Ameliorative Effect of <i>Ocimum forskolei</i> Benth on Diabetic, Apoptotic, and Adipogenic Biomarkers of Diabetic Rats and 3T3-L1 Fibroblasts Assisted by In Silico Approach
Diabetes mellitus (DM) is a complicated condition that is accompanied by a plethora of metabolic symptoms, including disturbed serum glucose and lipid profiles. Several herbs are reputed as traditional medicine to improve DM. The current study was designed to explore the chemical composition and pos...
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2022-04-01
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| author | Hany Ezzat Khalil Miada F. Abdelwahab Promise Madu Emeka Lorina I. Badger-Emeka Krishnaraj Thirugnanasambantham Hairul-Islam Mohamed Ibrahim Sara Mohamed Naguib Katsuyoshi Matsunami Nada M. Abdel-Wahab |
| author_facet | Hany Ezzat Khalil Miada F. Abdelwahab Promise Madu Emeka Lorina I. Badger-Emeka Krishnaraj Thirugnanasambantham Hairul-Islam Mohamed Ibrahim Sara Mohamed Naguib Katsuyoshi Matsunami Nada M. Abdel-Wahab |
| author_sort | Hany Ezzat Khalil |
| collection | DOAJ |
| description | Diabetes mellitus (DM) is a complicated condition that is accompanied by a plethora of metabolic symptoms, including disturbed serum glucose and lipid profiles. Several herbs are reputed as traditional medicine to improve DM. The current study was designed to explore the chemical composition and possible ameliorative effects of <i>Ocimum forskolei</i> on blood glucose and lipid profile in high-fat diet/streptozotocin-induced diabetic rats and in 3T3-L1 cell lines as a first report of its bioactivity. Histopathological study of pancreatic and adipose tissues was performed in control and treatment groups, along with quantification of glucose and lipid profiles and the assessment of NF-κB, cleaved caspase-3, BAX, and BCL2 markers in rat pancreatic tissue. Glucose uptake, adipogenic markers, DGAT1, CEBP/α, and PPARγ levels were evaluated in the 3T3-L1 cell line. Hesperidin was isolated from total methanol extract (TME). TME and hesperidin significantly controlled the glucose and lipid profile in DM rats. Glibenclamide was used as a positive control. Histopathological assessment showed that TME and hesperidin averted necrosis and infiltration in pancreatic tissues, and led to a substantial improvement in the cellular structure of adipose tissue. TME and hesperidin distinctly diminished the mRNA and protein expression of NF-κB, cleaved caspase-3, and BAX, and increased BCL2 expression (reflecting its protective and antiapoptotic actions). Interestingly, TME and hesperidin reduced glucose uptake and oxidative lipid accumulation in the 3T3-L1 cell line. TME and hesperidin reduced DGAT1, CEBP/α, and PPARγ mRNA and protein expression in 3T3-L1 cells. Moreover, docking studies supported the results via deep interaction of hesperidin with the tested biomarkers. Taken together, the current study demonstrates <i>Ocimum forskolei</i> and hesperidin as possible candidates for treating diabetes mellitus. |
| first_indexed | 2024-03-10T03:54:48Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:54:48Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-972e534168a64703a90b7e501621340f2023-11-23T08:49:50ZengMDPI AGMolecules1420-30492022-04-01279280010.3390/molecules27092800Ameliorative Effect of <i>Ocimum forskolei</i> Benth on Diabetic, Apoptotic, and Adipogenic Biomarkers of Diabetic Rats and 3T3-L1 Fibroblasts Assisted by In Silico ApproachHany Ezzat Khalil0Miada F. Abdelwahab1Promise Madu Emeka2Lorina I. Badger-Emeka3Krishnaraj Thirugnanasambantham4Hairul-Islam Mohamed Ibrahim5Sara Mohamed Naguib6Katsuyoshi Matsunami7Nada M. Abdel-Wahab8Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa 31982, Saudi ArabiaDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, EgyptDepartment of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa 31982, Saudi ArabiaDepartment of Biomedical Sciences, College of Medicine, King Faisal University, Al-Ahsa 31982, Saudi ArabiaPondicherry Centre for Biological Science and Educational Trust, Kottakuppam 605104, IndiaPondicherry Centre for Biological Science and Educational