Integrative Analysis of Metabolomics and Transcriptomics Reveals Molecular Mechanisms of Anthocyanin Metabolism in the Zikui Tea Plant (<i>Camellia sinensis</i> cv. Zikui)
In this study, we performed an association analysis of metabolomics and transcriptomics to reveal the anthocyanin biosynthesis mechanism in a new purple-leaf tea cultivar Zikui (<i>Camellia sinensis</i> cv. Zikui) (ZK). Three glycosylated anthocyanins were identified, including petunidin...
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MDPI AG
2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/9/4780 |
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| author | Ju Cai Litang Lv Xiaofang Zeng Fen Zhang Yulu Chen Weili Tian Jianrong Li Xiangyang Li Yan Li |
| author_facet | Ju Cai Litang Lv Xiaofang Zeng Fen Zhang Yulu Chen Weili Tian Jianrong Li Xiangyang Li Yan Li |
| author_sort | Ju Cai |
| collection | DOAJ |
| description | In this study, we performed an association analysis of metabolomics and transcriptomics to reveal the anthocyanin biosynthesis mechanism in a new purple-leaf tea cultivar Zikui (<i>Camellia sinensis</i> cv. Zikui) (ZK). Three glycosylated anthocyanins were identified, including petunidin 3-<i>O</i>-glucoside, cyanidin 3-<i>O</i>-galactoside, and cyanidin 3-<i>O</i>-glucoside, and their contents were the highest in ZK leaves at 15 days. This is the first report on petunidin 3-<i>O</i>-glucoside in purple-leaf tea. Integrated analysis of the transcriptome and metabolome identified eleven dependent transcription factors, among which <i>CsMYB90</i> had strong correlations with petunidin 3-<i>O</i>-glucoside, cyanidin 3-<i>O</i>-galactoside, and cyanidin 3-<i>O</i>-glucoside (PCC > 0.8). Furthermore, we also identified key correlated structural genes, including two positively correlated <i>F3’H</i> (flavonoid-3′-hydroxylase) genes, two positively correlated <i>ANS</i> (anthocyanin synthase) genes, and three negatively correlated <i>PPO</i> (polyphenol oxidase) genes. Overexpression of <i>CsMYB90</i> in tobacco resulted in dark-purple transgenic calluses. These results showed that the increased accumulation of three anthocyanins in ZK may promote purple-leaf coloration because of changes in the expression levels of genes, including <i>CsMYB90</i>, <i>F3’Hs</i>, <i>ANSs</i>, and <i>PPOs</i>. These findings reveal new insight into the molecular mechanism of anthocyanin biosynthesis in purple-leaf tea plants and provide a series of candidate genes for the breeding of anthocyanin-rich cultivars. |
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| language | English |
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| spelling | doaj.art-973294b6b2ac4eee88df0a4f4ef88cbc2023-11-23T08:22:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01239478010.3390/ijms23094780Integrative Analysis of Metabolomics and Transcriptomics Reveals Molecular Mechanisms of Anthocyanin Metabolism in the Zikui Tea Plant (<i>Camellia sinensis</i> cv. Zikui)Ju Cai0Litang Lv1Xiaofang Zeng2Fen Zhang3Yulu Chen4Weili Tian5Jianrong Li6Xiangyang Li7Yan Li8The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaCollege of Tea Sciences, Guizhou University, Guiyang 550025, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaState Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaIn this study, we performed an association analysis of metabolomics and transcriptomics to reveal the anthocyanin biosynthesis mechanism in a new purple-leaf tea cultivar Zikui (<i>Camellia sinensis</i> cv. Zikui) (ZK). Three glycosylated anthocyanins were identified, including petunidin 3-<i>O</i>-glucoside, cyanidin 3-<i>O</i>-galactoside, and cyanidin 3-<i>O</i>-glucoside, and their contents were the highest in ZK leaves at 15 days. This is the first report on petunidin 3-<i>O</i>-glucoside in purple-leaf tea. Integrated analysis of the transcriptome and metabolome identified eleven dependent transcription factors, among which <i>CsMYB90</i> had strong correlations with petunidin 3-<i>O</i>-glucoside, cyanidin 3-<i>O</i>-galactoside, and cyanidin 3-<i>O</i>-glucoside (PCC > 0.8). Furthermore, we also identified key correlated structural genes, including two positively correlated <i>F3’H</i> (flavonoid-3′-hydroxylase) genes, two positively correlated <i>ANS</i> (anthocyanin synthase) genes, and three negatively correlated <i>PPO</i> (polyphenol oxidase) genes. Overexpression of <i>CsMYB90</i> in tobacco resulted in dark-purple transgenic calluses. These results showed that the increased accumulation of three anthocyanins in ZK may promote purple-leaf coloration because of changes in the expression levels of genes, including <i>CsMYB90</i>, <i>F3’Hs</i>, <i>ANSs</i>, and <i>PPOs</i>. These findings reveal new insight into the molecular mechanism of anthocyanin biosynthesis in purple-leaf tea plants and provide a series of candidate genes for the breeding of anthocyanin-rich cultivars.https://www.mdpi.com/1422-0067/23/9/4780anthocyanin metabolismassociation analysismetabolomicspurple-leaf tea planttranscriptomics‘Zikui’ |
| spellingShingle | Ju Cai Litang Lv Xiaofang Zeng Fen Zhang Yulu Chen Weili Tian Jianrong Li Xiangyang Li Yan Li Integrative Analysis of Metabolomics and Transcriptomics Reveals Molecular Mechanisms of Anthocyanin Metabolism in the Zikui Tea Plant (<i>Camellia sinensis</i> cv. Zikui) International Journal of Molecular Sciences anthocyanin metabolism association analysis metabolomics purple-leaf tea plant transcriptomics ‘Zikui’ |
| title | Integrative Analysis of Metabolomics and Transcriptomics Reveals Molecular Mechanisms of Anthocyanin Metabolism in the Zikui Tea Plant (<i>Camellia sinensis</i> cv. Zikui) |
| title_full | Integrative Analysis of Metabolomics and Transcriptomics Reveals Molecular Mechanisms of Anthocyanin Metabolism in the Zikui Tea Plant (<i>Camellia sinensis</i> cv. Zikui) |
| title_fullStr | Integrative Analysis of Metabolomics and Transcriptomics Reveals Molecular Mechanisms of Anthocyanin Metabolism in the Zikui Tea Plant (<i>Camellia sinensis</i> cv. Zikui) |
| title_full_unstemmed | Integrative Analysis of Metabolomics and Transcriptomics Reveals Molecular Mechanisms of Anthocyanin Metabolism in the Zikui Tea Plant (<i>Camellia sinensis</i> cv. Zikui) |
| title_short | Integrative Analysis of Metabolomics and Transcriptomics Reveals Molecular Mechanisms of Anthocyanin Metabolism in the Zikui Tea Plant (<i>Camellia sinensis</i> cv. Zikui) |
| title_sort | integrative analysis of metabolomics and transcriptomics reveals molecular mechanisms of anthocyanin metabolism in the zikui tea plant i camellia sinensis i cv zikui |
| topic | anthocyanin metabolism association analysis metabolomics purple-leaf tea plant transcriptomics ‘Zikui’ |
| url | https://www.mdpi.com/1422-0067/23/9/4780 |
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