Efficient Approach for the Extraction and Identification of Red Pigment from Zanthoxylum bungeanum Maxim and Its Antioxidant Activity
Red pigment (RP) was extracted from the peels of Zanthoxylum bungeanum Maxim (PZB) by ultrasonic-assisted extraction (UAE) in this work. Box–Behnken design–response surface methodology (BBD-RSM) was employed to research the efficiency of the RP extraction. Based on the optimizati...
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MDPI AG
2018-05-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/5/1109 |
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| author | Xi Chen Zhiqiang Wei Lei Zhu Xing Yuan Daneng Wei Wei Peng Chunjie Wu |
| author_facet | Xi Chen Zhiqiang Wei Lei Zhu Xing Yuan Daneng Wei Wei Peng Chunjie Wu |
| author_sort | Xi Chen |
| collection | DOAJ |
| description | Red pigment (RP) was extracted from the peels of Zanthoxylum bungeanum Maxim (PZB) by ultrasonic-assisted extraction (UAE) in this work. Box–Behnken design–response surface methodology (BBD-RSM) was employed to research the efficiency of the RP extraction. Based on the optimization of RSM, results showed that the optimal extraction conditions were as follows: liquid–solid ratio of 31 mL/g, extraction time of 41 min, and extraction temperature of 27 °C, and under these conditions, the actual absorbance value was 0.615 ± 0.13%, highly agreeing with the predicted value by the model. Furthermore, ultra-performance liquid chromatography–mass spectrometry (UPLC-MS) was used to separate and analyze RP. The compounds of RP were mainly flavonoids, and there were five compounds detected for the first time in PZB. In addition, RP showed significant antioxidant activities in vitro, which could be developed for anti-aging candidate drugs and functional foods. In conclusion, ultrasound-assisted extraction with BBD-RSM and chromatographic separation technology with UPLC-MS are efficient strategies for the isolation and identification of RP from PZB. |
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| language | English |
| last_indexed | 2024-04-13T12:21:42Z |
| publishDate | 2018-05-01 |
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| spelling | doaj.art-9726658cc2ec4c4c87e134507af494732022-12-22T02:47:12ZengMDPI AGMolecules1420-30492018-05-01235110910.3390/molecules23051109molecules23051109Efficient Approach for the Extraction and Identification of Red Pigment from Zanthoxylum bungeanum Maxim and Its Antioxidant ActivityXi Chen0Zhiqiang Wei1Lei Zhu2Xing Yuan3Daneng Wei4Wei Peng5Chunjie Wu6College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaRed pigment (RP) was extracted from the peels of Zanthoxylum bungeanum Maxim (PZB) by ultrasonic-assisted extraction (UAE) in this work. Box–Behnken design–response surface methodology (BBD-RSM) was employed to research the efficiency of the RP extraction. Based on the optimization of RSM, results showed that the optimal extraction conditions were as follows: liquid–solid ratio of 31 mL/g, extraction time of 41 min, and extraction temperature of 27 °C, and under these conditions, the actual absorbance value was 0.615 ± 0.13%, highly agreeing with the predicted value by the model. Furthermore, ultra-performance liquid chromatography–mass spectrometry (UPLC-MS) was used to separate and analyze RP. The compounds of RP were mainly flavonoids, and there were five compounds detected for the first time in PZB. In addition, RP showed significant antioxidant activities in vitro, which could be developed for anti-aging candidate drugs and functional foods. In conclusion, ultrasound-assisted extraction with BBD-RSM and chromatographic separation technology with UPLC-MS are efficient strategies for the isolation and identification of RP from PZB.http://www.mdpi.com/1420-3049/23/5/1109Zanthoxylum bungeanum Maximred pigmentultrasound-assisted extractionBBD-RSMUPLC-MSantioxidant activity |
| spellingShingle | Xi Chen Zhiqiang Wei Lei Zhu Xing Yuan Daneng Wei Wei Peng Chunjie Wu Efficient Approach for the Extraction and Identification of Red Pigment from Zanthoxylum bungeanum Maxim and Its Antioxidant Activity Molecules Zanthoxylum bungeanum Maxim red pigment ultrasound-assisted extraction BBD-RSM UPLC-MS antioxidant activity |
| title | Efficient Approach for the Extraction and Identification of Red Pigment from Zanthoxylum bungeanum Maxim and Its Antioxidant Activity |
| title_full | Efficient Approach for the Extraction and Identification of Red Pigment from Zanthoxylum bungeanum Maxim and Its Antioxidant Activity |
| title_fullStr | Efficient Approach for the Extraction and Identification of Red Pigment from Zanthoxylum bungeanum Maxim and Its Antioxidant Activity |
| title_full_unstemmed | Efficient Approach for the Extraction and Identification of Red Pigment from Zanthoxylum bungeanum Maxim and Its Antioxidant Activity |
| title_short | Efficient Approach for the Extraction and Identification of Red Pigment from Zanthoxylum bungeanum Maxim and Its Antioxidant Activity |
| title_sort | efficient approach for the extraction and identification of red pigment from zanthoxylum bungeanum maxim and its antioxidant activity |
| topic | Zanthoxylum bungeanum Maxim red pigment ultrasound-assisted extraction BBD-RSM UPLC-MS antioxidant activity |
| url | http://www.mdpi.com/1420-3049/23/5/1109 |
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