Development of Biomaterials Based on Biomimetic Trace Elements Co-Doped Hydroxyapatite: Physical, In Vitro Osteoblast-like Cell Growth and In Vivo Cytotoxicity in Zebrafish Studies
Synthesized hydroxyapatite (sHA)—calcium phosphate (CaP) based biomaterials play a vital role and have been widely used in the process of bone regeneration for bone defect repair, due to their similarities to the inorganic components of human bones. However, for bone tissue engineering purpose, the...
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MDPI AG
2023-01-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/2/255 |
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| author | Tanatsaparn Tithito Siwapech Sillapaprayoon Wittaya Pimtong Jirawan Thongbunchoo Narattaphol Charoenphandhu Nateetip Krishnamra Aurachat Lert-itthiporn Weerakanya Maneeprakorn Weeraphat Pon-On |
| author_facet | Tanatsaparn Tithito Siwapech Sillapaprayoon Wittaya Pimtong Jirawan Thongbunchoo Narattaphol Charoenphandhu Nateetip Krishnamra Aurachat Lert-itthiporn Weerakanya Maneeprakorn Weeraphat Pon-On |
| author_sort | Tanatsaparn Tithito |
| collection | DOAJ |
| description | Synthesized hydroxyapatite (sHA)—calcium phosphate (CaP) based biomaterials play a vital role and have been widely used in the process of bone regeneration for bone defect repair, due to their similarities to the inorganic components of human bones. However, for bone tissue engineering purpose, the composite components, physical and biological properties, efficacy and safety of sHA still need further improvements. In this work, we synthesized inhomogeneous hydroxyapatite based on biomimetic trace elements (Mg, Fe, Zn, Mn, Cu, Ni, Mo, Sr, Co, BO<sub>3</sub><sup>3−</sup>, and CO<sub>3</sub><sup>2−</sup>) co-doped into HA (THA) (Ca<sub>10−<i>δ</i></sub>M<i><sub>δ</sub></i>(PO<sub>4</sub>)<sub>5.5</sub>(CO<sub>3</sub>)<sub>0.5</sub>(OH)<sub>2</sub>, M = trace elements) via co-precipitation from an ionic solution. The physical properties, their bioactivities using in vitro osteoblast cells, and in vivo cytotoxicity using zebrafish were studied. By introducing biomimetic trace elements, the as-prepared THA samples showed nanorod (needle-like) structures, having a positively charged surface (6.49 meV), and showing paramagnetic behavior. The bioactivity studies demonstrated that the THA substrate can induce apatite particles to cover its surface and be in contact with surrounding simulated body fluid (SBF). In vitro biological assays revealed that the osteoblast-like UMR-106 cells were well-attached with growth and proliferation on the substrate’s surface. Upon differentiation, enhanced ALP (alkaline phosphatase) activity was observed for bone cells on the surface of the THA compared with that on the control substrates (sHA). The in vivo performance in embryonic zebrafish studies showed that the synthesized THA particles are nontoxic based on the measurements of essential parameters such as survivability, hatching rate, and the morphology of the embryo. The mechanism of the ions release profile using digital conductivity measurement revealed that sustained controlled release was successfully achieved. These preliminary results indicated that the synthesized THA could be a promising material for potential practical applications in bone tissue engineering. |
| first_indexed | 2024-03-09T11:33:43Z |
| format | Article |
| id | doaj.art-9729406bf6604d718355a5a196093cf9 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T11:33:43Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-9729406bf6604d718355a5a196093cf92023-11-30T23:47:18ZengMDPI AGNanomaterials2079-49912023-01-0113225510.3390/nano13020255Development of Biomaterials Based on Biomimetic Trace Elements Co-Doped Hydroxyapatite: Physical, In Vitro Osteoblast-like Cell Growth and In Vivo Cytotoxicity in Zebrafish StudiesTanatsaparn Tithito0Siwapech Sillapaprayoon1Wittaya Pimtong2Jirawan Thongbunchoo3Narattaphol Charoenphandhu4Nateetip Krishnamra5Aurachat Lert-itthiporn6Weerakanya Maneeprakorn7Weeraphat Pon-On8Department of Physics, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandNano Environmental and Health Safety Research Team, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang 12120, ThailandNano Environmental and Health Safety Research Team, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang 12120, ThailandCenter of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok 10400, ThailandCenter of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok 10400, ThailandCenter