Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats
Abstract Background Cats are susceptible to feline panleukopenia virus (FPV) and canine parvovirus (CPV) variants 2a, 2b and 2c. Detection of FPV and CPV variants in apparently healthy cats and their persistence in white blood cells (WBC) and other tissues when neutralising antibodies are simultaneo...
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | BMC Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s12917-018-1356-9 |
| _version_ | 1811272150890315776 |
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| author | Andrea Balboni Francesca Bassi Stefano De Arcangeli Rosanna Zobba Carla Dedola Alberto Alberti Mara Battilani |
| author_facet | Andrea Balboni Francesca Bassi Stefano De Arcangeli Rosanna Zobba Carla Dedola Alberto Alberti Mara Battilani |
| author_sort | Andrea Balboni |
| collection | DOAJ |
| description | Abstract Background Cats are susceptible to feline panleukopenia virus (FPV) and canine parvovirus (CPV) variants 2a, 2b and 2c. Detection of FPV and CPV variants in apparently healthy cats and their persistence in white blood cells (WBC) and other tissues when neutralising antibodies are simultaneously present, suggest that parvovirus may persist long-term in the tissues of cats post-infection without causing clinical signs. The aim of this study was to screen a population of 54 cats from Sardinia (Italy) for the presence of both FPV and CPV DNA within buffy coat samples using polymerase chain reaction (PCR). The DNA viral load, genetic diversity, phylogeny and antibody titres against parvoviruses were investigated in the positive cats. Results Carnivore protoparvovirus 1 DNA was detected in nine cats (16.7%). Viral DNA was reassembled to FPV in four cats and to CPV (CPV-2b and 2c) in four cats; one subject showed an unusually high genetic complexity with mixed infection involving FPV and CPV-2c. Antibodies against parvovirus were detected in all subjects which tested positive to DNA parvoviruses. Conclusions The identification of FPV and CPV DNA in the WBC of asymptomatic cats, despite the presence of specific antibodies against parvoviruses, and the high genetic heterogeneity detected in one sample, confirmed the relevant epidemiological role of cats in parvovirus infection. |
| first_indexed | 2024-04-12T22:35:06Z |
| format | Article |
| id | doaj.art-974c11e5adc1424596a2e70a85c2ccd0 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-12T22:35:06Z |
| publishDate | 2018-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-974c11e5adc1424596a2e70a85c2ccd02022-12-22T03:13:53ZengBMCBMC Veterinary Research1746-61482018-02-0114111010.1186/s12917-018-1356-9Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected catsAndrea Balboni0Francesca Bassi1Stefano De Arcangeli2Rosanna Zobba3Carla Dedola4Alberto Alberti5Mara Battilani6Department of Veterinary Medical Sciences, Alma Mater Studiorum - University of BolognaDepartment of Veterinary Medical Sciences, Alma Mater Studiorum - University of BolognaDepartment of Veterinary Medical Sciences, Alma Mater Studiorum - University of BolognaDepartment of Veterinary Medicine, University of SassariDepartment of Veterinary Medicine, University of SassariDepartment of Veterinary Medicine, University of SassariDepartment of Veterinary Medical Sciences, Alma Mater Studiorum - University of BolognaAbstract Background Cats are susceptible to feline panleukopenia virus (FPV) and canine parvovirus (CPV) variants 2a, 2b and 2c. Detection of FPV and CPV variants in apparently healthy cats and their persistence in white blood cells (WBC) and other tissues when neutralising antibodies are simultaneously present, suggest that parvovirus may persist long-term in the tissues of cats post-infection without causing clinical signs. The aim of this study was to screen a population of 54 cats from Sardinia (Italy) for the presence of both FPV and CPV DNA within buffy coat samples using polymerase chain reaction (PCR). The DNA viral load, genetic diversity, phylogeny and antibody titres against parvoviruses were investigated in the positive cats. Results Carnivore protoparvovirus 1 DNA was detected in nine cats (16.7%). Viral DNA was reassembled to FPV in four cats and to CPV (CPV-2b and 2c) in four cats; one subject showed an unusually high genetic complexity with mixed infection involving FPV and CPV-2c. Antibodies against parvovirus were detected in all subjects which tested positive to DNA parvoviruses. Conclusions The identification of FPV and CPV DNA in the WBC of asymptomatic cats, despite the presence of specific antibodies against parvoviruses, and the high genetic heterogeneity detected in one sample, confirmed the relevant epidemiological role of cats in parvovirus infection.http://link.springer.com/article/10.1186/s12917-018-1356-9Canine parvovirusCatCoinfectionFeline panleukopenia virusWhite blood cellsPCR |
| spellingShingle | Andrea Balboni Francesca Bassi Stefano De Arcangeli Rosanna Zobba Carla Dedola Alberto Alberti Mara Battilani Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats BMC Veterinary Research Canine parvovirus Cat Coinfection Feline panleukopenia virus White blood cells PCR |
| title | Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats |
| title_full | Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats |
| title_fullStr | Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats |
| title_full_unstemmed | Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats |
| title_short | Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats |
| title_sort | molecular analysis of carnivore protoparvovirus detected in white blood cells of naturally infected cats |
| topic | Canine parvovirus Cat Coinfection Feline panleukopenia virus White blood cells PCR |
| url | http://link.springer.com/article/10.1186/s12917-018-1356-9 |
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