Practical Approaches for Knock-Out Gene Editing in Pigs
Pigs are an important resource for meat production and serve as a model for human diseases. Due to their physiological and anatomical similarities to humans, these animals can recapitulate symptoms of human diseases, becoming an effective model for biomedical research. Although, in the past pig have...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2020.617850/full |
| _version_ | 1818368947880198144 |
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| author | Laura Daniela Ratner Laura Daniela Ratner Gaston Emilio La Motta Gaston Emilio La Motta Olinda Briski Olinda Briski Daniel Felipe Salamone Daniel Felipe Salamone Rafael Fernandez-Martin Rafael Fernandez-Martin |
| author_facet | Laura Daniela Ratner Laura Daniela Ratner Gaston Emilio La Motta Gaston Emilio La Motta Olinda Briski Olinda Briski Daniel Felipe Salamone Daniel Felipe Salamone Rafael Fernandez-Martin Rafael Fernandez-Martin |
| author_sort | Laura Daniela Ratner |
| collection | DOAJ |
| description | Pigs are an important resource for meat production and serve as a model for human diseases. Due to their physiological and anatomical similarities to humans, these animals can recapitulate symptoms of human diseases, becoming an effective model for biomedical research. Although, in the past pig have not been widely used partially because of the difficulty in genetic modification; nowadays, with the new revolutionary technology of programmable nucleases, and fundamentally of the CRISPR-Cas9 systems, it is possible for the first time to precisely modify the porcine genome as never before. To this purpose, it is necessary to introduce the system into early stage zygotes or to edit cells followed by somatic cell nuclear transfer. In this review, several strategies for pig knock-out gene editing, using the CRISPR-Cas9 system, will be summarized, as well as genotyping methods and different delivery techniques to introduce these tools into the embryos. Finally, the best approaches to produce homogeneous, biallelic edited animals will be discussed. |
| first_indexed | 2024-12-13T23:16:03Z |
| format | Article |
| id | doaj.art-9740e13b76734df88d478e25c5b4edea |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-13T23:16:03Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-9740e13b76734df88d478e25c5b4edea2022-12-21T23:27:57ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-03-011110.3389/fgene.2020.617850617850Practical Approaches for Knock-Out Gene Editing in PigsLaura Daniela Ratner0Laura Daniela Ratner1Gaston Emilio La Motta2Gaston Emilio La Motta3Olinda Briski4Olinda Briski5Daniel Felipe Salamone6Daniel Felipe Salamone7Rafael Fernandez-Martin8Rafael Fernandez-Martin9Laboratorio Biotecnología Animal (LabBA), Departamento de Producción Animal, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, ArgentinaInstituto de Investigaciones en Producción Animal (INPA), CONICET–Universidad de Buenos Aires, Buenos Aires, ArgentinaLaboratorio Biotecnología Animal (LabBA), Departamento de Producción Animal, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, ArgentinaInstituto de Investigaciones en Producción Animal (INPA), CONICET–Universidad de Buenos Aires, Buenos Aires, ArgentinaLaboratorio Biotecnología Animal (LabBA), Departamento de Producción Animal, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, ArgentinaInstituto de Investigaciones en Producción Animal (INPA), CONICET–Universidad de Buenos Aires, Buenos Aires, ArgentinaLaboratorio Biotecnología Animal (LabBA), Departamento de Producción Animal, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, ArgentinaInstituto de Investigaciones en Producción Animal (INPA), CONICET–Universidad de Buenos Aires, Buenos Aires, ArgentinaLaboratorio Biotecnología Animal (LabBA), Departamento de Producción Animal, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, ArgentinaInstituto de Investigaciones en Producción Animal (INPA), CONICET–Universidad de Buenos Aires, Buenos Aires, ArgentinaPigs are an important resource for meat production and serve as a model for human diseases. Due to their physiological and anatomical similarities to humans, these animals can recapitulate symptoms of human diseases, becoming an effective model for biomedical research. Although, in the past pig have not been widely used partially because of the difficulty in genetic modification; nowadays, with the new revolutionary technology of programmable nucleases, and fundamentally of the CRISPR-Cas9 systems, it is possible for the first time to precisely modify the porcine genome as never before. To this purpose, it is necessary to introduce the system into early stage zygotes or to edit cells followed by somatic cell nuclear transfer. In this review, several strategies for pig knock-out gene editing, using the CRISPR-Cas9 system, will be summarized, as well as genotyping methods and different delivery techniques to introduce these tools into the embryos. Finally, the best approaches to produce homogeneous, biallelic edited animals will be discussed.https://www.frontiersin.org/articles/10.3389/fgene.2020.617850/fullCRISPR-Cas9knock-outelectroporationmicroinjectionporcine zygotesSCNT |
| spellingShingle | Laura Daniela Ratner Laura Daniela Ratner Gaston Emilio La Motta Gaston Emilio La Motta Olinda Briski Olinda Briski Daniel Felipe Salamone Daniel Felipe Salamone Rafael Fernandez-Martin Rafael Fernandez-Martin Practical Approaches for Knock-Out Gene Editing in Pigs Frontiers in Genetics CRISPR-Cas9 knock-out electroporation microinjection porcine zygotes SCNT |
| title | Practical Approaches for Knock-Out Gene Editing in Pigs |
| title_full | Practical Approaches for Knock-Out Gene Editing in Pigs |
| title_fullStr | Practical Approaches for Knock-Out Gene Editing in Pigs |
| title_full_unstemmed | Practical Approaches for Knock-Out Gene Editing in Pigs |
| title_short | Practical Approaches for Knock-Out Gene Editing in Pigs |
| title_sort | practical approaches for knock out gene editing in pigs |
| topic | CRISPR-Cas9 knock-out electroporation microinjection porcine zygotes SCNT |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2020.617850/full |
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