Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between <i>Triatoma sordida</i> and <i>T. rosai</i> (Hemiptera, Triatominae)
Under laboratory conditions, <i>Triatoma rosai</i> and <i>T. sordida</i> are able to cross and produce hybrids. In the face of climate and environmental changes, the study of hybrids of triatomines has evolutionary and epidemiological implications. Therefore, we performed mor...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/11/1302 |
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| author | Roberto Dezan Vicente Fernanda Fernandez Madeira Kelly Cristine Borsatto Ariane Cristina Caris Garcia Daniel Cesaretto Cristal Luiza Maria Grzyb Delgado Isadora de Freitas Bittinelli Denis Vinicius De Mello Yago Visinho Dos Reis Amanda Ravazi Cleber Galvão Maria Tercília Vilela De Azeredo-Oliveira João Aristeu Da Rosa Jader De Oliveira Kaio Cesar Chaboli Alevi |
| author_facet | Roberto Dezan Vicente Fernanda Fernandez Madeira Kelly Cristine Borsatto Ariane Cristina Caris Garcia Daniel Cesaretto Cristal Luiza Maria Grzyb Delgado Isadora de Freitas Bittinelli Denis Vinicius De Mello Yago Visinho Dos Reis Amanda Ravazi Cleber Galvão Maria Tercília Vilela De Azeredo-Oliveira João Aristeu Da Rosa Jader De Oliveira Kaio Cesar Chaboli Alevi |
| author_sort | Roberto Dezan Vicente |
| collection | DOAJ |
| description | Under laboratory conditions, <i>Triatoma rosai</i> and <i>T. sordida</i> are able to cross and produce hybrids. In the face of climate and environmental changes, the study of hybrids of triatomines has evolutionary and epidemiological implications. Therefore, we performed morphological, cytological and molecular studies and characterized the feeding and defecation pattern of hybrids from crosses between <i>T. sordida</i> and <i>T. rosai</i>. The morphological characterization of the female genitalia of the hybrids showed that characteristics of both parental species segregated in the hybrids. Cytogenetic analyzes of hybrids showed regular metaphases. According to molecular studies, the mitochondrial marker Cytochrome B (<i>CytB</i>) related the hybrids with <i>T. sordida</i> and the nuclear marker Internal Transcribed Spacer 1 (ITS-1) related the hybrids with <i>T. rosai</i>. Both parents and hybrids defecated during the blood meal. Thus, the hybrids resulting from the cross between <i>T. sordida</i> and <i>T. rosai</i> presented segregation of phenotypic characters of both parental species, 100% homeology between homeologous chromosomes, phylogenetic relationship with <i>T sordida</i> and with <i>T. rosai</i> (with <i>CytB</i> and ITS-1, respectively), and, finally, feeding and defecation patterns similar to the parents. |
| first_indexed | 2024-03-09T18:45:13Z |
| format | Article |
| id | doaj.art-9743ce8fa2064466991aeefad1e5888e |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T18:45:13Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-9743ce8fa2064466991aeefad1e5888e2023-11-24T06:17:24ZengMDPI AGPathogens2076-08172022-11-011111130210.3390/pathogens11111302Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between <i>Triatoma sordida</i> and <i>T. rosai</i> (Hemiptera, Triatominae)Roberto Dezan Vicente0Fernanda Fernandez Madeira1Kelly Cristine Borsatto2Ariane Cristina Caris Garcia3Daniel Cesaretto Cristal4Luiza Maria Grzyb Delgado5Isadora de Freitas Bittinelli6Denis Vinicius De Mello7Yago Visinho Dos Reis8Amanda Ravazi9Cleber Galvão10Maria Tercília Vilela De Azeredo-Oliveira11João Aristeu Da Rosa12Jader De Oliveira13Kaio Cesar Chaboli Alevi14Instituto de Biociências de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu 18618-689, SP, BrazilInstituto de Biociências, Letras e Ciêcias Exatas, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), São José do Rio Preto 15054-000, SP, BrazilInstituto de Biociências, Letras e Ciêcias Exatas, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), São José do Rio Preto 15054-000, SP, BrazilInstituto de Biociências de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu 18618-689, SP, BrazilInstituto de Biociências de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu 18618-689, SP, BrazilInstituto de Biociências de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu 