HPLC-DAD Analysis and Investigation of Biological Properties of the Leaves of <i>Globularia alypum</i> (L.), Infusion Extract
<i>Globularia alypum</i> L. (GA) belonging to the Globulariaceae family is a Mediterranean plant which is widely used in traditional Tunisian medicine. The aim of this study was to investigate the phytochemical composition, antioxidant, anti-arthritic, antiproliferative, antibacterial an...
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MDPI AG
2023-12-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/12/1726 |
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| author | Sahar Nouir Aicha Laouani Ahmed Loghmari Khaireddine Bouassida Raoudha Slim Rim Bouhajeb Yosra Hasni Khaled Saguem Bouraoui Ouni Amira Zaïri |
| author_facet | Sahar Nouir Aicha Laouani Ahmed Loghmari Khaireddine Bouassida Raoudha Slim Rim Bouhajeb Yosra Hasni Khaled Saguem Bouraoui Ouni Amira Zaïri |
| author_sort | Sahar Nouir |
| collection | DOAJ |
| description | <i>Globularia alypum</i> L. (GA) belonging to the Globulariaceae family is a Mediterranean plant which is widely used in traditional Tunisian medicine. The aim of this study was to investigate the phytochemical composition, antioxidant, anti-arthritic, antiproliferative, antibacterial and antibiofilm potential of aqueous GA leaf extracts (AGAL). Quantitative analyses of the different constituents of extracts were evaluated by high-performance liquid chromatography with photodiode-array detection (HPLC-DAD). Spectrophotometric methods and chemical tests were used for antioxidant and anti-arthritic activities. The antiproliferative study was evaluated using colorectal cancer SW620 cells, while the antibacterial assessment and analysis of the antibiofilm effects were determined by the microdilution method and the crystal violet assay, respectively. AGAL extracts presented several components, mainly Nepetin-7-Glucoside and trans-ferrulic acid. The results showed that they had an important antioxidant (IC<sub>50</sub> = 0.34; 0.38 and 1.20 mg/mL) and anti-arthritic (IC<sub>50</sub> = 2.94 mg/mL) properties, and these effects are displayed in a dose-dependent manner. In addition, this extract demonstrated significant antiproliferative (IC<sub>50</sub> = 50 µg/mL), antibacterial (MIC = 6.25 mg/mL and MBC = 6.25 mg/mL), and antibiofilm (59.70% at 25 mg/mL) properties especially against <i>S. aureus</i>. The results achieved confirm the important role of this plant as a source of therapeutic activities. |
| first_indexed | 2024-03-08T20:27:09Z |
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| language | English |
| last_indexed | 2024-03-08T20:27:09Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-975b4d0a28274826b4c2e1073ca7215e2023-12-22T14:31:42ZengMDPI AGPharmaceuticals1424-82472023-12-011612172610.3390/ph16121726HPLC-DAD Analysis and Investigation of Biological Properties of the Leaves of <i>Globularia alypum</i> (L.), Infusion ExtractSahar Nouir0Aicha Laouani1Ahmed Loghmari2Khaireddine Bouassida3Raoudha Slim4Rim Bouhajeb5Yosra Hasni6Khaled Saguem7Bouraoui Ouni8Amira Zaïri9Laboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, TunisiaLaboratory of Metabolic Biophysics and Applied Pharmacology (LR12/ES02), Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, TunisiaUrology Department, Sahloul Hospital, Sousse 4054, TunisiaUrology Department, Sahloul Hospital, Sousse 4054, TunisiaLaboratory of Bioactive Natural Substances and Biotechnology Research, Faculty of Dental Medicine of Monastir, University of Monastir, Sousse 4023, TunisiaDepartment of Pharmacology, Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, TunisiaDepartment of Endocrinology, Farhat Hached Hospital of Sousse, Sousse 4000, TunisiaLaboratory of Metabolic Biophysics and Applied Pharmacology (LR12/ES02), Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, TunisiaLaboratory of Bioactive Natural Substances and Biotechnology Research, Faculty of Dental Medicine of Monastir, University of Monastir, Sousse 4023, TunisiaLaboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4023, Tunisia<i>Globularia alypum</i> L. (GA) belonging to the Globulariaceae family is a Mediterranean plant which is widely used in traditional Tunisian medicine. The aim of this study was to investigate the phytochemical composition, antioxidant, anti-arthritic, antiproliferative, antibacterial and antibiofilm potential of aqueous GA leaf extracts (AGAL). Quantitative analyses of the different constituents of extracts were evaluated by high-performance liquid chromatography with photodiode-array detection (HPLC-DAD). Spectrophotometric methods and chemical tests were used for antioxidant and anti-arthritic activities. The antiproliferative study was evaluated using colorectal cancer SW620 cells, while the antibacterial assessment and analysis of the antibiofilm effects were determined by the microdilution method and the crystal violet assay, respectively. AGAL extracts presented several components, mainly Nepetin-7-Glucoside and trans-ferrulic acid. The results showed that they had an important antioxidant (IC<sub>50</sub> = 0.34; 0.38 and 1.20 mg/mL) and anti-arthritic (IC<sub>50</sub> = 2.94 mg/mL) properties, and these effects are displayed in a dose-dependent manner. In addition, this extract demonstrated significant antiproliferative (IC<sub>50</sub> = 50 µg/mL), antibacterial (MIC = 6.25 mg/mL and MBC = 6.25 mg/mL), and antibiofilm (59.70% at 25 mg/mL) properties especially against <i>S. aureus</i>. The results achieved confirm the important role of this plant as a source of therapeutic activities.https://www.mdpi.com/1424-8247/16/12/1726<i>Globularia alypum</i> L.HPLC-DADantioxidant activityanti-arthritic activityantiproliferative effectantibacterial and antibiofilm activities |
| spellingShingle | Sahar Nouir Aicha Laouani Ahmed Loghmari Khaireddine Bouassida Raoudha Slim Rim Bouhajeb Yosra Hasni Khaled Saguem Bouraoui Ouni Amira Zaïri HPLC-DAD Analysis and Investigation of Biological Properties of the Leaves of <i>Globularia alypum</i> (L.), Infusion Extract Pharmaceuticals <i>Globularia alypum</i> L. HPLC-DAD antioxidant activity anti-arthritic activity antiproliferative effect antibacterial and antibiofilm activities |
| title | HPLC-DAD Analysis and Investigation of Biological Properties of the Leaves of <i>Globularia alypum</i> (L.), Infusion Extract |
| title_full | HPLC-DAD Analysis and Investigation of Biological Properties of the Leaves of <i>Globularia alypum</i> (L.), Infusion Extract |
| title_fullStr | HPLC-DAD Analysis and Investigation of Biological Properties of the Leaves of <i>Globularia alypum</i> (L.), Infusion Extract |
| title_full_unstemmed | HPLC-DAD Analysis and Investigation of Biological Properties of the Leaves of <i>Globularia alypum</i> (L.), Infusion Extract |
| title_short | HPLC-DAD Analysis and Investigation of Biological Properties of the Leaves of <i>Globularia alypum</i> (L.), Infusion Extract |
| title_sort | hplc dad analysis and investigation of biological properties of the leaves of i globularia alypum i l infusion extract |
| topic | <i>Globularia alypum</i> L. HPLC-DAD antioxidant activity anti-arthritic activity antiproliferative effect antibacterial and antibiofilm activities |
| url | https://www.mdpi.com/1424-8247/16/12/1726 |
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