Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)
The fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demo...
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| Format: | Article |
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BMC
2013-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200012&lng=en&tlng=en |
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| author | Zhengquan Gao Chunxiao Meng Hongzheng Gao Xiaowen Zhang Dong Xu Yuanfeng Su Yuanyuan Wang Yuren Zhao Naihao Ye |
| author_facet | Zhengquan Gao Chunxiao Meng Hongzheng Gao Xiaowen Zhang Dong Xu Yuanfeng Su Yuanyuan Wang Yuren Zhao Naihao Ye |
| author_sort | Zhengquan Gao |
| collection | DOAJ |
| description | The fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demonstrated that both 25 and 50 mg/L EBR could increase astaxanthin productivity and the eight carotenogenic genes were up-regulated by EBR with different expression profiles. Moreover, EBR25 induction had a greater influence on the transcriptional expression of ipi-1, ipi-2, crtR-B, lyc and crtO (> 5- fold up-regulation) than on psy, pds, bkt; EBR50 treatment had a greater effect on the transcriptional expression of ipi-2, pds, lyc, crtR-B, bkt and crtO than on ipi-1 and psy. Furthermore, astaxanthin biosynthesis under EBR was up-regulated mainly by ipi1־ and psy at the post-transcriptional level, pds, lyc, crtR-B, bkt and crtO at the transcriptional level and ipi-2 at both levels. |
| first_indexed | 2024-12-23T13:26:08Z |
| format | Article |
| id | doaj.art-971505f934a942c7911e9f873abff760 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 |
| language | English |
| last_indexed | 2024-12-23T13:26:08Z |
| publishDate | 2013-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-971505f934a942c7911e9f873abff7602022-12-21T17:45:19ZengBMCBiological Research0716-97602013-01-0146220120610.4067/S0716-97602013000200012S0716-97602013000200012Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)Zhengquan Gao0Chunxiao Meng1Hongzheng Gao2Xiaowen Zhang3Dong Xu4Yuanfeng Su5Yuanyuan Wang6Yuren Zhao7Naihao Ye8Shandong University of TechnologyShandong University of TechnologyShandong University of TechnologyChinese Academy of Fishery SciencesChinese Academy of Fishery SciencesShandong University of TechnologyShandong University of TechnologyShandong University of TechnologyChinese Academy of Fishery SciencesThe fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demonstrated that both 25 and 50 mg/L EBR could increase astaxanthin productivity and the eight carotenogenic genes were up-regulated by EBR with different expression profiles. Moreover, EBR25 induction had a greater influence on the transcriptional expression of ipi-1, ipi-2, crtR-B, lyc and crtO (> 5- fold up-regulation) than on psy, pds, bkt; EBR50 treatment had a greater effect on the transcriptional expression of ipi-2, pds, lyc, crtR-B, bkt and crtO than on ipi-1 and psy. Furthermore, astaxanthin biosynthesis under EBR was up-regulated mainly by ipi1־ and psy at the post-transcriptional level, pds, lyc, crtR-B, bkt and crtO at the transcriptional level and ipi-2 at both levels.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200012&lng=en&tlng=enHaematococcus pluvialisastaxanthin2, 4-Epibrassinolide (EBR)carotenoid genesreal-time fluorescence quantitative PCR (qRT-PCR) |
| spellingShingle | Zhengquan Gao Chunxiao Meng Hongzheng Gao Xiaowen Zhang Dong Xu Yuanfeng Su Yuanyuan Wang Yuren Zhao Naihao Ye Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR) Biological Research Haematococcus pluvialis astaxanthin 2, 4-Epibrassinolide (EBR) carotenoid genes real-time fluorescence quantitative PCR (qRT-PCR) |
| title | Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR) |
| title_full | Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR) |
| title_fullStr | Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR) |
| title_full_unstemmed | Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR) |
| title_short | Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR) |
| title_sort | analysis of mrna expression profiles of carotenogenesis and astaxanthin production of haematococcus pluvialis under exogenous 2 4 epibrassinolide ebr |
| topic | Haematococcus pluvialis astaxanthin 2, 4-Epibrassinolide (EBR) carotenoid genes real-time fluorescence quantitative PCR (qRT-PCR) |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200012&lng=en&tlng=en |
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