Investigation of Halohydrins Degradation by Whole Cells and Cell-free Extract of Pseudomonas putida DSM 437: A Kinetic Approach
The biodegradation of two halohydrins (1,3-dichloro-2-propanol and 3-chloro-1,2-propanediol) by P. putida DSM 437 was investigated. Intact cells of previously acclimatized P. putida DSM 437 as well as cell-free extracts were used in order to study the degradation kinetics. When...
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| Format: | Article |
| Language: | English |
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Croatian Society of Chemical Engineers
2017-10-01
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| Series: | Chemical and Biochemical Engineering Quarterly |
| Subjects: | |
| Online Access: | http://silverstripe.fkit.hr/cabeq/assets/Uploads/05-3-17.pdf |
| _version_ | 1798044998227197952 |
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| author | A. Konti D. Mamma D. Kekos |
| author_facet | A. Konti D. Mamma D. Kekos |
| author_sort | A. Konti |
| collection | DOAJ |
| description | The biodegradation of two halohydrins (1,3-dichloro-2-propanol and 3-chloro-1,2-propanediol) by P. putida DSM 437 was investigated. Intact cells of previously acclimatized P. putida DSM 437 as well as cell-free extracts were used in order to study the degradation kinetics. When whole cells were used, a maximum biodegradation rate of 3-CPD (vmax = 1.28.10–5 mmol mg–1 DCW h–1) was determined, which was more than 4 times higher than that of 1,3-DCP. However, the affinity towards both halohydrins (Km) was practically the same. When using cell-free extract, the apparent vmax and
Km values for 1,3-DCP were estimated at 9.61.10–6 mmol mg–1
protein h–1 and 8.00 mM, respectively, while for 3-CPD the corresponding values were 2.42.10–5 mmol mg–1 protein h–1
and 9.07 mM.
GC-MS analysis of cell-free extracts samples spiked with 1,3-DCP revealed the presence of 3-CPD and glycerol, intermediates of 1,3-DCP degradation pathway. 3-CPD degradation was strongly inhibited by the presence of epichlorohydrin and to a lesser extent by glycidol, intermediates of dehalogenation pathway. |
| first_indexed | 2024-04-11T23:13:26Z |
| format | Article |
| id | doaj.art-97156311c36c49e8adf9e69ae003bb7f |
| institution | Directory Open Access Journal |
| issn | 0352-9568 1846-5153 |
| language | English |
| last_indexed | 2024-04-11T23:13:26Z |
| publishDate | 2017-10-01 |
| publisher | Croatian Society of Chemical Engineers |
| record_format | Article |
| series | Chemical and Biochemical Engineering Quarterly |
| spelling | doaj.art-97156311c36c49e8adf9e69ae003bb7f2022-12-22T03:57:42ZengCroatian Society of Chemical EngineersChemical and Biochemical Engineering Quarterly0352-95681846-51532017-10-0131323324010.15255/CABEQ.2016.924Investigation of Halohydrins Degradation by Whole Cells and Cell-free Extract of Pseudomonas putida DSM 437: A Kinetic ApproachA. Konti0D. Mamma1D. Kekos2Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15773, Athens, GreeceBiotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15773, Athens, GreeceBiotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15773, Athens, GreeceThe biodegradation of two halohydrins (1,3-dichloro-2-propanol and 3-chloro-1,2-propanediol) by P. putida DSM 437 was investigated. Intact cells of previously acclimatized P. putida DSM 437 as well as cell-free extracts were used in order to study the degradation kinetics. When whole cells were used, a maximum biodegradation rate of 3-CPD (vmax = 1.28.10–5 mmol mg–1 DCW h–1) was determined, which was more than 4 times higher than that of 1,3-DCP. However, the affinity towards both halohydrins (Km) was practically the same. When using cell-free extract, the apparent vmax and Km values for 1,3-DCP were estimated at 9.61.10–6 mmol mg–1 protein h–1 and 8.00 mM, respectively, while for 3-CPD the corresponding values were 2.42.10–5 mmol mg–1 protein h–1 and 9.07 mM. GC-MS analysis of cell-free extracts samples spiked with 1,3-DCP revealed the presence of 3-CPD and glycerol, intermediates of 1,3-DCP degradation pathway. 3-CPD degradation was strongly inhibited by the presence of epichlorohydrin and to a lesser extent by glycidol, intermediates of dehalogenation pathway.http://silverstripe.fkit.hr/cabeq/assets/Uploads/05-3-17.pdfbiodegradationhalohydrinsP. putidakineticswhole cellscell-free extracts |
| spellingShingle | A. Konti D. Mamma D. Kekos Investigation of Halohydrins Degradation by Whole Cells and Cell-free Extract of Pseudomonas putida DSM 437: A Kinetic Approach Chemical and Biochemical Engineering Quarterly biodegradation halohydrins P. putida kinetics whole cells cell-free extracts |
| title | Investigation of Halohydrins Degradation by Whole Cells and Cell-free Extract of Pseudomonas putida DSM 437: A Kinetic Approach |
| title_full | Investigation of Halohydrins Degradation by Whole Cells and Cell-free Extract of Pseudomonas putida DSM 437: A Kinetic Approach |
| title_fullStr | Investigation of Halohydrins Degradation by Whole Cells and Cell-free Extract of Pseudomonas putida DSM 437: A Kinetic Approach |
| title_full_unstemmed | Investigation of Halohydrins Degradation by Whole Cells and Cell-free Extract of Pseudomonas putida DSM 437: A Kinetic Approach |
| title_short | Investigation of Halohydrins Degradation by Whole Cells and Cell-free Extract of Pseudomonas putida DSM 437: A Kinetic Approach |
| title_sort | investigation of halohydrins degradation by whole cells and cell free extract of pseudomonas putida dsm 437 a kinetic approach |
| topic | biodegradation halohydrins P. putida kinetics whole cells cell-free extracts |
| url | http://silverstripe.fkit.hr/cabeq/assets/Uploads/05-3-17.pdf |
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