Plasma metabolomics reveals disrupted response and recovery following maximal exercise in myalgic encephalomyelitis/chronic fatigue syndrome
Post-exertional malaise (PEM) is a hallmark symptom of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). We monitored the evolution of 1157 plasma metabolites in 60 ME/CFS (45 female, 15 male) and 45 matched healthy control participants (30 female, 15 male) before and after 2 maximal card...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Clinical investigation
2022-05-01
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| Series: | JCI Insight |
| Subjects: | |
| Online Access: | https://doi.org/10.1172/jci.insight.157621 |
| _version_ | 1828205105003888640 |
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| author | Arnaud Germain Ludovic Giloteaux Geoffrey E. Moore Susan M. Levine John K. Chia Betsy A. Keller Jared Stevens Carl J. Franconi Xiangling Mao Dikoma C. Shungu Andrew Grimson Maureen R. Hanson |
| author_facet | Arnaud Germain Ludovic Giloteaux Geoffrey E. Moore Susan M. Levine John K. Chia Betsy A. Keller Jared Stevens Carl J. Franconi Xiangling Mao Dikoma C. Shungu Andrew Grimson Maureen R. Hanson |
| author_sort | Arnaud Germain |
| collection | DOAJ |
| description | Post-exertional malaise (PEM) is a hallmark symptom of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). We monitored the evolution of 1157 plasma metabolites in 60 ME/CFS (45 female, 15 male) and 45 matched healthy control participants (30 female, 15 male) before and after 2 maximal cardiopulmonary exercise test (CPET) challenges separated by 24 hours, with the intent of provoking PEM in patients. Four time points allowed exploration of the metabolic response to maximal energy-producing capacity and the recovery pattern of participants with ME/CFS compared with the healthy control group. Baseline comparison identified several significantly different metabolites, along with an enriched percentage of yet-to-be identified compounds. Additionally, temporal measures demonstrated an increased metabolic disparity between cohorts, including unknown metabolites. The effects of exertion in the ME/CFS cohort predominantly highlighted lipid-related as well as energy-related pathways and chemical structure clusters, which were disparately affected by the first and second exercise sessions. The 24-hour recovery period was distinct in the ME/CFS cohort, with over a quarter of the identified pathways statistically different from the controls. The pathways that are uniquely different 24 hours after an exercise challenge provide clues to metabolic disruptions that lead to PEM. Numerous altered pathways were observed to depend on glutamate metabolism, a crucial component of the homeostasis of many organs in the body, including the brain. |
| first_indexed | 2024-04-12T12:34:23Z |
| format | Article |
| id | doaj.art-973e7d0184904965933651861f8c0d1a |
| institution | Directory Open Access Journal |
| issn | 2379-3708 |
| language | English |
| last_indexed | 2024-04-12T12:34:23Z |
| publishDate | 2022-05-01 |
| publisher | American Society for Clinical investigation |
| record_format | Article |
| series | JCI Insight |
| spelling | doaj.art-973e7d0184904965933651861f8c0d1a2022-12-22T03:32:57ZengAmerican Society for Clinical investigationJCI Insight2379-37082022-05-0179Plasma metabolomics reveals disrupted response and recovery following maximal exercise in myalgic encephalomyelitis/chronic fatigue syndromeArnaud GermainLudovic GiloteauxGeoffrey E. MooreSusan M. LevineJohn K. ChiaBetsy A. KellerJared StevensCarl J. FranconiXiangling MaoDikoma C. ShunguAndrew GrimsonMaureen R. HansonPost-exertional malaise (PEM) is a hallmark symptom of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). We monitored the evolution of 1157 plasma metabolites in 60 ME/CFS (45 female, 15 male) and 45 matched healthy control participants (30 female, 15 male) before and after 2 maximal cardiopulmonary exercise test (CPET) challenges separated by 24 hours, with the intent of provoking PEM in patients. Four time points allowed exploration of the metabolic response to maximal energy-producing capacity and the recovery pattern of participants with ME/CFS compared with the healthy control group. Baseline comparison identified several significantly different metabolites, along with an enriched percentage of yet-to-be identified compounds. Additionally, temporal measures demonstrated an increased metabolic disparity between cohorts, including unknown metabolites. The effects of exertion in the ME/CFS cohort predominantly highlighted lipid-related as well as energy-related pathways and chemical structure clusters, which were disparately affected by the first and second exercise sessions. The 24-hour recovery period was distinct in the ME/CFS cohort, with over a quarter of the identified pathways statistically different from the controls. The pathways that are uniquely different 24 hours after an exercise challenge provide clues to metabolic disruptions that lead to PEM. Numerous altered pathways were observed to depend on glutamate metabolism, a crucial component of the homeostasis of many organs in the body, including the brain.https://doi.org/10.1172/jci.insight.157621Metabolism |
| spellingShingle | Arnaud Germain Ludovic Giloteaux Geoffrey E. Moore Susan M. Levine John K. Chia Betsy A. Keller Jared Stevens Carl J. Franconi Xiangling Mao Dikoma C. Shungu Andrew Grimson Maureen R. Hanson Plasma metabolomics reveals disrupted response and recovery following maximal exercise in myalgic encephalomyelitis/chronic fatigue syndrome JCI Insight Metabolism |
| title | Plasma metabolomics reveals disrupted response and recovery following maximal exercise in myalgic encephalomyelitis/chronic fatigue syndrome |
| title_full | Plasma metabolomics reveals disrupted response and recovery following maximal exercise in myalgic encephalomyelitis/chronic fatigue syndrome |
| title_fullStr | Plasma metabolomics reveals disrupted response and recovery following maximal exercise in myalgic encephalomyelitis/chronic fatigue syndrome |
| title_full_unstemmed | Plasma metabolomics reveals disrupted response and recovery following maximal exercise in myalgic encephalomyelitis/chronic fatigue syndrome |
| title_short | Plasma metabolomics reveals disrupted response and recovery following maximal exercise in myalgic encephalomyelitis/chronic fatigue syndrome |
| title_sort | plasma metabolomics reveals disrupted response and recovery following maximal exercise in myalgic encephalomyelitis chronic fatigue syndrome |
| topic | Metabolism |
| url | https://doi.org/10.1172/jci.insight.157621 |
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