Pseudomonassin, a New Bioactive Ribosomally Synthesised and Post-Translationally Modified Peptide from <i>Pseudomonas</i> sp. SST3
Genome mining and metabolomics have become valuable tools in natural products research to evaluate and identify potential new chemistry from bacteria. In the search for new compounds from the deep-sea organism, <i>Pseudomonas</i> sp. SST3, from the South Shetland Trough, Antarctica, a co...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/10/2563 |
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| author | Kevin Jace Miranda Saif Jaber Dana Atoum Subha Arjunan Rainer Ebel Marcel Jaspars RuAngelie Edrada-Ebel |
| author_facet | Kevin Jace Miranda Saif Jaber Dana Atoum Subha Arjunan Rainer Ebel Marcel Jaspars RuAngelie Edrada-Ebel |
| author_sort | Kevin Jace Miranda |
| collection | DOAJ |
| description | Genome mining and metabolomics have become valuable tools in natural products research to evaluate and identify potential new chemistry from bacteria. In the search for new compounds from the deep-sea organism, <i>Pseudomonas</i> sp. SST3, from the South Shetland Trough, Antarctica, a co-cultivation with a second deep-sea <i>Pseudomonas zhaodongensis</i> SST2, was undertaken to isolate pseudomonassin, a ribosomally synthesised and post-translationally modified peptide (RiPP) that belongs to a class of RiPP called lasso peptides. Pseudomonassin was identified using a genome-mining approach and isolated by means of mass spectrometric guided isolation. Extensive metabolomics analysis of the co-cultivation of <i>Pseudomonas</i> sp. SST3 and <i>P. zhaodongensis</i> SST2, <i>Pseudomonas</i> sp. SST3 and <i>Escherichia coli,</i> and <i>P. zhaodongensis</i> SST2 and <i>E. coli</i> were performed using principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA), which revealed potential new metabolites in the outlier regions of the co-cultivation, with other metabolites identified previously from other species of <i>Pseudomonas.</i> The sequence of pseudomonassin was completely deduced using high collision dissociation tandem mass spectrometry (HCD-MS/MS). Preliminary studies on its activity against the pathogenic <i>P. aeruginosa</i> and its biofilm formation have been assessed and produced a minimum inhibitory concentration (MIC) of 63 μg/mL and 28 μg/mL, respectively. |
| first_indexed | 2024-03-10T21:01:47Z |
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| language | English |
| last_indexed | 2024-03-10T21:01:47Z |
| publishDate | 2023-10-01 |
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| series | Microorganisms |
| spelling | doaj.art-9738b6a4667a464aaee1cb5304d9e1f52023-11-19T17:28:40ZengMDPI AGMicroorganisms2076-26072023-10-011110256310.3390/microorganisms11102563Pseudomonassin, a New Bioactive Ribosomally Synthesised and Post-Translationally Modified Peptide from <i>Pseudomonas</i> sp. SST3Kevin Jace Miranda0Saif Jaber1Dana Atoum2Subha Arjunan3Rainer Ebel4Marcel Jaspars5RuAngelie Edrada-Ebel6Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen AB24 3UE, UKStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, John Arbuthnott Building, 161 Cathedral Street, Glasgow G4 0RE, UKDepartment of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, The Hashemite University, Zarqa 13133, JordanMarine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen AB24 3UE, UKMarine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen AB24 3UE, UKMarine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen AB24 3UE, UKStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, John Arbuthnott Building, 161 Cathedral Street, Glasgow G4 0RE, UKGenome mining and metabolomics have become valuable tools in natural products research to evaluate and identify potential new chemistry from bacteria. In the search for new compounds from the deep-sea organism, <i>Pseudomonas</i> sp. SST3, from the South Shetland Trough, Antarctica, a co-cultivation with a second deep-sea <i>Pseudomonas zhaodongensis</i> SST2, was undertaken to isolate pseudomonassin, a ribosomally synthesised and post-translationally modified peptide (RiPP) that belongs to a class of RiPP called lasso peptides. Pseudomonassin was identified using a genome-mining approach and isolated by means of mass spectrometric guided isolation. Extensive metabolomics analysis of the co-cultivation of <i>Pseudomonas</i> sp. SST3 and <i>P. zhaodongensis</i> SST2, <i>Pseudomonas</i> sp. SST3 and <i>Escherichia coli,</i> and <i>P. zhaodongensis</i> SST2 and <i>E. coli</i> were performed using principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA), which revealed potential new metabolites in the outlier regions of the co-cultivation, with other metabolites identified previously from other species of <i>Pseudomonas.</i> The sequence of pseudomonassin was completely deduced using high collision dissociation tandem mass spectrometry (HCD-MS/MS). Preliminary studies on its activity against the pathogenic <i>P. aeruginosa</i> and its biofilm formation have been assessed and produced a minimum inhibitory concentration (MIC) of 63 μg/mL and 28 μg/mL, respectively.https://www.mdpi.com/2076-2607/11/10/2563genome miningmetabolomicsribosomally synthesised and post-translationally modified peptide<i>Pseudomonas</i> sp. SST3 |
| spellingShingle | Kevin Jace Miranda Saif Jaber Dana Atoum Subha Arjunan Rainer Ebel Marcel Jaspars RuAngelie Edrada-Ebel Pseudomonassin, a New Bioactive Ribosomally Synthesised and Post-Translationally Modified Peptide from <i>Pseudomonas</i> sp. SST3 Microorganisms genome mining metabolomics ribosomally synthesised and post-translationally modified peptide <i>Pseudomonas</i> sp. SST3 |
| title | Pseudomonassin, a New Bioactive Ribosomally Synthesised and Post-Translationally Modified Peptide from <i>Pseudomonas</i> sp. SST3 |
| title_full | Pseudomonassin, a New Bioactive Ribosomally Synthesised and Post-Translationally Modified Peptide from <i>Pseudomonas</i> sp. SST3 |
| title_fullStr | Pseudomonassin, a New Bioactive Ribosomally Synthesised and Post-Translationally Modified Peptide from <i>Pseudomonas</i> sp. SST3 |
| title_full_unstemmed | Pseudomonassin, a New Bioactive Ribosomally Synthesised and Post-Translationally Modified Peptide from <i>Pseudomonas</i> sp. SST3 |
| title_short | Pseudomonassin, a New Bioactive Ribosomally Synthesised and Post-Translationally Modified Peptide from <i>Pseudomonas</i> sp. SST3 |
| title_sort | pseudomonassin a new bioactive ribosomally synthesised and post translationally modified peptide from i pseudomonas i sp sst3 |
| topic | genome mining metabolomics ribosomally synthesised and post-translationally modified peptide <i>Pseudomonas</i> sp. SST3 |
| url | https://www.mdpi.com/2076-2607/11/10/2563 |
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