Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents
The crude methanol extracts and fractions of the root and stem barks of Dillenia serrata Thunb. showed 64% to 73% inhibition on the production of prostaglandin E2 (PGE2) in lipopolysaccharide-induced human whole blood using a radioimmunoassay technique. Three triterpenoids isolated from the root ba...
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MDPI AG
2015-02-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/20/2/3206 |
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| author | Juriyati Jalil Carla W. Sabandar Norizan Ahmat Jamia A. Jamal Ibrahim Jantan Nor-Ashila Aladdin Kartiniwati Muhammad Fhataheya Buang Hazni Falina Mohamad Idin Sahidin |
| author_facet | Juriyati Jalil Carla W. Sabandar Norizan Ahmat Jamia A. Jamal Ibrahim Jantan Nor-Ashila Aladdin Kartiniwati Muhammad Fhataheya Buang Hazni Falina Mohamad Idin Sahidin |
| author_sort | Juriyati Jalil |
| collection | DOAJ |
| description | The crude methanol extracts and fractions of the root and stem barks of Dillenia serrata Thunb. showed 64% to 73% inhibition on the production of prostaglandin E2 (PGE2) in lipopolysaccharide-induced human whole blood using a radioimmunoassay technique. Three triterpenoids isolated from the root bark of the plant, koetjapic (1), 3-oxoolean-12-en-30-oic (2), and betulinic (3) acids, exhibited significant concentration-dependent inhibitory effects on PGE2 production with IC50 values of 1.05, 1.54, and 2.59 μM, respectively, as compared with the positive control, indomethacin (IC50 = 0.45 μM). Quantification of compounds 1 and 3 in the methanol extracts and fractions were carried out by using a validated reversed-phase high performance liquid chromatography (RP-HPLC) method. The ethyl acetate fraction of the stem bark showed the highest content of both compound 1 (15.1%) and compound 3 (52.8%). The strong inhibition of the extracts and fractions on cyclooxygenase-2 (COX-2) enzymatic activity was due to the presence of their major constituents, especially koetjapic and betulinic acids. |
| first_indexed | 2024-12-10T12:08:23Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T12:08:23Z |
| publishDate | 2015-02-01 |
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| series | Molecules |
| spelling | doaj.art-972f24cb2eb6426a9549ece05ece7a3f2022-12-22T01:49:25ZengMDPI AGMolecules1420-30492015-02-012023206322010.3390/molecules20023206molecules20023206Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid ContentsJuriyati Jalil0Carla W. Sabandar1Norizan Ahmat2Jamia A. Jamal3Ibrahim Jantan4Nor-Ashila Aladdin5Kartiniwati Muhammad6Fhataheya Buang7Hazni Falina Mohamad8Idin Sahidin9Drug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, MalaysiaDrug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, MalaysiaFaculty of Applied Sciences, Universiti Teknologi Mara, Shah Alam 40450, MalaysiaDrug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, MalaysiaDrug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, MalaysiaDrug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, MalaysiaDrug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, MalaysiaDrug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, MalaysiaDrug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, MalaysiaLaboratory of Natural Products Chemistry, Faculty of Pharmacy, Halu Oleo University, Kendari 93232, IndonesiaThe crude methanol extracts and fractions of the root and stem barks of Dillenia serrata Thunb. showed 64% to 73% inhibition on the production of prostaglandin E2 (PGE2) in lipopolysaccharide-induced human whole blood using a radioimmunoassay technique. Three triterpenoids isolated from the root bark of the plant, koetjapic (1), 3-oxoolean-12-en-30-oic (2), and betulinic (3) acids, exhibited significant concentration-dependent inhibitory effects on PGE2 production with IC50 values of 1.05, 1.54, and 2.59 μM, respectively, as compared with the positive control, indomethacin (IC50 = 0.45 μM). Quantification of compounds 1 and 3 in the methanol extracts and fractions were carried out by using a validated reversed-phase high performance liquid chromatography (RP-HPLC) method. The ethyl acetate fraction of the stem bark showed the highest content of both compound 1 (15.1%) and compound 3 (52.8%). The strong inhibition of the extracts and fractions on cyclooxygenase-2 (COX-2) enzymatic activity was due to the presence of their major constituents, especially koetjapic and betulinic acids.http://www.mdpi.com/1420-3049/20/2/3206Dilleniaceae Dillenia serratatriterpenoidsprostaglandin E2HPLC |
| spellingShingle | Juriyati Jalil Carla W. Sabandar Norizan Ahmat Jamia A. Jamal Ibrahim Jantan Nor-Ashila Aladdin Kartiniwati Muhammad Fhataheya Buang Hazni Falina Mohamad Idin Sahidin Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents Molecules Dilleniaceae Dillenia serrata triterpenoids prostaglandin E2 HPLC |
| title | Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents |
| title_full | Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents |
| title_fullStr | Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents |
| title_full_unstemmed | Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents |
| title_short | Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents |
| title_sort | inhibitory effect of triterpenoids from dillenia serrata dilleniaceae on prostaglandin e2 production and quantitative hplc analysis of its koetjapic acid and betulinic acid contents |
| topic | Dilleniaceae Dillenia serrata triterpenoids prostaglandin E2 HPLC |
| url | http://www.mdpi.com/1420-3049/20/2/3206 |
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