Composition of norfloxacin-resistant bacteria and isolation of norfloxacin-degrading bacteria in subtropical aquaculture ponds in China
To analyze the composition of norfloxacin-resistant bacteria and norfloxacin-degrading bacteria in pond water and sediment in subtropical China, the composition of antibiotic resistant bacteria in pond water and sediment enriched with norfloxacin-containing medium was analyzed by high-throughput seq...
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| Format: | Article |
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Polish Academy of Sciences
2022-12-01
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| Series: | Archives of Environmental Protection |
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| Online Access: | https://journals.pan.pl/Content/125246/PDF/Archives%20vol48no4pp94_101.pdf |
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| author | Lutian Mao Lifen Chen Xirui Wang Zhongbao Xu Hui Ouyang Biyou Huang Libin Zhou |
| author_facet | Lutian Mao Lifen Chen Xirui Wang Zhongbao Xu Hui Ouyang Biyou Huang Libin Zhou |
| author_sort | Lutian Mao |
| collection | DOAJ |
| description | To analyze the composition of norfloxacin-resistant bacteria and norfloxacin-degrading bacteria in pond water and sediment in subtropical China, the composition of antibiotic resistant bacteria in pond water and sediment enriched with norfloxacin-containing medium was analyzed by high-throughput sequencing. Sediment and water samples were collected from 3 fish ponds in subtropical China, and domesticated with norfloxacin, subsequently norfloxacin-resistant bacteria through high-throughput sequencing of 16S rDNA, and isolated norfloxacin- degrading bacteria. Our results showed that the pond sediment and water contain a variety of norfloxacin-resistant bacteria, mainly from Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, and Chloroflexi. Moreover, we isolated two norfloxacin-degrading bacteria (NorXu-2 and NorXu-3). The norfloxacin-degrading rate by NorXu-2 and NorXu-3 in the culture mediums with 200 μg/mL was the highest, which was up to 49.71% and 35.79%,respectively. When the norfloxacin concentration was 200 μg/mL, NorXu-2 and NorXu-3 had the best norfloxacin-degrading effect at pH of 6, and the degradation rates were 53.64% and 45.54%, respectively. Moreover, NorXu-3 exhibited a good tolerance to high NaCl concentration. These results not only provided basic data for the follow-up study of the molecular mechanism of antimicrobial microbial degradation, but also provided potential norfloxacin degrading bacteria for norfloxacin removal and bioremediation in aquaculture environment. |
| first_indexed | 2024-03-09T14:11:01Z |
| format | Article |
| id | doaj.art-970ffef2284d4f20b1e59273a850ee69 |
| institution | Directory Open Access Journal |
| issn | 2083-4772 2083-4810 |
| language | English |
| last_indexed | 2024-03-09T14:11:01Z |
| publishDate | 2022-12-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Archives of Environmental Protection |
| spelling | doaj.art-970ffef2284d4f20b1e59273a850ee692023-11-29T10:42:31ZengPolish Academy of SciencesArchives of Environmental Protection2083-47722083-48102022-12-01vol. 48No 495101https://doi.org/10.24425/aep.2022.143712Composition of norfloxacin-resistant bacteria and isolation of norfloxacin-degrading bacteria in subtropical aquaculture ponds in ChinaLutian Mao0Lifen Chen1Xirui Wang2Zhongbao Xu3Hui Ouyang4Biyou Huang5Libin Zhou6Huizhou University, Huizhou City, ChinaHuizhou University, Huizhou City, ChinaHuizhou University, Huizhou City, ChinaHuizhou University, Huizhou City, ChinaHuizhou University, Huizhou City, ChinaHuizhou University, Huizhou City, ChinaHuizhou University, Huizhou City, ChinaTo analyze the composition of norfloxacin-resistant bacteria and norfloxacin-degrading bacteria in pond water and sediment in subtropical China, the composition of antibiotic resistant bacteria in pond water and sediment enriched with norfloxacin-containing medium was analyzed by high-throughput sequencing. Sediment and water samples were collected from 3 fish ponds in subtropical China, and domesticated with norfloxacin, subsequently norfloxacin-resistant bacteria through high-throughput sequencing of 16S rDNA, and isolated norfloxacin- degrading bacteria. Our results showed that the pond sediment and water contain a variety of norfloxacin-resistant bacteria, mainly from Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, and Chloroflexi. Moreover, we isolated two norfloxacin-degrading bacteria (NorXu-2 and NorXu-3). The norfloxacin-degrading rate by NorXu-2 and NorXu-3 in the culture mediums with 200 μg/mL was the highest, which was up to 49.71% and 35.79%,respectively. When the norfloxacin concentration was 200 μg/mL, NorXu-2 and NorXu-3 had the best norfloxacin-degrading effect at pH of 6, and the degradation rates were 53.64% and 45.54%, respectively. Moreover, NorXu-3 exhibited a good tolerance to high NaCl concentration. These results not only provided basic data for the follow-up study of the molecular mechanism of antimicrobial microbial degradation, but also provided potential norfloxacin degrading bacteria for norfloxacin removal and bioremediation in aquaculture environment.https://journals.pan.pl/Content/125246/PDF/Archives%20vol48no4pp94_101.pdfhigh-throughput sequencingnorfloxacinantibiotic resistant bacteriumpond culture |
| spellingShingle | Lutian Mao Lifen Chen Xirui Wang Zhongbao Xu Hui Ouyang Biyou Huang Libin Zhou Composition of norfloxacin-resistant bacteria and isolation of norfloxacin-degrading bacteria in subtropical aquaculture ponds in China Archives of Environmental Protection high-throughput sequencing norfloxacin antibiotic resistant bacterium pond culture |
| title | Composition of norfloxacin-resistant bacteria and isolation of norfloxacin-degrading bacteria in subtropical aquaculture ponds in China |
| title_full | Composition of norfloxacin-resistant bacteria and isolation of norfloxacin-degrading bacteria in subtropical aquaculture ponds in China |
| title_fullStr | Composition of norfloxacin-resistant bacteria and isolation of norfloxacin-degrading bacteria in subtropical aquaculture ponds in China |
| title_full_unstemmed | Composition of norfloxacin-resistant bacteria and isolation of norfloxacin-degrading bacteria in subtropical aquaculture ponds in China |
| title_short | Composition of norfloxacin-resistant bacteria and isolation of norfloxacin-degrading bacteria in subtropical aquaculture ponds in China |
| title_sort | composition of norfloxacin resistant bacteria and isolation of norfloxacin degrading bacteria in subtropical aquaculture ponds in china |
| topic | high-throughput sequencing norfloxacin antibiotic resistant bacterium pond culture |
| url | https://journals.pan.pl/Content/125246/PDF/Archives%20vol48no4pp94_101.pdf |
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