The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice
Abstract Subchondral bone degeneration is the main pathological change during temporomandibular joint (TMJ) osteoarthritis (OA) development. Netrin-1, an axon-guiding factor, might play roles in OA development and pain. The purpose of this study was to investigate the expression of Netrin-1 in TMJ O...
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| Format: | Article |
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Nature Portfolio
2021-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-95251-9 |
| _version_ | 1818422304269402112 |
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| author | Mian Xiao Zhihui Hu Henghua Jiang Cheng Li Huilin Guo Wei Fang Xing Long |
| author_facet | Mian Xiao Zhihui Hu Henghua Jiang Cheng Li Huilin Guo Wei Fang Xing Long |
| author_sort | Mian Xiao |
| collection | DOAJ |
| description | Abstract Subchondral bone degeneration is the main pathological change during temporomandibular joint (TMJ) osteoarthritis (OA) development. Netrin-1, an axon-guiding factor, might play roles in OA development and pain. The purpose of this study was to investigate the expression of Netrin-1 in TMJ OA and its possible role in the progression of TMJ OA and pain. The synovial fluids of temporomandibular joint disorders (TMDs) patients were collected for Netrin-1 by enzyme linked immunosorbent assay (ELISA). TMJ OA model was built by MIA joint injection, and then the von Frey test, hematoxylin & eosin (H&E) staining, toluidine blue (TB) staining, immunohistochemical (IHC) staining and micro-CT were performed. After induction of osteoclast differentiation of raw264.7 cells, immunofluorescence (IF) was used to detect the Netrin-1 and its receptors on osteoclast membrane. The concentration of Netrin-1 increased in the synovial fluid of TMJ OA patients. After MIA injection to TMJ, the head withdrawal threshold (HWT) was significantly decreased. Microscopically, the structural disorder of subchondral bone was the most obvious at the 2nd week after MIA injection. In addition, Netrin-1 expression increased in the subchondral bone at the 2nd week after MIA injection. In vitro, the expressions of Netrin-1 and its receptor Unc5B were upregulated on the osteoclast membrane. Netrin-1 might be an important regulator during bone degeneration and pain in the process of TMJ OA. |
| first_indexed | 2024-12-14T13:24:07Z |
| format | Article |
| id | doaj.art-9717152f4baa476ab456221a1025ca92 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T13:24:07Z |
| publishDate | 2021-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9717152f4baa476ab456221a1025ca922022-12-21T22:59:52ZengNature PortfolioScientific Reports2045-23222021-08-0111111010.1038/s41598-021-95251-9The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in miceMian Xiao0Zhihui Hu1Henghua Jiang2Cheng Li3Huilin Guo4Wei Fang5Xing Long6State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan UniversityState Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan UniversityState Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan UniversityDepartment of Stomatology, Zhongnan Hospital of Wuhan UniversityState Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan UniversityState Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan UniversityState Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan UniversityAbstract Subchondral bone degeneration is the main pathological change during temporomandibular joint (TMJ) osteoarthritis (OA) development. Netrin-1, an axon-guiding factor, might play roles in OA development and pain. The purpose of this study was to investigate the expression of Netrin-1 in TMJ OA and its possible role in the progression of TMJ OA and pain. The synovial fluids of temporomandibular joint disorders (TMDs) patients were collected for Netrin-1 by enzyme linked immunosorbent assay (ELISA). TMJ OA model was built by MIA joint injection, and then the von Frey test, hematoxylin & eosin (H&E) staining, toluidine blue (TB) staining, immunohistochemical (IHC) staining and micro-CT were performed. After induction of osteoclast differentiation of raw264.7 cells, immunofluorescence (IF) was used to detect the Netrin-1 and its receptors on osteoclast membrane. The concentration of Netrin-1 increased in the synovial fluid of TMJ OA patients. After MIA injection to TMJ, the head withdrawal threshold (HWT) was significantly decreased. Microscopically, the structural disorder of subchondral bone was the most obvious at the 2nd week after MIA injection. In addition, Netrin-1 expression increased in the subchondral bone at the 2nd week after MIA injection. In vitro, the expressions of Netrin-1 and its receptor Unc5B were upregulated on the osteoclast membrane. Netrin-1 might be an important regulator during bone degeneration and pain in the process of TMJ OA.https://doi.org/10.1038/s41598-021-95251-9 |
| spellingShingle | Mian Xiao Zhihui Hu Henghua Jiang Cheng Li Huilin Guo Wei Fang Xing Long The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice Scientific Reports |
| title | The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice |
| title_full | The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice |
| title_fullStr | The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice |
| title_full_unstemmed | The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice |
| title_short | The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice |
| title_sort | expression of netrin 1 in the mia induced osteoarthritic temporomandibular joint in mice |
| url | https://doi.org/10.1038/s41598-021-95251-9 |
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