Cell‐type specific analysis of translating RNAs in developing flowers reveals new levels of control
Determining both the expression levels of mRNA and the regulation of its translation is important in understanding specialized cell functions. In this study, we describe both the expression profiles of cells within spatiotemporal domains of the Arabidopsis thaliana flower and the post‐transcriptiona...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2010-01-01
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| Series: | Molecular Systems Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/msb.2010.76 |
| _version_ | 1797280735572262912 |
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| author | Yuling Jiao Elliot M Meyerowitz |
| author_facet | Yuling Jiao Elliot M Meyerowitz |
| author_sort | Yuling Jiao |
| collection | DOAJ |
| description | Determining both the expression levels of mRNA and the regulation of its translation is important in understanding specialized cell functions. In this study, we describe both the expression profiles of cells within spatiotemporal domains of the Arabidopsis thaliana flower and the post‐transcriptional regulation of these mRNAs, at nucleotide resolution. We express a tagged ribosomal protein under the promoters of three master regulators of flower development. By precipitating tagged polysomes, we isolated cell type‐specific mRNAs that are probably translating, and quantified those mRNAs through deep sequencing. Cell type comparisons identified known cell‐specific transcripts and uncovered many new ones, from which we inferred cell type‐specific hormone responses, promoter motifs and coexpressed cognate binding factor candidates, and splicing isoforms. By comparing translating mRNAs with steady‐state overall transcripts, we found evidence for widespread post‐transcriptional regulation at both the intron splicing and translational stages. Sequence analyses identified structural features associated with each step. Finally, we identified a new class of noncoding RNAs associated with polysomes. Findings from our profiling lead to new hypotheses in the understanding of flower development. |
| first_indexed | 2024-03-07T16:45:08Z |
| format | Article |
| id | doaj.art-974b62ed4a9a445a91dc49ec2641d7b5 |
| institution | Directory Open Access Journal |
| issn | 1744-4292 |
| language | English |
| last_indexed | 2024-03-07T16:45:08Z |
| publishDate | 2010-01-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj.art-974b62ed4a9a445a91dc49ec2641d7b52024-03-03T06:40:33ZengSpringer NatureMolecular Systems Biology1744-42922010-01-0161n/an/a10.1038/msb.2010.76Cell‐type specific analysis of translating RNAs in developing flowers reveals new levels of controlYuling Jiao0Elliot M Meyerowitz1Division of Biology, California Institute of Technology Pasadena CA USADivision of Biology, California Institute of Technology Pasadena CA USADetermining both the expression levels of mRNA and the regulation of its translation is important in understanding specialized cell functions. In this study, we describe both the expression profiles of cells within spatiotemporal domains of the Arabidopsis thaliana flower and the post‐transcriptional regulation of these mRNAs, at nucleotide resolution. We express a tagged ribosomal protein under the promoters of three master regulators of flower development. By precipitating tagged polysomes, we isolated cell type‐specific mRNAs that are probably translating, and quantified those mRNAs through deep sequencing. Cell type comparisons identified known cell‐specific transcripts and uncovered many new ones, from which we inferred cell type‐specific hormone responses, promoter motifs and coexpressed cognate binding factor candidates, and splicing isoforms. By comparing translating mRNAs with steady‐state overall transcripts, we found evidence for widespread post‐transcriptional regulation at both the intron splicing and translational stages. Sequence analyses identified structural features associated with each step. Finally, we identified a new class of noncoding RNAs associated with polysomes. Findings from our profiling lead to new hypotheses in the understanding of flower development.https://doi.org/10.1038/msb.2010.76Arabidopsisflowerintrontranscriptometranslation |
| spellingShingle | Yuling Jiao Elliot M Meyerowitz Cell‐type specific analysis of translating RNAs in developing flowers reveals new levels of control Molecular Systems Biology Arabidopsis flower intron transcriptome translation |
| title | Cell‐type specific analysis of translating RNAs in developing flowers reveals new levels of control |
| title_full | Cell‐type specific analysis of translating RNAs in developing flowers reveals new levels of control |
| title_fullStr | Cell‐type specific analysis of translating RNAs in developing flowers reveals new levels of control |
| title_full_unstemmed | Cell‐type specific analysis of translating RNAs in developing flowers reveals new levels of control |
| title_short | Cell‐type specific analysis of translating RNAs in developing flowers reveals new levels of control |
| title_sort | cell type specific analysis of translating rnas in developing flowers reveals new levels of control |
| topic | Arabidopsis flower intron transcriptome translation |
| url | https://doi.org/10.1038/msb.2010.76 |
| work_keys_str_mv | AT yulingjiao celltypespecificanalysisoftranslatingrnasindevelopingflowersrevealsnewlevelsofcontrol AT elliotmmeyerowitz celltypespecificanalysisoftranslatingrnasindevelopingflowersrevealsnewlevelsofcontrol |