Cytogenetical effects of aluminum on root meristem cells of <em>Helianthus annuus</em> L.
Different aluminum concentrations (5, 50, and 100 ?M), as well as different exposure times (24, 48, and 72 h), were applied to investigate cytogenetical alterations in sunflower (Helianthus annuus L.) meristem cells. Nucleolin was also examined under aluminum stress using silver-staining method and...
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| Format: | Article |
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Sociedad Botánica de México, A. C.
2015-02-01
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| Series: | Botanical Sciences |
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| Online Access: | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/230 |
| _version_ | 1828284407426842624 |
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| author | Me Li Rong Qin Wusheng Jiang Donghua Liu |
| author_facet | Me Li Rong Qin Wusheng Jiang Donghua Liu |
| author_sort | Me Li |
| collection | DOAJ |
| description |
Different aluminum concentrations (5, 50, and 100 ?M), as well as different exposure times (24, 48, and 72 h), were applied to investigate cytogenetical alterations in sunflower (Helianthus annuus L.) meristem cells. Nucleolin was also examined under aluminum stress using silver-staining method and indirect immunofluorescent method. Results showed that the mitotic index decreased progressively when either aluminum concentration or exposure time increased. C-mitosis, anaphase bridges, and chromosome stickiness were observed in the aluminum treated root tip cells. Some particulates containing the argyrophilic NOR-associated proteins were distributed in the nucleus of the root-tip cells, and the amount of this particulate material progressively increased with increasing exposure time. Finally, the nucleolar material was extruded from the nucleus into the cytoplasm. The result also indicated the nucleolar protein nucleolin was translocated from nucleolus to nucleoplasm and cytoplasm, that presence of 50 ?M of aluminum for 48 h, suggesting that aluminum disturbed rRNA synthesis and induced the abnormal cellular location of nucleolar protein. The strongly toxic effect of aluminum on the nucleolus can inhibit or stop mitosis, and consequently inhibit root growth.
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| first_indexed | 2024-04-13T09:01:15Z |
| format | Article |
| id | doaj.art-975630412dca4166a10b5af81f74fc1f |
| institution | Directory Open Access Journal |
| issn | 2007-4298 2007-4476 |
| language | English |
| last_indexed | 2024-04-13T09:01:15Z |
| publishDate | 2015-02-01 |
| publisher | Sociedad Botánica de México, A. C. |
| record_format | Article |
| series | Botanical Sciences |
| spelling | doaj.art-975630412dca4166a10b5af81f74fc1f2022-12-22T02:53:08ZengSociedad Botánica de México, A. C.Botanical Sciences2007-42982007-44762015-02-0193110.17129/botsci.230Cytogenetical effects of aluminum on root meristem cells of <em>Helianthus annuus</em> L.Me Li0Rong Qin1Wusheng Jiang2Donghua Liu3Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin, China -and- Renhuai No.4 Middle School, Renhuai City, Guizhou Province, China Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin, China and College of Life Sciences, South China Normal University, Guangzhou, ChinaTianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin, ChinaTianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin, China Different aluminum concentrations (5, 50, and 100 ?M), as well as different exposure times (24, 48, and 72 h), were applied to investigate cytogenetical alterations in sunflower (Helianthus annuus L.) meristem cells. Nucleolin was also examined under aluminum stress using silver-staining method and indirect immunofluorescent method. Results showed that the mitotic index decreased progressively when either aluminum concentration or exposure time increased. C-mitosis, anaphase bridges, and chromosome stickiness were observed in the aluminum treated root tip cells. Some particulates containing the argyrophilic NOR-associated proteins were distributed in the nucleus of the root-tip cells, and the amount of this particulate material progressively increased with increasing exposure time. Finally, the nucleolar material was extruded from the nucleus into the cytoplasm. The result also indicated the nucleolar protein nucleolin was translocated from nucleolus to nucleoplasm and cytoplasm, that presence of 50 ?M of aluminum for 48 h, suggesting that aluminum disturbed rRNA synthesis and induced the abnormal cellular location of nucleolar protein. The strongly toxic effect of aluminum on the nucleolus can inhibit or stop mitosis, and consequently inhibit root growth. https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/230aluminumcytogenetical alterationsmitosisnucleolinsunflower |
| spellingShingle | Me Li Rong Qin Wusheng Jiang Donghua Liu Cytogenetical effects of aluminum on root meristem cells of <em>Helianthus annuus</em> L. Botanical Sciences aluminum cytogenetical alterations mitosis nucleolin sunflower |
| title | Cytogenetical effects of aluminum on root meristem cells of <em>Helianthus annuus</em> L. |
| title_full | Cytogenetical effects of aluminum on root meristem cells of <em>Helianthus annuus</em> L. |
| title_fullStr | Cytogenetical effects of aluminum on root meristem cells of <em>Helianthus annuus</em> L. |
| title_full_unstemmed | Cytogenetical effects of aluminum on root meristem cells of <em>Helianthus annuus</em> L. |
| title_short | Cytogenetical effects of aluminum on root meristem cells of <em>Helianthus annuus</em> L. |
| title_sort | cytogenetical effects of aluminum on root meristem cells of em helianthus annuus em l |
| topic | aluminum cytogenetical alterations mitosis nucleolin sunflower |
| url | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/230 |
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