Trust, Kottakuppam 605104, IndiaDepartment of Histology and Cell Biology, Faculty of Medicine, Minia University, Minia 61111, EgyptDepartment of Pharmacognosy, Graduate School of Biomedical & Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, JapanDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, EgyptDiabetes mellitus (DM) is a complicated condition that is accompanied by a plethora of metabolic symptoms, including disturbed serum glucose and lipid profiles. Several herbs are reputed as traditional medicine to improve DM. The current study was designed to explore the chemical composition and possible ameliorative effects of <i>Ocimum forskolei</i> on blood glucose and lipid profile in high-fat diet/streptozotocin-induced diabetic rats and in 3T3-L1 cell lines as a first report of its bioactivity. Histopathological study of pancreatic and adipose tissues was performed in control and treatment groups, along with quantification of glucose and lipid profiles and the assessment of NF-κB, cleaved caspase-3, BAX, and BCL2 markers in rat pancreatic tissue. Glucose uptake, adipogenic markers, DGAT1, CEBP/α, and PPARγ levels were evaluated in the 3T3-L1 cell line. Hesperidin was isolated from total methanol extract (TME). TME and hesperidin significantly controlled the glucose and lipid profile in DM rats. Glibenclamide was used as a positive control. Histopathological assessment showed that TME and hesperidin averted necrosis and infiltration in pancreatic tissues, and led to a substantial improvement in the cellular structure of adipose tissue. TME and hesperidin distinctly diminished the mRNA and protein expression of NF-κB, cleaved caspase-3, and BAX, and increased BCL2 expression (reflecting its protective and antiapoptotic actions). Interestingly, TME and hesperidin reduced glucose uptake and oxidative lipid accumulation in the 3T3-L1 cell line. TME and hesperidin reduced DGAT1, CEBP/α, and PPARγ mRNA and protein expression in 3T3-L1 cells. Moreover, docking studies supported the results via deep interaction of hesperidin with the tested biomarkers. Taken together, the current study demonstrates <i>Ocimum forskolei</i> and hesperidin as possible candidates for treating diabetes mellitus.https://www.mdpi.com/1420-3049/27/9/2800<i>Ocimum forskolei</i>hesperidinstreptozotocindiabetes3T3-L1NF-κB |
| spellingShingle | Hany Ezzat Khalil Miada F. Abdelwahab Promise Madu Emeka Lorina I. Badger-Emeka Krishnaraj Thirugnanasambantham Hairul-Islam Mohamed Ibrahim Sara Mohamed Naguib Katsuyoshi Matsunami Nada M. Abdel-Wahab Ameliorative Effect of <i>Ocimum forskolei</i> Benth on Diabetic, Apoptotic, and Adipogenic Biomarkers of Diabetic Rats and 3T3-L1 Fibroblasts Assisted by In Silico Approach Molecules <i>Ocimum forskolei</i> hesperidin streptozotocin diabetes 3T3-L1 NF-κB |
| title | Ameliorative Effect of <i>Ocimum forskolei</i> Benth on Diabetic, Apoptotic, and Adipogenic Biomarkers of Diabetic Rats and 3T3-L1 Fibroblasts Assisted by In Silico Approach |
| title_full | Ameliorative Effect of <i>Ocimum forskolei</i> Benth on Diabetic, Apoptotic, and Adipogenic Biomarkers of Diabetic Rats and 3T3-L1 Fibroblasts Assisted by In Silico Approach |
| title_fullStr | Ameliorative Effect of <i>Ocimum forskolei</i> Benth on Diabetic, Apoptotic, and Adipogenic Biomarkers of Diabetic Rats and 3T3-L1 Fibroblasts Assisted by In Silico Approach |
| title_full_unstemmed | Ameliorative Effect of <i>Ocimum forskolei</i> Benth on Diabetic, Apoptotic, and Adipogenic Biomarkers of Diabetic Rats and 3T3-L1 Fibroblasts Assisted by In Silico Approach |
| title_short | Ameliorative Effect of <i>Ocimum forskolei</i> Benth on Diabetic, Apoptotic, and Adipogenic Biomarkers of Diabetic Rats and 3T3-L1 Fibroblasts Assisted by In Silico Approach |
| title_sort | ameliorative effect of i ocimum forskolei i benth on diabetic apoptotic and adipogenic biomarkers of diabetic rats and 3t3 l1 fibroblasts assisted by in silico approach |
| topic | <i>Ocimum forskolei</i> hesperidin streptozotocin diabetes 3T3-L1 NF-κB |
| url | https://www.mdpi.com/1420-3049/27/9/2800 |
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