilDepartment of Analytical Chemistry, Institute of Chemistry, University of Campinas—UNICAMP, Campinas 13083-970, SP, BrazilDepartment of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas—UNICAMP, Campinas 13083-862, SP, BrazilDepartment of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas—UNICAMP, Campinas 13083-862, SP, BrazilThe oral administration of the anti-inflammatory indomethacin (INDO) causes severe gastrointestinal side effects, which are intensified in chronic inflammatory conditions when a continuous treatment is mandatory. The development of hybrid delivery systems associates the benefits of different (nano) carriers in a single system, designed to improve the efficacy and/or minimize the toxicity of drugs. This work describes the preparation of hybrid nanobeads composed of nanostructured lipid carriers (NLC) loading INDO (2%; <i>w</i>/<i>v</i>) and chitosan, coated by xanthan. NLC formulations were monitored in a long-term stability study (25 °C). After one year, they showed suitable physicochemical properties (size < 250 nm, polydispersity < 0.2, zeta potential of −30 mV and spherical morphology) and an INDO encapsulation efficiency of 99%. The hybrid (lipid-biopolymers) nanobeads exhibited excellent compatibility between the biomaterials, as revealed by structural and thermodynamic properties, monodisperse size distribution, desirable in vitro water uptake and prolonged in vitro INDO release (26 h). The in vivo safety of hybrid nanobeads was confirmed by the chicken embryo (CE) toxicity test, considering the embryos viability, weights of CE and annexes and changes in the biochemical markers. The results point out a safe gastro-resistant pharmaceutical form for further efficacy assays.https://www.mdpi.com/1999-4923/14/3/583NLChybrid nanobeadbiopolymersanti-inflammatorychicken embryo model |
| spellingShingle | Flávia Monique Rocha Bonetti Eneida de Paula Belchiolina Beatriz Fonseca Gabriela Ribeiro da Silva Leandro Santana Soares da Silva Ludmilla David de Moura Márcia Cristina Breitkreitz Gustavo Henrique Rodrigues da Silva Lígia Nunes de Morais Ribeiro Hybrid Nanobeads for Oral Indomethacin Delivery Pharmaceutics NLC hybrid nanobead biopolymers anti-inflammatory chicken embryo model |
| title | Hybrid Nanobeads for Oral Indomethacin Delivery |
| title_full | Hybrid Nanobeads for Oral Indomethacin Delivery |
| title_fullStr | Hybrid Nanobeads for Oral Indomethacin Delivery |
| title_full_unstemmed | Hybrid Nanobeads for Oral Indomethacin Delivery |
| title_short | Hybrid Nanobeads for Oral Indomethacin Delivery |
| title_sort | hybrid nanobeads for oral indomethacin delivery |
| topic | NLC hybrid nanobead biopolymers anti-inflammatory chicken embryo model |
| url | https://www.mdpi.com/1999-4923/14/3/583 |
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