Antiviral Defense Involves AGO4 in an Arabidopsis–Potexvirus Interaction
In plants, RNA silencing regulates gene expression through the action of Dicer-like (DCL) and Argonaute (AGO) proteins via micro RNAs and RNA-dependent DNA methylation (RdDM). In addition, RNA silencing functions as an antiviral defense mechanism by targeting virus-derived double-stranded RNA. Plant...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2016-11-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-09-16-0188-R |
| _version_ | 1818914755318906880 |
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| author | Chantal Brosseau Mohamed El Oirdi Ayooluwa Adurogbangba Xiaofang Ma Peter Moffett |
| author_facet | Chantal Brosseau Mohamed El Oirdi Ayooluwa Adurogbangba Xiaofang Ma Peter Moffett |
| author_sort | Chantal Brosseau |
| collection | DOAJ |
| description | In plants, RNA silencing regulates gene expression through the action of Dicer-like (DCL) and Argonaute (AGO) proteins via micro RNAs and RNA-dependent DNA methylation (RdDM). In addition, RNA silencing functions as an antiviral defense mechanism by targeting virus-derived double-stranded RNA. Plants encode multiple AGO proteins with specialized functions, including AGO4-like proteins that affect RdDM and AGO2, AGO5, and AGO1, which have antiviral activities. Here, we show that AGO4 is also required for defense against the potexvirus Plantago asiatica mosaic virus (PlAMV), most likely independent of RdDM components such as DCL3, Pol IV, and Pol V. Transient assays showed that AGO4 has direct antiviral activity on PlAMV and, unlike RdDM, this activity does not require nuclear localization of AGO4. Furthermore, although PlAMV infection causes a decrease in AGO4 expression, PlAMV causes a change in AGO4 localization from a largely nuclear to a largely cytoplasmic distribution. These results indicate an important role for AGO4 in targeting plant RNA viruses as well as demonstrating novel mechanisms of regulation of and by AGO4, independent of its canonical role in regulating gene expression by RdDM. |
| first_indexed | 2024-12-19T23:51:25Z |
| format | Article |
| id | doaj.art-973284e6843349719e7960229b7e24a4 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:51:25Z |
| publishDate | 2016-11-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-973284e6843349719e7960229b7e24a42022-12-21T20:01:07ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062016-11-01291187888810.1094/MPMI-09-16-0188-RAntiviral Defense Involves AGO4 in an Arabidopsis–Potexvirus InteractionChantal Brosseau0Mohamed El Oirdi1Ayooluwa Adurogbangba2Xiaofang Ma3Peter Moffett4Centre SÈVE, Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, J1K 2R1, Canada;Centre SÈVE, Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, J1K 2R1, Canada;Centre SÈVE, Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, J1K 2R1, Canada;Centre SÈVE, Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, J1K 2R1, Canada;Centre SÈVE, Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, J1K 2R1, Canada;In plants, RNA silencing regulates gene expression through the action of Dicer-like (DCL) and Argonaute (AGO) proteins via micro RNAs and RNA-dependent DNA methylation (RdDM). In addition, RNA silencing functions as an antiviral defense mechanism by targeting virus-derived double-stranded RNA. Plants encode multiple AGO proteins with specialized functions, including AGO4-like proteins that affect RdDM and AGO2, AGO5, and AGO1, which have antiviral activities. Here, we show that AGO4 is also required for defense against the potexvirus Plantago asiatica mosaic virus (PlAMV), most likely independent of RdDM components such as DCL3, Pol IV, and Pol V. Transient assays showed that AGO4 has direct antiviral activity on PlAMV and, unlike RdDM, this activity does not require nuclear localization of AGO4. Furthermore, although PlAMV infection causes a decrease in AGO4 expression, PlAMV causes a change in AGO4 localization from a largely nuclear to a largely cytoplasmic distribution. These results indicate an important role for AGO4 in targeting plant RNA viruses as well as demonstrating novel mechanisms of regulation of and by AGO4, independent of its canonical role in regulating gene expression by RdDM.https://apsjournals.apsnet.org/doi/10.1094/MPMI-09-16-0188-R |
| spellingShingle | Chantal Brosseau Mohamed El Oirdi Ayooluwa Adurogbangba Xiaofang Ma Peter Moffett Antiviral Defense Involves AGO4 in an Arabidopsis–Potexvirus Interaction Molecular Plant-Microbe Interactions |
| title | Antiviral Defense Involves AGO4 in an Arabidopsis–Potexvirus Interaction |
| title_full | Antiviral Defense Involves AGO4 in an Arabidopsis–Potexvirus Interaction |
| title_fullStr | Antiviral Defense Involves AGO4 in an Arabidopsis–Potexvirus Interaction |
| title_full_unstemmed | Antiviral Defense Involves AGO4 in an Arabidopsis–Potexvirus Interaction |
| title_short | Antiviral Defense Involves AGO4 in an Arabidopsis–Potexvirus Interaction |
| title_sort | antiviral defense involves ago4 in an arabidopsis potexvirus interaction |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-09-16-0188-R |
| work_keys_str_mv | AT chantalbrosseau antiviraldefenseinvolvesago4inanarabidopsispotexvirusinteraction AT mohamedeloirdi antiviraldefenseinvolvesago4inanarabidopsispotexvirusinteraction AT ayooluwaadurogbangba antiviraldefenseinvolvesago4inanarabidopsispotexvirusinteraction AT xiaofangma antiviraldefenseinvolvesago4inanarabidopsispotexvirusinteraction AT petermoffett antiviraldefenseinvolvesago4inanarabidopsispotexvirusinteraction |