of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok 10400, ThailandResponsive Nanomaterials Research Team, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang 12120, ThailandResponsive Nanomaterials Research Team, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang 12120, ThailandDepartment of Physics, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandSynthesized hydroxyapatite (sHA)—calcium phosphate (CaP) based biomaterials play a vital role and have been widely used in the process of bone regeneration for bone defect repair, due to their similarities to the inorganic components of human bones. However, for bone tissue engineering purpose, the composite components, physical and biological properties, efficacy and safety of sHA still need further improvements. In this work, we synthesized inhomogeneous hydroxyapatite based on biomimetic trace elements (Mg, Fe, Zn, Mn, Cu, Ni, Mo, Sr, Co, BO<sub>3</sub><sup>3−</sup>, and CO<sub>3</sub><sup>2−</sup>) co-doped into HA (THA) (Ca<sub>10−<i>δ</i></sub>M<i><sub>δ</sub></i>(PO<sub>4</sub>)<sub>5.5</sub>(CO<sub>3</sub>)<sub>0.5</sub>(OH)<sub>2</sub>, M = trace elements) via co-precipitation from an ionic solution. The physical properties, their bioactivities using in vitro osteoblast cells, and in vivo cytotoxicity using zebrafish were studied. By introducing biomimetic trace elements, the as-prepared THA samples showed nanorod (needle-like) structures, having a positively charged surface (6.49 meV), and showing paramagnetic behavior. The bioactivity studies demonstrated that the THA substrate can induce apatite particles to cover its surface and be in contact with surrounding simulated body fluid (SBF). In vitro biological assays revealed that the osteoblast-like UMR-106 cells were well-attached with growth and proliferation on the substrate’s surface. Upon differentiation, enhanced ALP (alkaline phosphatase) activity was observed for bone cells on the surface of the THA compared with that on the control substrates (sHA). The in vivo performance in embryonic zebrafish studies showed that the synthesized THA particles are nontoxic based on the measurements of essential parameters such as survivability, hatching rate, and the morphology of the embryo. The mechanism of the ions release profile using digital conductivity measurement revealed that sustained controlled release was successfully achieved. These preliminary results indicated that the synthesized THA could be a promising material for potential practical applications in bone tissue engineering.https://www.mdpi.com/2079-4991/13/2/255hydroxyapatitebiomimetic trace elementsions-doped HAbone tissue engineeringzebrafish |
| spellingShingle | Tanatsaparn Tithito Siwapech Sillapaprayoon Wittaya Pimtong Jirawan Thongbunchoo Narattaphol Charoenphandhu Nateetip Krishnamra Aurachat Lert-itthiporn Weerakanya Maneeprakorn Weeraphat Pon-On Development of Biomaterials Based on Biomimetic Trace Elements Co-Doped Hydroxyapatite: Physical, In Vitro Osteoblast-like Cell Growth and In Vivo Cytotoxicity in Zebrafish Studies Nanomaterials hydroxyapatite biomimetic trace elements ions-doped HA bone tissue engineering zebrafish |
| title | Development of Biomaterials Based on Biomimetic Trace Elements Co-Doped Hydroxyapatite: Physical, In Vitro Osteoblast-like Cell Growth and In Vivo Cytotoxicity in Zebrafish Studies |
| title_full | Development of Biomaterials Based on Biomimetic Trace Elements Co-Doped Hydroxyapatite: Physical, In Vitro Osteoblast-like Cell Growth and In Vivo Cytotoxicity in Zebrafish Studies |
| title_fullStr | Development of Biomaterials Based on Biomimetic Trace Elements Co-Doped Hydroxyapatite: Physical, In Vitro Osteoblast-like Cell Growth and In Vivo Cytotoxicity in Zebrafish Studies |
| title_full_unstemmed | Development of Biomaterials Based on Biomimetic Trace Elements Co-Doped Hydroxyapatite: Physical, In Vitro Osteoblast-like Cell Growth and In Vivo Cytotoxicity in Zebrafish Studies |
| title_short | Development of Biomaterials Based on Biomimetic Trace Elements Co-Doped Hydroxyapatite: Physical, In Vitro Osteoblast-like Cell Growth and In Vivo Cytotoxicity in Zebrafish Studies |
| title_sort | development of biomaterials based on biomimetic trace elements co doped hydroxyapatite physical in vitro osteoblast like cell growth and in vivo cytotoxicity in zebrafish studies |
| topic | hydroxyapatite biomimetic trace elements ions-doped HA bone tissue engineering zebrafish |
| url | https://www.mdpi.com/2079-4991/13/2/255 |
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