Molecular detection of mouse hepatitis virus in laboratory mouse colonies
The animal health monitoring is required to issue health certificates. The viral hepatitis virus is one of the most important infectious agents in mice breeding colonies. This research used RT-PCR to identify contaminations to mouse hepatitis virus. 18 out of 29 specimens were found to be infected,...
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| Format: | Article |
| Language: | English |
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Ferdowsi University of Mashhad
2019-02-01
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| Series: | The Iranian Journal of Veterinary Science and Technology |
| Subjects: | |
| Online Access: | https://ijvst.um.ac.ir/article_30476_d93704a7a0dc3489e176c2dba910a269.pdf |
| _version_ | 1798023800614289408 |
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| author | Roozbeh Fallahi Fatemeh Abedini Gholam Reza Shokri |
| author_facet | Roozbeh Fallahi Fatemeh Abedini Gholam Reza Shokri |
| author_sort | Roozbeh Fallahi |
| collection | DOAJ |
| description | The animal health monitoring is required to issue health certificates. The viral hepatitis virus is one of the most important infectious agents in mice breeding colonies. This research used RT-PCR to identify contaminations to mouse hepatitis virus. 18 out of 29 specimens were found to be infected, a prevalence of 62%. PCR product was purified and sequenced. Phylogenetic analysis revealed that the identified strain in this study was closely related to a strain reported from France. In the conventional system, contamination with different infectious agents is inevitable, thus it is better to replace the contaminated colonies with clean animals. |
| first_indexed | 2024-04-11T17:52:18Z |
| format | Article |
| id | doaj.art-97225fe61ac44f449676c5e4a6cc0069 |
| institution | Directory Open Access Journal |
| issn | 2008-465X 2423-6306 |
| language | English |
| last_indexed | 2024-04-11T17:52:18Z |
| publishDate | 2019-02-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | The Iranian Journal of Veterinary Science and Technology |
| spelling | doaj.art-97225fe61ac44f449676c5e4a6cc00692022-12-22T04:11:02ZengFerdowsi University of MashhadThe Iranian Journal of Veterinary Science and Technology2008-465X2423-63062019-02-01112697210.22067/veterinary.v11i2.7763130476Molecular detection of mouse hepatitis virus in laboratory mouse coloniesRoozbeh Fallahi0Fatemeh Abedini1Gholam Reza Shokri2-Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.Research and Development Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, IranThe animal health monitoring is required to issue health certificates. The viral hepatitis virus is one of the most important infectious agents in mice breeding colonies. This research used RT-PCR to identify contaminations to mouse hepatitis virus. 18 out of 29 specimens were found to be infected, a prevalence of 62%. PCR product was purified and sequenced. Phylogenetic analysis revealed that the identified strain in this study was closely related to a strain reported from France. In the conventional system, contamination with different infectious agents is inevitable, thus it is better to replace the contaminated colonies with clean animals.https://ijvst.um.ac.ir/article_30476_d93704a7a0dc3489e176c2dba910a269.pdfmolecular detectionmousehepatitis virus |
| spellingShingle | Roozbeh Fallahi Fatemeh Abedini Gholam Reza Shokri Molecular detection of mouse hepatitis virus in laboratory mouse colonies The Iranian Journal of Veterinary Science and Technology molecular detection mouse hepatitis virus |
| title | Molecular detection of mouse hepatitis virus in laboratory mouse colonies |
| title_full | Molecular detection of mouse hepatitis virus in laboratory mouse colonies |
| title_fullStr | Molecular detection of mouse hepatitis virus in laboratory mouse colonies |
| title_full_unstemmed | Molecular detection of mouse hepatitis virus in laboratory mouse colonies |
| title_short | Molecular detection of mouse hepatitis virus in laboratory mouse colonies |
| title_sort | molecular detection of mouse hepatitis virus in laboratory mouse colonies |
| topic | molecular detection mouse hepatitis virus |
| url | https://ijvst.um.ac.ir/article_30476_d93704a7a0dc3489e176c2dba910a269.pdf |
| work_keys_str_mv | AT roozbehfallahi moleculardetectionofmousehepatitisvirusinlaboratorymousecolonies AT fatemehabedini moleculardetectionofmousehepatitisvirusinlaboratorymousecolonies AT gholamrezashokri moleculardetectionofmousehepatitisvirusinlaboratorymousecolonies |