Evaluation of extracts from Coccoloba mollis using the Salmonella/microsome system and in vivo tests
The common everyday use of medicinal plants is an ancient, and still very widespread practice, whereby the need for studies on their possible toxicity and mutagenic properties. The species Coccoloba mollis has been much used in phytotherapy, mainly in cases involving loss of memory and stress. In or...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2010-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300027 |
| _version_ | 1819168498025234432 |
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| author | Marcela Stefanini Tsuboy Juliana Cristina Marcarini Dalva Trevisan Ferreira Elisa Raquel Anastácio Ferraz Farah Maria Drumond Chequer Danielle Palma de Oliveira Lúcia Regina Ribeiro Mário Sérgio Mantovani |
| author_facet | Marcela Stefanini Tsuboy Juliana Cristina Marcarini Dalva Trevisan Ferreira Elisa Raquel Anastácio Ferraz Farah Maria Drumond Chequer Danielle Palma de Oliveira Lúcia Regina Ribeiro Mário Sérgio Mantovani |
| author_sort | Marcela Stefanini Tsuboy |
| collection | DOAJ |
| description | The common everyday use of medicinal plants is an ancient, and still very widespread practice, whereby the need for studies on their possible toxicity and mutagenic properties. The species Coccoloba mollis has been much used in phytotherapy, mainly in cases involving loss of memory and stress. In order to investigate its genotoxic and mutagenic potential, ethanolic extracts from the leaves and roots underwent Salmonella/microsome assaying (TA98 and TA100 strains, with and without exogenous metabolism - S9), besides comet and micronucleus tests in vivo.There was no significant increase in the number of revertants/plate of Salmonella strains in any of the analyzed root-extract concentrations, although the extract itself was extremely toxic to the Salmonella TA98 strain in the tests carried out with S9 (doses varying from 0.005 to 0.5 µg/plate). On the other hand, the leaf-extract induced mutations in the TA98 strain in the absence of S9 in the highest concentration evaluated, although at very low mutagenic potency (0.004 rev/µg). Furthermore, there was no statistically significant increase in the number of comets and micronuclei, in treatments involving Swiss mice. It was obvious that extracts of Coccoloba mollis, under the described experimental conditions, are not mutagenic. |
| first_indexed | 2024-12-22T19:04:33Z |
| format | Article |
| id | doaj.art-974f2f6044674a36828c89e4b33a0351 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-22T19:04:33Z |
| publishDate | 2010-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-974f2f6044674a36828c89e4b33a03512022-12-21T18:15:52ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852010-01-01333542548Evaluation of extracts from Coccoloba mollis using the Salmonella/microsome system and in vivo testsMarcela Stefanini TsuboyJuliana Cristina MarcariniDalva Trevisan FerreiraElisa Raquel Anastácio FerrazFarah Maria Drumond ChequerDanielle Palma de OliveiraLúcia Regina RibeiroMário Sérgio MantovaniThe common everyday use of medicinal plants is an ancient, and still very widespread practice, whereby the need for studies on their possible toxicity and mutagenic properties. The species Coccoloba mollis has been much used in phytotherapy, mainly in cases involving loss of memory and stress. In order to investigate its genotoxic and mutagenic potential, ethanolic extracts from the leaves and roots underwent Salmonella/microsome assaying (TA98 and TA100 strains, with and without exogenous metabolism - S9), besides comet and micronucleus tests in vivo.There was no significant increase in the number of revertants/plate of Salmonella strains in any of the analyzed root-extract concentrations, although the extract itself was extremely toxic to the Salmonella TA98 strain in the tests carried out with S9 (doses varying from 0.005 to 0.5 µg/plate). On the other hand, the leaf-extract induced mutations in the TA98 strain in the absence of S9 in the highest concentration evaluated, although at very low mutagenic potency (0.004 rev/µg). Furthermore, there was no statistically significant increase in the number of comets and micronuclei, in treatments involving Swiss mice. It was obvious that extracts of Coccoloba mollis, under the described experimental conditions, are not mutagenic.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300027medicinal plantsCoccoloba mollisAmes testin vivo comet assayin vivo micronucleus assayPolygonaceae |
| spellingShingle | Marcela Stefanini Tsuboy Juliana Cristina Marcarini Dalva Trevisan Ferreira Elisa Raquel Anastácio Ferraz Farah Maria Drumond Chequer Danielle Palma de Oliveira Lúcia Regina Ribeiro Mário Sérgio Mantovani Evaluation of extracts from Coccoloba mollis using the Salmonella/microsome system and in vivo tests Genetics and Molecular Biology medicinal plants Coccoloba mollis Ames test in vivo comet assay in vivo micronucleus assay Polygonaceae |
| title | Evaluation of extracts from Coccoloba mollis using the Salmonella/microsome system and in vivo tests |
| title_full | Evaluation of extracts from Coccoloba mollis using the Salmonella/microsome system and in vivo tests |
| title_fullStr | Evaluation of extracts from Coccoloba mollis using the Salmonella/microsome system and in vivo tests |
| title_full_unstemmed | Evaluation of extracts from Coccoloba mollis using the Salmonella/microsome system and in vivo tests |
| title_short | Evaluation of extracts from Coccoloba mollis using the Salmonella/microsome system and in vivo tests |
| title_sort | evaluation of extracts from coccoloba mollis using the salmonella microsome system and in vivo tests |
| topic | medicinal plants Coccoloba mollis Ames test in vivo comet assay in vivo micronucleus assay Polygonaceae |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300027 |
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