Successful Intra- but Not Inter-species Recombination of msr(D) in Neisseria subflava
Resistance acquisition via natural transformation is a common process in the Neisseria genus. Transformation has played an important role in the emergence of resistance to many antimicrobials in Neisseria gonorrhoeae and Neisseria meningitidis. In a previous study, we found that currently circulatin...
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.855482/full |
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| author | Tessa de Block Natalia González Saïd Abdellati Jolein Gyonne Elise Laumen Jolein Gyonne Elise Laumen Christophe Van Dijck Christophe Van Dijck Irith De Baetselier Dorien Van den Bossche Sheeba S. Manoharan-Basil Chris Kenyon Chris Kenyon |
| author_facet | Tessa de Block Natalia González Saïd Abdellati Jolein Gyonne Elise Laumen Jolein Gyonne Elise Laumen Christophe Van Dijck Christophe Van Dijck Irith De Baetselier Dorien Van den Bossche Sheeba S. Manoharan-Basil Chris Kenyon Chris Kenyon |
| author_sort | Tessa de Block |
| collection | DOAJ |
| description | Resistance acquisition via natural transformation is a common process in the Neisseria genus. Transformation has played an important role in the emergence of resistance to many antimicrobials in Neisseria gonorrhoeae and Neisseria meningitidis. In a previous study, we found that currently circulating isolates of Neisseria subflava had acquired an msr(D) gene that has been found to result in macrolide resistance in other bacteria but never found in Neisseria species before. To determine if this resistance mechanism is transferable among Neisseria species, we assessed if we could transform the msr(D) gene into other commensal and pathogenic Neisseria under low dose azithromycin pressure. Intraspecies recombination in commensal N. subflava was confirmed with PCR and resulted in high-level macrolide resistance. Whole-genome sequencing of these transformed strains identified the complete uptake of the msr(D) integration fragment. Sequence analysis showed that a large fragment of DNA (5 and 12 kb) was transferred through a single horizontal gene transfer event. Furthermore, uptake of the msr(D) gene had no apparent fitness cost. Interspecies transformation of msr(D) from N. subflava to N. gonorrhoeae was, however, not successful. |
| first_indexed | 2024-12-13T00:50:35Z |
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| language | English |
| last_indexed | 2024-12-13T00:50:35Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-974cc81a699e465aa91a8b87d5864e132022-12-22T00:04:56ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-03-011310.3389/fmicb.2022.855482855482Successful Intra- but Not Inter-species Recombination of msr(D) in Neisseria subflavaTessa de Block0Natalia González1Saïd Abdellati2Jolein Gyonne Elise Laumen3Jolein Gyonne Elise Laumen4Christophe Van Dijck5Christophe Van Dijck6Irith De Baetselier7Dorien Van den Bossche8Sheeba S. Manoharan-Basil9Chris Kenyon10Chris Kenyon11Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, BelgiumDepartment of Clinical Sciences, Institute of Tropical Medicine, Antwerp, BelgiumDepartment of Clinical Sciences, Institute of Tropical Medicine, Antwerp, BelgiumDepartment of Clinical Sciences, Institute of Tropical Medicine, Antwerp, BelgiumLaboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, BelgiumDepartment of Clinical Sciences, Institute of Tropical Medicine, Antwerp, BelgiumLaboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, BelgiumDepartment of Clinical Sciences, Institute of Tropical Medicine, Antwerp, BelgiumDepartment of Clinical Sciences, Institute of Tropical Medicine, Antwerp, BelgiumDepartment of Clinical Sciences, Institute of Tropical Medicine, Antwerp, BelgiumDepartment of Clinical Sciences, Institute of Tropical Medicine, Antwerp, BelgiumDepartment of Medicine, University of Cape Town, Cape Town, South AfricaResistance acquisition via natural transformation is a common process in the Neisseria genus. Transformation has played an important role in the emergence of resistance to many antimicrobials in Neisseria gonorrhoeae and Neisseria meningitidis. In a previous study, we found that currently circulating isolates of Neisseria subflava had acquired an msr(D) gene that has been found to result in macrolide resistance in other bacteria but never found in Neisseria species before. To determine if this resistance mechanism is transferable among Neisseria species, we assessed if we could transform the msr(D) gene into other commensal and pathogenic Neisseria under low dose azithromycin pressure. Intraspecies recombination in commensal N. subflava was confirmed with PCR and resulted in high-level macrolide resistance. Whole-genome sequencing of these transformed strains identified the complete uptake of the msr(D) integration fragment. Sequence analysis showed that a large fragment of DNA (5 and 12 kb) was transferred through a single horizontal gene transfer event. Furthermore, uptake of the msr(D) gene had no apparent fitness cost. Interspecies transformation of msr(D) from N. subflava to N. gonorrhoeae was, however, not successful.https://www.frontiersin.org/articles/10.3389/fmicb.2022.855482/fullhorizontal gene transfermsr(D)transformationNeisseria subflavaNeisseria gonorrhoeaemacrolide resistance |
| spellingShingle | Tessa de Block Natalia González Saïd Abdellati Jolein Gyonne Elise Laumen Jolein Gyonne Elise Laumen Christophe Van Dijck Christophe Van Dijck Irith De Baetselier Dorien Van den Bossche Sheeba S. Manoharan-Basil Chris Kenyon Chris Kenyon Successful Intra- but Not Inter-species Recombination of msr(D) in Neisseria subflava Frontiers in Microbiology horizontal gene transfer msr(D) transformation Neisseria subflava Neisseria gonorrhoeae macrolide resistance |
| title | Successful Intra- but Not Inter-species Recombination of msr(D) in Neisseria subflava |
| title_full | Successful Intra- but Not Inter-species Recombination of msr(D) in Neisseria subflava |
| title_fullStr | Successful Intra- but Not Inter-species Recombination of msr(D) in Neisseria subflava |
| title_full_unstemmed | Successful Intra- but Not Inter-species Recombination of msr(D) in Neisseria subflava |
| title_short | Successful Intra- but Not Inter-species Recombination of msr(D) in Neisseria subflava |
| title_sort | successful intra but not inter species recombination of msr d in neisseria subflava |
| topic | horizontal gene transfer msr(D) transformation Neisseria subflava Neisseria gonorrhoeae macrolide resistance |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.855482/full |
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