18618-689, SP, BrazilInstituto de Biociências de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu 18618-689, SP, BrazilInstituto de Biociências de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu 18618-689, SP, BrazilInstituto de Biociências de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu 18618-689, SP, BrazilInstituto de Biociências de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu 18618-689, SP, BrazilLaboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro 21040-360, RJ, BrazilInstituto de Biociências, Letras e Ciêcias Exatas, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), São José do Rio Preto 15054-000, SP, BrazilLaboratório de Parasitologia, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Araraquara 14801-902, SP, BrazilLaboratório de Entomologia em Saúde Pública, Faculdade de Saúde Pública, Universidade de São Paulo (USP), São Paulo 01246-904, SP, BrazilInstituto de Biociências de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu 18618-689, SP, BrazilUnder laboratory conditions, <i>Triatoma rosai</i> and <i>T. sordida</i> are able to cross and produce hybrids. In the face of climate and environmental changes, the study of hybrids of triatomines has evolutionary and epidemiological implications. Therefore, we performed morphological, cytological and molecular studies and characterized the feeding and defecation pattern of hybrids from crosses between <i>T. sordida</i> and <i>T. rosai</i>. The morphological characterization of the female genitalia of the hybrids showed that characteristics of both parental species segregated in the hybrids. Cytogenetic analyzes of hybrids showed regular metaphases. According to molecular studies, the mitochondrial marker Cytochrome B (<i>CytB</i>) related the hybrids with <i>T. sordida</i> and the nuclear marker Internal Transcribed Spacer 1 (ITS-1) related the hybrids with <i>T. rosai</i>. Both parents and hybrids defecated during the blood meal. Thus, the hybrids resulting from the cross between <i>T. sordida</i> and <i>T. rosai</i> presented segregation of phenotypic characters of both parental species, 100% homeology between homeologous chromosomes, phylogenetic relationship with <i>T sordida</i> and with <i>T. rosai</i> (with <i>CytB</i> and ITS-1, respectively), and, finally, feeding and defecation patterns similar to the parents.https://www.mdpi.com/2076-0817/11/11/1302Chagas disease vectorshybridizationmolecular biologymorphologycytogenetics |
| spellingShingle | Roberto Dezan Vicente Fernanda Fernandez Madeira Kelly Cristine Borsatto Ariane Cristina Caris Garcia Daniel Cesaretto Cristal Luiza Maria Grzyb Delgado Isadora de Freitas Bittinelli Denis Vinicius De Mello Yago Visinho Dos Reis Amanda Ravazi Cleber Galvão Maria Tercília Vilela De Azeredo-Oliveira João Aristeu Da Rosa Jader De Oliveira Kaio Cesar Chaboli Alevi Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between <i>Triatoma sordida</i> and <i>T. rosai</i> (Hemiptera, Triatominae) Pathogens Chagas disease vectors hybridization molecular biology morphology cytogenetics |
| title | Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between <i>Triatoma sordida</i> and <i>T. rosai</i> (Hemiptera, Triatominae) |
| title_full | Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between <i>Triatoma sordida</i> and <i>T. rosai</i> (Hemiptera, Triatominae) |
| title_fullStr | Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between <i>Triatoma sordida</i> and <i>T. rosai</i> (Hemiptera, Triatominae) |
| title_full_unstemmed | Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between <i>Triatoma sordida</i> and <i>T. rosai</i> (Hemiptera, Triatominae) |
| title_short | Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between <i>Triatoma sordida</i> and <i>T. rosai</i> (Hemiptera, Triatominae) |
| title_sort | morphological cytological and molecular studies and feeding and defecation pattern of hybrids from experimental crosses between i triatoma sordida i and i t rosai i hemiptera triatominae |
| topic | Chagas disease vectors hybridization molecular biology morphology cytogenetics |
| url | https://www.mdpi.com/2076-0817/11/11/1302 |
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