Seroprevalence of Toxoplasma gondii in wild boars (Sus scrofa) hunted in Ukraine
Toxoplasma gondii is an important zoonotic parasite worldwide, but it has received limited attention in Ukraine. A seroepidemiological study was conducted and samples from 452 wild boars that had been hunted in 2006–2011 in 23 of the 25 regions of Ukraine were tested to estimate T. gondii seropreval...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-04-01
|
| Series: | International Journal for Parasitology: Parasites and Wildlife |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213224423001025 |
| _version_ | 1827292731613904896 |
|---|---|
| author | Maryna Galat Gaston Moré Caroline F. Frey Ganna Kovalenko Inna Maliuk Ihor Halka Mykola Sytiuk Maksym Bezymennyi Vladyslav Galat Pikka Jokelainen |
| author_facet | Maryna Galat Gaston Moré Caroline F. Frey Ganna Kovalenko Inna Maliuk Ihor Halka Mykola Sytiuk Maksym Bezymennyi Vladyslav Galat Pikka Jokelainen |
| author_sort | Maryna Galat |
| collection | DOAJ |
| description | Toxoplasma gondii is an important zoonotic parasite worldwide, but it has received limited attention in Ukraine. A seroepidemiological study was conducted and samples from 452 wild boars that had been hunted in 2006–2011 in 23 of the 25 regions of Ukraine were tested to estimate T. gondii seroprevalence. A locally available commercial enzyme-linked immunosorbent assay (ELISA) was used for the investigation. Additionally, we tested 92 of the sera using a widely used commercial multi-species ELISA and an indirect immunofluorescence antibody test (IFAT). With the locally available ELISA, 35 of the 452 wild boars tested positive, yielding a seroprevalence estimate of 7.7% (95% confidence interval 5.5–10.5). The seropositive wild boars originated from eight of the regions. Using the majority criteria, 10/92 samples tested using both ELISAs and the IFAT were considered positive, yielding an estimated seroprevalence of 10.9% within the subset of samples. The highest seroprevalence was observed in wild boars hunted in Luhans'k (30.0%), Odesa (17.7%) and Kharkiv (12.7%). Seroprevalence was higher in older animals (13.3% for age group >12 months and 7.7% for age group ≤12 months). This is the first seroepidemiological study of T. gondii in wild boars in Ukraine. Assuming that seropositivity indicates presence of infectious parasites in the tissues, eating undercooked meat of wild boars hunted in Ukraine could be a potential source of infection to other hosts, including humans. |
| first_indexed | 2024-03-08T16:32:27Z |
| format | Article |
| id | doaj.art-97448d5250144cfe91e0fa126185ebd9 |
| institution | Directory Open Access Journal |
| issn | 2213-2244 |
| language | English |
| last_indexed | 2024-04-24T13:10:50Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal for Parasitology: Parasites and Wildlife |
| spelling | doaj.art-97448d5250144cfe91e0fa126185ebd92024-04-05T04:40:36ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442024-04-0123100901Seroprevalence of Toxoplasma gondii in wild boars (Sus scrofa) hunted in UkraineMaryna Galat0Gaston Moré1Caroline F. Frey2Ganna Kovalenko3Inna Maliuk4Ihor Halka5Mykola Sytiuk6Maksym Bezymennyi7Vladyslav Galat8Pikka Jokelainen9Institute of Parasitology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty University of Bern, Länggassstrasse 122, 3012, Bern, Switzerland; Faculty of Veterinary Medicine, National University of Life and Environmental Sciences of Ukraine, Heroiv Oborony Str. 15, 03041, Kyiv, Ukraine; Corresponding author. Institute of Parasitology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty University of Bern, Länggassstrasse 122, 3012, Bern, Switzerland.Institute of Parasitology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty University of Bern, Länggassstrasse 122, 3012, Bern, SwitzerlandInstitute of Parasitology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty University of Bern, Länggassstrasse 122, 3012, Bern, SwitzerlandInstitute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine, Donetska Str. 30, 03151, Kyiv, Ukraine; Division of Virology, Department of Pathology, University of Cambridge, Cambridge, UK; Department of Biological Sciences, University of Alaska Anchorage, Anchorage, AK, USAFaculty of Veterinary Medicine, National University of Life and Environmental Sciences of Ukraine, Heroiv Oborony Str. 15, 