Application of deep eutectic solvents in protein extraction and purification
Deep eutectic solvents (DESs) are a mixture of hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA) molecules that can consist, respectively, of natural plant metabolites such as sugars, carboxylic acids, amino acids, and ionic molecules, which are for the vast majority ammonium salts. Media s...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2022.912411/full |
| _version_ | 1818066002606292992 |
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| author | Hou Bowen Rabia Durrani André Delavault Erwann Durand Erwann Durand Jiang Chenyu Long Yiyang Song Lili Song Jian Huan Weiwei Gao Fei |
| author_facet | Hou Bowen Rabia Durrani André Delavault Erwann Durand Erwann Durand Jiang Chenyu Long Yiyang Song Lili Song Jian Huan Weiwei Gao Fei |
| author_sort | Hou Bowen |
| collection | DOAJ |
| description | Deep eutectic solvents (DESs) are a mixture of hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA) molecules that can consist, respectively, of natural plant metabolites such as sugars, carboxylic acids, amino acids, and ionic molecules, which are for the vast majority ammonium salts. Media such as DESs are modular tools of sustainability that can be pointed toward the extraction of bioactive molecules due to their excellent physicochemical properties, their relatively low price, and accessibility. The present review focuses on the application of DESs for protein extraction and purification. The in-depth effects and principles that apply to DES-mediated extraction using various renewable biomasses will be discussed as well. One of the most important observations being made is that DESs have a clear ability to maintain the biological and/or functional activity of the extracted proteins, as well as increase their stability compared to traditional solvents. They demonstrate true potential for a reproducible but more importantly, scalable protein extraction and purification compared to traditional methods while enabling waste valorization in some particular cases. |
| first_indexed | 2024-12-10T15:00:52Z |
| format | Article |
| id | doaj.art-972c413b96994e779f819aa78172ae22 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-10T15:00:52Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-972c413b96994e779f819aa78172ae222022-12-22T01:44:10ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462022-09-011010.3389/fchem.2022.912411912411Application of deep eutectic solvents in protein extraction and purificationHou Bowen0Rabia Durrani1André Delavault2Erwann Durand3Erwann Durand4Jiang Chenyu5Long Yiyang6Song Lili7Song Jian8Huan Weiwei9Gao Fei10Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an, Zhejiang, ChinaTechnical Biology, Institute of Process Engineering in Life Sciences II, Karlsruhe Institute of Technology, Karlsruhe, GermanyCIRAD, UMR QUALISUD, Montpellier, FranceQualisud, Univ Montpellier, Avignon Université, CIRAD, Institut Agro, Université de la Réunion, Montpellier, FranceState Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an, Zhejiang, ChinaZhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an, Zhejiang, ChinaZhejiang Provincial Key Laboratory of Chemical Utilization of Forestry Biomass, College of Chemistry and Materials Engineering, Zhejiang A & F University, Hangzhou, Zhejiang, ChinaZhejiang Provincial Key Laboratory of Chemical Utilization of Forestry Biomass, College of Chemistry and Materials Engineering, Zhejiang A & F University, Hangzhou, Zhejiang, ChinaZhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou, ChinaDeep eutectic solvents (DESs) are a mixture of hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA) molecules that can consist, respectively, of natural plant metabolites such as sugars, carboxylic acids, amino acids, and ionic molecules, which are for the vast majority ammonium salts. Media such as DESs are modular tools of sustainability that can be pointed toward the extraction of bioactive molecules due to their excellent physicochemical properties, their relatively low price, and accessibility. The present review focuses on the application of DESs for protein extraction and purification. The in-depth effects and principles that apply to DES-mediated extraction using various renewable biomasses will be discussed as well. One of the most important observations being made is that DESs have a clear ability to maintain the biological and/or functional activity of the extracted proteins, as well as increase their stability compared to traditional solvents. They demonstrate true potential for a reproducible but more importantly, scalable protein extraction and purification compared to traditional methods while enabling waste valorization in some particular cases.https://www.frontiersin.org/articles/10.3389/fchem.2022.912411/fulldeep eutectic solventproteinextractiongreen solventbiodegradability |
| spellingShingle | Hou Bowen Rabia Durrani André Delavault Erwann Durand Erwann Durand Jiang Chenyu Long Yiyang Song Lili Song Jian Huan Weiwei Gao Fei Application of deep eutectic solvents in protein extraction and purification Frontiers in Chemistry deep eutectic solvent protein extraction green solvent biodegradability |
| title | Application of deep eutectic solvents in protein extraction and purification |
| title_full | Application of deep eutectic solvents in protein extraction and purification |
| title_fullStr | Application of deep eutectic solvents in protein extraction and purification |
| title_full_unstemmed | Application of deep eutectic solvents in protein extraction and purification |
| title_short | Application of deep eutectic solvents in protein extraction and purification |
| title_sort | application of deep eutectic solvents in protein extraction and purification |
| topic | deep eutectic solvent protein extraction green solvent biodegradability |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2022.912411/full |
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