A Portable Device for LAMP Based Detection of SARS-CoV-2
This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the sampl...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-09-01
|
Series: | Micromachines |
Subjects: | |
Online Access: | https://www.mdpi.com/2072-666X/12/10/1151 |
_version_ | 1797513837360971776 |
---|---|
author | Kamalalayam Rajan Sreejith Muhammad Umer Larissa Dirr Benjamin Bailly Patrice Guillon Mark von Itzstein Narshone Soda Surasak Kasetsirikul Muhammad J. A. Shiddiky Nam-Trung Nguyen |
author_facet | Kamalalayam Rajan Sreejith Muhammad Umer Larissa Dirr Benjamin Bailly Patrice Guillon Mark von Itzstein Narshone Soda Surasak Kasetsirikul Muhammad J. A. Shiddiky Nam-Trung Nguyen |
author_sort | Kamalalayam Rajan Sreejith |
collection | DOAJ |
description | This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 min using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard quantitative Polymerase Chain Reaction(qPCR) machine (~10–20 min vs. >38 min) for 1 × 10<sup>2</sup> starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) detection and patient management. |
first_indexed | 2024-03-10T06:23:13Z |
format | Article |
id | doaj.art-971f60764ea64f54ad56d4d30a8017b2 |
institution | Directory Open Access Journal |
issn | 2072-666X |
language | English |
last_indexed | 2024-03-10T06:23:13Z |
publishDate | 2021-09-01 |
publisher | MDPI AG |
record_format | Article |
series | Micromachines |
spelling | doaj.art-971f60764ea64f54ad56d4d30a8017b22023-11-22T19:10:35ZengMDPI AGMicromachines2072-666X2021-09-011210115110.3390/mi12101151A Portable Device for LAMP Based Detection of SARS-CoV-2Kamalalayam Rajan Sreejith0Muhammad Umer1Larissa Dirr2Benjamin Bailly3Patrice Guillon4Mark von Itzstein5Narshone Soda6Surasak Kasetsirikul7Muhammad J. A. Shiddiky8Nam-Trung Nguyen9Queensland Micro- and Nanotechnology Centre, Griffith University, 170 Kessels Road, Nathan, QLD 4111, AustraliaQueensland Micro- and Nanotechnology Centre, Griffith University, 170 Kessels Road, Nathan, QLD 4111, AustraliaInstitute for Glycomics, Gold Coast Campus, Griffith University, 1 Parklands Drive, Southport, QLD 4222, AustraliaInstitute for Glycomics, Gold Coast Campus, Griffith University, 1 Parklands Drive, Southport, QLD 4222, AustraliaInstitute for Glycomics, Gold Coast Campus, Griffith University, 1 Parklands Drive, Southport, QLD 4222, AustraliaInstitute for Glycomics, Gold Coast Campus, Griffith University, 1 Parklands Drive, Southport, QLD 4222, AustraliaQueensland Micro- and Nanotechnology Centre, Griffith University, 170 Kessels Road, Nathan, QLD 4111, AustraliaQueensland Micro- and Nanotechnology Centre, Griffith University, 170 Kessels Road, Nathan, QLD 4111, AustraliaQueensland Micro- and Nanotechnology Centre, Griffith University, 170 Kessels Road, Nathan, QLD 4111, AustraliaQueensland Micro- and Nanotechnology Centre, Griffith University, 170 Kessels Road, Nathan, QLD 4111, AustraliaThis paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 min using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard quantitative Polymerase Chain Reaction(qPCR) machine (~10–20 min vs. >38 min) for 1 × 10<sup>2</sup> starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) detection and patient management.https://www.mdpi.com/2072-666X/12/10/1151severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2)loop-mediated isothermal amplificationportable device |
spellingShingle | Kamalalayam Rajan Sreejith Muhammad Umer Larissa Dirr Benjamin Bailly Patrice Guillon Mark von Itzstein Narshone Soda Surasak Kasetsirikul Muhammad J. A. Shiddiky Nam-Trung Nguyen A Portable Device for LAMP Based Detection of SARS-CoV-2 Micromachines severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) loop-mediated isothermal amplification portable device |
title | A Portable Device for LAMP Based Detection of SARS-CoV-2 |
title_full | A Portable Device for LAMP Based Detection of SARS-CoV-2 |
title_fullStr | A Portable Device for LAMP Based Detection of SARS-CoV-2 |
title_full_unstemmed | A Portable Device for LAMP Based Detection of SARS-CoV-2 |
title_short | A Portable Device for LAMP Based Detection of SARS-CoV-2 |
title_sort | portable device for lamp based detection of sars cov 2 |
topic | severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) loop-mediated isothermal amplification portable device |
url | https://www.mdpi.com/2072-666X/12/10/1151 |
work_keys_str_mv | AT kamalalayamrajansreejith aportabledeviceforlampbaseddetectionofsarscov2 AT muhammadumer aportabledeviceforlampbaseddetectionofsarscov2 AT larissadirr aportabledeviceforlampbaseddetectionofsarscov2 AT benjaminbailly aportabledeviceforlampbaseddetectionofsarscov2 AT patriceguillon aportabledeviceforlampbaseddetectionofsarscov2 AT markvonitzstein aportabledeviceforlampbaseddetectionofsarscov2 AT narshonesoda aportabledeviceforlampbaseddetectionofsarscov2 AT surasakkasetsirikul aportabledeviceforlampbaseddetectionofsarscov2 AT muhammadjashiddiky aportabledeviceforlampbaseddetectionofsarscov2 AT namtrungnguyen aportabledeviceforlampbaseddetectionofsarscov2 AT kamalalayamrajansreejith portabledeviceforlampbaseddetectionofsarscov2 AT muhammadumer portabledeviceforlampbaseddetectionofsarscov2 AT larissadirr portabledeviceforlampbaseddetectionofsarscov2 AT benjaminbailly portabledeviceforlampbaseddetectionofsarscov2 AT patriceguillon portabledeviceforlampbaseddetectionofsarscov2 AT markvonitzstein portabledeviceforlampbaseddetectionofsarscov2 AT narshonesoda portabledeviceforlampbaseddetectionofsarscov2 AT surasakkasetsirikul portabledeviceforlampbaseddetectionofsarscov2 AT muhammadjashiddiky portabledeviceforlampbaseddetectionofsarscov2 AT namtrungnguyen portabledeviceforlampbaseddetectionofsarscov2 |