03041, Kyiv, UkraineInstitute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine, Donetska Str. 30, 03151, Kyiv, UkraineInstitute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine, Donetska Str. 30, 03151, Kyiv, UkraineInstitute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine, Donetska Str. 30, 03151, Kyiv, UkraineFaculty of Veterinary Medicine, National University of Life and Environmental Sciences of Ukraine, Heroiv Oborony Str. 15, 03041, Kyiv, UkraineInfectious Disease Preparedness, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen S, Denmark; Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51006, Tartu, Estonia; Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66, 00014, Helsinki, Finland; Corresponding author. Infectious Disease Preparedness, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen S, Denmark.Toxoplasma gondii is an important zoonotic parasite worldwide, but it has received limited attention in Ukraine. A seroepidemiological study was conducted and samples from 452 wild boars that had been hunted in 2006–2011 in 23 of the 25 regions of Ukraine were tested to estimate T. gondii seroprevalence. A locally available commercial enzyme-linked immunosorbent assay (ELISA) was used for the investigation. Additionally, we tested 92 of the sera using a widely used commercial multi-species ELISA and an indirect immunofluorescence antibody test (IFAT). With the locally available ELISA, 35 of the 452 wild boars tested positive, yielding a seroprevalence estimate of 7.7% (95% confidence interval 5.5–10.5). The seropositive wild boars originated from eight of the regions. Using the majority criteria, 10/92 samples tested using both ELISAs and the IFAT were considered positive, yielding an estimated seroprevalence of 10.9% within the subset of samples. The highest seroprevalence was observed in wild boars hunted in Luhans'k (30.0%), Odesa (17.7%) and Kharkiv (12.7%). Seroprevalence was higher in older animals (13.3% for age group >12 months and 7.7% for age group ≤12 months). This is the first seroepidemiological study of T. gondii in wild boars in Ukraine. Assuming that seropositivity indicates presence of infectious parasites in the tissues, eating undercooked meat of wild boars hunted in Ukraine could be a potential source of infection to other hosts, including humans.http://www.sciencedirect.com/science/article/pii/S2213224423001025AntibodiesSerologyToxoplasmosisUkraineWild boarsZoonosis |
| spellingShingle | Maryna Galat Gaston Moré Caroline F. Frey Ganna Kovalenko Inna Maliuk Ihor Halka Mykola Sytiuk Maksym Bezymennyi Vladyslav Galat Pikka Jokelainen Seroprevalence of Toxoplasma gondii in wild boars (Sus scrofa) hunted in Ukraine International Journal for Parasitology: Parasites and Wildlife Antibodies Serology Toxoplasmosis Ukraine Wild boars Zoonosis |
| title | Seroprevalence of Toxoplasma gondii in wild boars (Sus scrofa) hunted in Ukraine |
| title_full | Seroprevalence of Toxoplasma gondii in wild boars (Sus scrofa) hunted in Ukraine |
| title_fullStr | Seroprevalence of Toxoplasma gondii in wild boars (Sus scrofa) hunted in Ukraine |
| title_full_unstemmed | Seroprevalence of Toxoplasma gondii in wild boars (Sus scrofa) hunted in Ukraine |
| title_short | Seroprevalence of Toxoplasma gondii in wild boars (Sus scrofa) hunted in Ukraine |
| title_sort | seroprevalence of toxoplasma gondii in wild boars sus scrofa hunted in ukraine |
| topic | Antibodies Serology Toxoplasmosis Ukraine Wild boars Zoonosis |
| url | http://www.sciencedirect.com/science/article/pii/S2213224423001025 |
| work_keys_str_mv | AT marynagalat seroprevalenceoftoxoplasmagondiiinwildboarssusscrofahuntedinukraine AT gastonmore seroprevalenceoftoxoplasmagondiiinwildboarssusscrofahuntedinukraine AT carolineffrey seroprevalenceoftoxoplasmagondiiinwildboarssusscrofahuntedinukraine AT gannakovalenko seroprevalenceoftoxoplasmagondiiinwildboarssusscrofahuntedinukraine AT innamaliuk seroprevalenceoftoxoplasmagondiiinwildboarssusscrofahuntedinukraine AT ihorhalka seroprevalenceoftoxoplasmagondiiinwildboarssusscrofahuntedinukraine AT mykolasytiuk seroprevalenceoftoxoplasmagondiiinwildboarssusscrofahuntedinukraine AT maksymbezymennyi seroprevalenceoftoxoplasmagondiiinwildboarssusscrofahuntedinukraine AT vladyslavgalat seroprevalenceoftoxoplasmagondiiinwildboarssusscrofahuntedinukraine AT pikkajokelainen seroprevalenceoftoxoplasmagondiiinwildboarssusscrofahuntedinukraine |