FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION
Assessment of in vitro cytotoxicity as a model to explore biological activity of compounds isolated from different sources is the first approached to search molecules with potential anticancer activity. This paper is about how to implement resazurin reduction method as an indirect indicator of cell...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Universidad de Antioquia
2010-07-01
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| Series: | Vitae |
| Subjects: | |
| Online Access: | https://revistas.udea.edu.co/index.php/vitae/article/view/6342 |
| _version_ | 1818742091407163392 |
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| author | Linamaría Escobar M. Fabio A. Aristizábal G. |
| author_facet | Linamaría Escobar M. Fabio A. Aristizábal G. |
| author_sort | Linamaría Escobar M. |
| collection | DOAJ |
| description | Assessment of in vitro cytotoxicity as a model to explore biological activity of compounds isolated from different sources is the first approached to search molecules with potential anticancer activity. This paper is about how to implement resazurin reduction method as an indirect indicator of cell survival. Indeed both optimal resazurin concentration and incubation time were selected on MCF-7, MKN-45, SiHa, HEp-2, HT-29 and HeLa. Next, we compared survival results by using reduction method MTT and staining method SRB defined as end-point tests, and resazurin method described as slightly toxic to cells. The comparison of the methods was done in terms of survival rate to evaluate the sensitivity of the lines to doxorubicin HCl, a medicine used in cancer. The methods were compared in terms of percentage survival by evaluating cell line sensitivity to doxorubicin HCl (a drug used in cancer treatment). The results show that resazurin reduction method used in 4.4 μM is applicable to detect significant changes in cell population exposed to cytotoxic molecules in routine testing. |
| first_indexed | 2024-12-18T02:07:00Z |
| format | Article |
| id | doaj.art-973aa2749371435d943cd383cd09db47 |
| institution | Directory Open Access Journal |
| issn | 0121-4004 2145-2660 |
| language | English |
| last_indexed | 2024-12-18T02:07:00Z |
| publishDate | 2010-07-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Vitae |
| spelling | doaj.art-973aa2749371435d943cd383cd09db472022-12-21T21:24:34ZengUniversidad de AntioquiaVitae0121-40042145-26602010-07-01172FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATIONLinamaría Escobar M.0Fabio A. Aristizábal G.1Grupo de Farmacogenética del Cáncer. Departamento de Farmacia. Facultad de Ciencias. Universidad Nacional de Colombia.Grupo de Farmacogenética del Cáncer. Departamento de Farmacia. Facultad de Ciencias. UniveAssessment of in vitro cytotoxicity as a model to explore biological activity of compounds isolated from different sources is the first approached to search molecules with potential anticancer activity. This paper is about how to implement resazurin reduction method as an indirect indicator of cell survival. Indeed both optimal resazurin concentration and incubation time were selected on MCF-7, MKN-45, SiHa, HEp-2, HT-29 and HeLa. Next, we compared survival results by using reduction method MTT and staining method SRB defined as end-point tests, and resazurin method described as slightly toxic to cells. The comparison of the methods was done in terms of survival rate to evaluate the sensitivity of the lines to doxorubicin HCl, a medicine used in cancer. The methods were compared in terms of percentage survival by evaluating cell line sensitivity to doxorubicin HCl (a drug used in cancer treatment). The results show that resazurin reduction method used in 4.4 μM is applicable to detect significant changes in cell population exposed to cytotoxic molecules in routine testing.https://revistas.udea.edu.co/index.php/vitae/article/view/6342in vitro assaycitotoxicitycell linesresazurin |
| spellingShingle | Linamaría Escobar M. Fabio A. Aristizábal G. FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION Vitae in vitro assay citotoxicity cell lines resazurin |
| title | FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION |
| title_full | FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION |
| title_fullStr | FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION |
| title_full_unstemmed | FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION |
| title_short | FLUOROMETRIC ASSAY FOR CELL PROLIFERATION IN HUMAN TUMOR CELL LINES APPLICATION |
| title_sort | fluorometric assay for cell proliferation in human tumor cell lines application |
| topic | in vitro assay citotoxicity cell lines resazurin |
| url | https://revistas.udea.edu.co/index.php/vitae/article/view/6342 |
| work_keys_str_mv | AT linamariaescobarm fluorometricassayforcellproliferationinhumantumorcelllinesapplication AT fabioaaristizabalg fluorometricassayforcellproliferationinhumantumorcelllinesapplication |