Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis
Endothelial cells engage extracellular matrix and basement membrane components through integrin-mediated adhesion to promote angiogenesis. Angiogenesis involves the sprouting of endothelial cells from pre-existing vessels, their migration into surrounding tissue, the upregulation of angiogenesis-ass...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/5/816 |
| _version_ | 1827651746855387136 |
|---|---|
| author | Hao Xu Susan E. LaFlamme |
| author_facet | Hao Xu Susan E. LaFlamme |
| author_sort | Hao Xu |
| collection | DOAJ |
| description | Endothelial cells engage extracellular matrix and basement membrane components through integrin-mediated adhesion to promote angiogenesis. Angiogenesis involves the sprouting of endothelial cells from pre-existing vessels, their migration into surrounding tissue, the upregulation of angiogenesis-associated genes, and the formation of new endothelial tubes. To determine whether the endothelial laminin-binding integrins, α6β4, and α3β1 contribute to these processes, we employed RNAi technology in organotypic angiogenesis assays, as well in migration assays, in vitro. The endothelial depletion of either α6β4 or α3β1 inhibited endothelial sprouting, indicating that these integrins have non-redundant roles in this process. Interestingly, these phenotypes were accompanied by overlapping and distinct changes in the expression of angiogenesis-associated genes. Lastly, depletion of α6β4, but not α3β1, inhibited migration. Taken together, these results suggest that laminin-binding integrins regulate processes associated with angiogenesis by distinct and overlapping mechanisms. |
| first_indexed | 2024-03-09T20:43:54Z |
| format | Article |
| id | doaj.art-973cd16f233d43d985d4c3ed877e0f6b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T20:43:54Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-973cd16f233d43d985d4c3ed877e0f6b2023-11-23T22:50:44ZengMDPI AGCells2073-44092022-02-0111581610.3390/cells11050816Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with AngiogenesisHao Xu0Susan E. LaFlamme1Department of Regenerative and Cancer Cell Biology, Albany Medical College, 47 New Scotland Ave., Albany, NY 12208, USADepartment of Regenerative and Cancer Cell Biology, Albany Medical College, 47 New Scotland Ave., Albany, NY 12208, USAEndothelial cells engage extracellular matrix and basement membrane components through integrin-mediated adhesion to promote angiogenesis. Angiogenesis involves the sprouting of endothelial cells from pre-existing vessels, their migration into surrounding tissue, the upregulation of angiogenesis-associated genes, and the formation of new endothelial tubes. To determine whether the endothelial laminin-binding integrins, α6β4, and α3β1 contribute to these processes, we employed RNAi technology in organotypic angiogenesis assays, as well in migration assays, in vitro. The endothelial depletion of either α6β4 or α3β1 inhibited endothelial sprouting, indicating that these integrins have non-redundant roles in this process. Interestingly, these phenotypes were accompanied by overlapping and distinct changes in the expression of angiogenesis-associated genes. Lastly, depletion of α6β4, but not α3β1, inhibited migration. Taken together, these results suggest that laminin-binding integrins regulate processes associated with angiogenesis by distinct and overlapping mechanisms.https://www.mdpi.com/2073-4409/11/5/816integrinslamininangiogenesisgene expression |
| spellingShingle | Hao Xu Susan E. LaFlamme Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis Cells integrins laminin angiogenesis gene expression |
| title | Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis |
| title_full | Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis |
| title_fullStr | Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis |
| title_full_unstemmed | Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis |
| title_short | Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis |
| title_sort | contribution of endothelial laminin binding integrins to cellular processes associated with angiogenesis |
| topic | integrins laminin angiogenesis gene expression |
| url | https://www.mdpi.com/2073-4409/11/5/816 |
| work_keys_str_mv | AT haoxu contributionofendotheliallamininbindingintegrinstocellularprocessesassociatedwithangiogenesis AT susanelaflamme contributionofendotheliallamininbindingintegrinstocellularprocessesassociatedwithangiogenesis |