Rapid Identification of Emerging Pathogens: Coronavirus
We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrome...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Centers for Disease Control and Prevention
2005-03-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/11/3/04-0629_article |
| _version_ | 1818286621842210816 |
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| author | Rangarajan Sampath Steven A. Hofstadler Lawrence B. Blyn Mark W. Eshoo Thomas A. Hall Christian Massire Harold M. Levene James C. Hannis Patina M. Harrell Benjamin Neuman Michael J. Buchmeier Yun Jiang Raymond Ranken Jared J. Drader Vivek Samant Richard H. Griffey John A. McNeil Stanley T. Crooke David J. Ecker |
| author_facet | Rangarajan Sampath Steven A. Hofstadler Lawrence B. Blyn Mark W. Eshoo Thomas A. Hall Christian Massire Harold M. Levene James C. Hannis Patina M. Harrell Benjamin Neuman Michael J. Buchmeier Yun Jiang Raymond Ranken Jared J. Drader Vivek Samant Richard H. Griffey John A. McNeil Stanley T. Crooke David J. Ecker |
| author_sort | Rangarajan Sampath |
| collection | DOAJ |
| description | We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to identify organisms in a sample. We demonstrate this principle by using 14 isolates of 9 diverse Coronavirus spp., including the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). We show that this method could identify and distinguish between SARS and other known CoV, including the human CoV 229E and OC43, individually and in a mixture of all 3 human viruses. The sensitivity of detection, measured by using titered SARS-CoV spiked into human serum, was ≈1 PFU/mL. This approach, applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens, is capable of automated analysis of >900 PCR reactions per day. |
| first_indexed | 2024-12-13T01:27:31Z |
| format | Article |
| id | doaj.art-975f81ff9d7747e9b07b9e7a00d3a9dd |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-13T01:27:31Z |
| publishDate | 2005-03-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-975f81ff9d7747e9b07b9e7a00d3a9dd2022-12-22T00:04:05ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592005-03-0111337337910.3201/eid1103.040629Rapid Identification of Emerging Pathogens: CoronavirusRangarajan SampathSteven A. HofstadlerLawrence B. BlynMark W. EshooThomas A. HallChristian MassireHarold M. LeveneJames C. HannisPatina M. HarrellBenjamin NeumanMichael J. BuchmeierYun JiangRaymond RankenJared J. DraderVivek SamantRichard H. GriffeyJohn A. McNeilStanley T. CrookeDavid J. EckerWe describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to identify organisms in a sample. We demonstrate this principle by using 14 isolates of 9 diverse Coronavirus spp., including the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). We show that this method could identify and distinguish between SARS and other known CoV, including the human CoV 229E and OC43, individually and in a mixture of all 3 human viruses. The sensitivity of detection, measured by using titered SARS-CoV spiked into human serum, was ≈1 PFU/mL. This approach, applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens, is capable of automated analysis of >900 PCR reactions per day.https://wwwnc.cdc.gov/eid/article/11/3/04-0629_articlePCRmolecular epidemiologyemerging pathogensSARS virusinfectious disease surveillanceESI mass spectrometry |
| spellingShingle | Rangarajan Sampath Steven A. Hofstadler Lawrence B. Blyn Mark W. Eshoo Thomas A. Hall Christian Massire Harold M. Levene James C. Hannis Patina M. Harrell Benjamin Neuman Michael J. Buchmeier Yun Jiang Raymond Ranken Jared J. Drader Vivek Samant Richard H. Griffey John A. McNeil Stanley T. Crooke David J. Ecker Rapid Identification of Emerging Pathogens: Coronavirus Emerging Infectious Diseases PCR molecular epidemiology emerging pathogens SARS virus infectious disease surveillance ESI mass spectrometry |
| title | Rapid Identification of Emerging Pathogens: Coronavirus |
| title_full | Rapid Identification of Emerging Pathogens: Coronavirus |
| title_fullStr | Rapid Identification of Emerging Pathogens: Coronavirus |
| title_full_unstemmed | Rapid Identification of Emerging Pathogens: Coronavirus |
| title_short | Rapid Identification of Emerging Pathogens: Coronavirus |
| title_sort | rapid identification of emerging pathogens coronavirus |
| topic | PCR molecular epidemiology emerging pathogens SARS virus infectious disease surveillance ESI mass spectrometry |
| url | https://wwwnc.cdc.gov/eid/article/11/3/04-0629_article |
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