Diversity Analysis and Biocontrol Potential of Cultivatable Terrestrial Bacterial <i>Streptomyces</i> in Southern China
<i>Streptomyces</i> are filamentous bacteria that are extensively present in soil, play an important role in carbon cycling, and produce a large number of highly valuable secondary metabolites. In this study, total number of 411 isolates of <i>Streptomyces</i> were collected...
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MDPI AG
2023-09-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/10/2500 |
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| author | Lifeng Zhou Fan Pei Wangling Pu Chuang Zhang Fei Chen Yuechen Hu Jie Chen Haiping Lin Xudong Zhou |
| author_facet | Lifeng Zhou Fan Pei Wangling Pu Chuang Zhang Fei Chen Yuechen Hu Jie Chen Haiping Lin Xudong Zhou |
| author_sort | Lifeng Zhou |
| collection | DOAJ |
| description | <i>Streptomyces</i> are filamentous bacteria that are extensively present in soil, play an important role in carbon cycling, and produce a large number of highly valuable secondary metabolites. In this study, total number of 411 isolates of <i>Streptomyces</i> were collected from ecologically similar habitats from 13 provinces in Southern China. They were clustered into 96 operational taxonomic units including eight potentially new species based on their 16S rDNA sequences. Phylogenetic analysis disclosed that the isolates with similar geographical distributions clustered together. Beta diversity of <i>Streptomyces</i> showed it manifests in a latitudinal diversity gradient (<i>R</i><sup>2</sup> = 0.3828, <i>p</i> = 0.0113). The beta diversity did not change significantly with geographic distance, and this could be due to the large longitudinal and relatively smaller latitudinal sampling range, as the phylogenetic clustering of regions with higher annual average temperature was analyzed with the nearest taxon index (<i>R</i> = −0.23, <i>p</i> = 0.045). Result of the <i>Streptomyces</i> biogeography evaluation shows the diversity of the genus is controlled by many of the same processes. Bioassay results disclosed that there were 27 isolates strongly antagonistic to plant pathogenic fungi and 71 isolates with strong nematocidal activity against pine wood nematode. Our results provide significant insights into the diversity and biocontrol potential of cultivatable <i>Streptomyces</i> in Southern China. |
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| language | English |
| last_indexed | 2024-03-10T21:30:33Z |
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| spelling | doaj.art-972ea0495f8f4775a87638bb782b28442023-11-19T15:21:03ZengMDPI AGAgronomy2073-43952023-09-011310250010.3390/agronomy13102500Diversity Analysis and Biocontrol Potential of Cultivatable Terrestrial Bacterial <i>Streptomyces</i> in Southern ChinaLifeng Zhou0Fan Pei1Wangling Pu2Chuang Zhang3Fei Chen4Yuechen Hu5Jie Chen6Haiping Lin7Xudong Zhou8State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, China<i>Streptomyces</i> are filamentous bacteria that are extensively present in soil, play an important role in carbon cycling, and produce a large number of highly valuable secondary metabolites. In this study, total number of 411 isolates of <i>Streptomyces</i> were collected from ecologically similar habitats from 13 provinces in Southern China. They were clustered into 96 operational taxonomic units including eight potentially new species based on their 16S rDNA sequences. Phylogenetic analysis disclosed that the isolates with similar geographical distributions clustered together. Beta diversity of <i>Streptomyces</i> showed it manifests in a latitudinal diversity gradient (<i>R</i><sup>2</sup> = 0.3828, <i>p</i> = 0.0113). The beta diversity did not change significantly with geographic distance, and this could be due to the large longitudinal and relatively smaller latitudinal sampling range, as the phylogenetic clustering of regions with higher annual average temperature was analyzed with the nearest taxon index (<i>R</i> = −0.23, <i>p</i> = 0.045). Result of the <i>Streptomyces</i> biogeography evaluation shows the diversity of the genus is controlled by many of the same processes. Bioassay results disclosed that there were 27 isolates strongly antagonistic to plant pathogenic fungi and 71 isolates with strong nematocidal activity against pine wood nematode. Our results provide significant insights into the diversity and biocontrol potential of cultivatable <i>Streptomyces</i> in Southern China.https://www.mdpi.com/2073-4395/13/10/2500Actinobacteria16S rDNAdiversitySouthern Chinabioassay |
| spellingShingle | Lifeng Zhou Fan Pei Wangling Pu Chuang Zhang Fei Chen Yuechen Hu Jie Chen Haiping Lin Xudong Zhou Diversity Analysis and Biocontrol Potential of Cultivatable Terrestrial Bacterial <i>Streptomyces</i> in Southern China Agronomy Actinobacteria 16S rDNA diversity Southern China bioassay |
| title | Diversity Analysis and Biocontrol Potential of Cultivatable Terrestrial Bacterial <i>Streptomyces</i> in Southern China |
| title_full | Diversity Analysis and Biocontrol Potential of Cultivatable Terrestrial Bacterial <i>Streptomyces</i> in Southern China |
| title_fullStr | Diversity Analysis and Biocontrol Potential of Cultivatable Terrestrial Bacterial <i>Streptomyces</i> in Southern China |
| title_full_unstemmed | Diversity Analysis and Biocontrol Potential of Cultivatable Terrestrial Bacterial <i>Streptomyces</i> in Southern China |
| title_short | Diversity Analysis and Biocontrol Potential of Cultivatable Terrestrial Bacterial <i>Streptomyces</i> in Southern China |
| title_sort | diversity analysis and biocontrol potential of cultivatable terrestrial bacterial i streptomyces i in southern china |
| topic | Actinobacteria 16S rDNA diversity Southern China bioassay |
| url | https://www.mdpi.com/2073-4395/13/10/2500 |
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