Carbon Nanotube Covalently Attached Laccase Biocathode for Biofuel Cell
Biocathode for biofuel cell was prepared by covalently immobilizedLaccaseon CNT (CNT-Laccase) using glutaraldehyde as conjugates. Successful laccase immobilization was confirmed by Fourier Transform Infrared (FTIR) Spectrophotometry, Surface Electron Microscopy (SEM) and Thermogravimetric Analysis (...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Brawijaya
2013-03-01
|
| Series: | Journal of Pure and Applied Chemistry Research |
| Subjects: | |
| Online Access: | http://www.jpacr.ub.ac.id/index.php/jpacr/article/view/143 |
| _version_ | 1831659111831306240 |
|---|---|
| author | Rizmahardian Ashari Kurniawan Aulani Aulanni'am Fa-Kuen Shieh Peter Po-Jhen Chu |
| author_facet | Rizmahardian Ashari Kurniawan Aulani Aulanni'am Fa-Kuen Shieh Peter Po-Jhen Chu |
| author_sort | Rizmahardian Ashari Kurniawan |
| collection | DOAJ |
| description | Biocathode for biofuel cell was prepared by covalently immobilizedLaccaseon CNT (CNT-Laccase) using glutaraldehyde as conjugates. Successful laccase immobilization was confirmed by Fourier Transform Infrared (FTIR) Spectrophotometry, Surface Electron Microscopy (SEM) and Thermogravimetric Analysis (TGA). Immobilization affected Laccase enzymatic activity where it boosts the stability at high temperature and neutral pH. At temperature 65ºC, free Laccase completely loss its activity, while CNT-Laccase still retaining 57.12% of its activity at 45ºC. The activity of CNT-Laccase at pH 7 was 7.04% of activity at pH 5 which was higher than that of free Laccase. CNT-Laccase was able to perform oxygen electroreduction with addition ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as mediator. Performance of oxygen electroreduction activity was also determined by type and composition of binding polymer. Nafion was able to provide better environment for oxygen electroreduction activity compare to polyvinyl alcohol (PVA). Current density resulted in using Nafion in ratio 1:10 to buffer volume was 1.31 mA/cm<sup>2</sup>, which was higher than that of PVA (1.01 mA/cm<sup>2</sup>). Increasing binding polymer ratio into 1:2 and 1:1 undermined oxygen electroreduction activity. |
| first_indexed | 2024-12-19T17:54:43Z |
| format | Article |
| id | doaj.art-973405fd61134977940dbad5f8127724 |
| institution | Directory Open Access Journal |
| issn | 2302-4690 |
| language | English |
| last_indexed | 2024-12-19T17:54:43Z |
| publishDate | 2013-03-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Pure and Applied Chemistry Research |
| spelling | doaj.art-973405fd61134977940dbad5f81277242022-12-21T20:11:51ZengUniversity of BrawijayaJournal of Pure and Applied Chemistry Research2302-46902013-03-01227988118Carbon Nanotube Covalently Attached Laccase Biocathode for Biofuel CellRizmahardian Ashari KurniawanAulani Aulanni'amFa-Kuen ShiehPeter Po-Jhen ChuBiocathode for biofuel cell was prepared by covalently immobilizedLaccaseon CNT (CNT-Laccase) using glutaraldehyde as conjugates. Successful laccase immobilization was confirmed by Fourier Transform Infrared (FTIR) Spectrophotometry, Surface Electron Microscopy (SEM) and Thermogravimetric Analysis (TGA). Immobilization affected Laccase enzymatic activity where it boosts the stability at high temperature and neutral pH. At temperature 65ºC, free Laccase completely loss its activity, while CNT-Laccase still retaining 57.12% of its activity at 45ºC. The activity of CNT-Laccase at pH 7 was 7.04% of activity at pH 5 which was higher than that of free Laccase. CNT-Laccase was able to perform oxygen electroreduction with addition ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as mediator. Performance of oxygen electroreduction activity was also determined by type and composition of binding polymer. Nafion was able to provide better environment for oxygen electroreduction activity compare to polyvinyl alcohol (PVA). Current density resulted in using Nafion in ratio 1:10 to buffer volume was 1.31 mA/cm<sup>2</sup>, which was higher than that of PVA (1.01 mA/cm<sup>2</sup>). Increasing binding polymer ratio into 1:2 and 1:1 undermined oxygen electroreduction activity.http://www.jpacr.ub.ac.id/index.php/jpacr/article/view/143Laccase, biofuel cell, biocathode, electron transfer, enzyme immobilization |
| spellingShingle | Rizmahardian Ashari Kurniawan Aulani Aulanni'am Fa-Kuen Shieh Peter Po-Jhen Chu Carbon Nanotube Covalently Attached Laccase Biocathode for Biofuel Cell Journal of Pure and Applied Chemistry Research Laccase, biofuel cell, biocathode, electron transfer, enzyme immobilization |
| title | Carbon Nanotube Covalently Attached Laccase Biocathode for Biofuel Cell |
| title_full | Carbon Nanotube Covalently Attached Laccase Biocathode for Biofuel Cell |
| title_fullStr | Carbon Nanotube Covalently Attached Laccase Biocathode for Biofuel Cell |
| title_full_unstemmed | Carbon Nanotube Covalently Attached Laccase Biocathode for Biofuel Cell |
| title_short | Carbon Nanotube Covalently Attached Laccase Biocathode for Biofuel Cell |
| title_sort | carbon nanotube covalently attached laccase biocathode for biofuel cell |
| topic | Laccase, biofuel cell, biocathode, electron transfer, enzyme immobilization |
| url | http://www.jpacr.ub.ac.id/index.php/jpacr/article/view/143 |
| work_keys_str_mv | AT rizmahardianasharikurniawan carbonnanotubecovalentlyattachedlaccasebiocathodeforbiofuelcell AT aulaniaulanniam carbonnanotubecovalentlyattachedlaccasebiocathodeforbiofuelcell AT fakuenshieh carbonnanotubecovalentlyattachedlaccasebiocathodeforbiofuelcell AT peterpojhenchu carbonnanotubecovalentlyattachedlaccasebiocathodeforbiofuelcell |