Study models for inducing tumors by 7,12-dimethylbenz (A) - Antracene in rat mammary gland
Five study models (A, B, C, D, E) were used to investigate the effect of 7, 12-dimethylbenz(a)anthracene in inducing tumors in the mammary tissue of Spreague-Dawiey rats. Each group was subjected ad libitum to the foiiowing diets: group A - commercial ration; groups B/C - a semipurified linoleic ac...
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| Language: | English |
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Instituto Nacional de Câncer (INCA)
2023-07-01
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| Series: | Revista Brasileira de Cancerologia |
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| Online Access: | https://rbc.inca.gov.br/index.php/revista/article/view/3103 |
| _version_ | 1797280260055629824 |
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| author | Fernando Guimarães Quivo Schwartzburd Tahin Tereza Cristina Cavalcanti Clara Pérola Baumel |
| author_facet | Fernando Guimarães Quivo Schwartzburd Tahin Tereza Cristina Cavalcanti Clara Pérola Baumel |
| author_sort | Fernando Guimarães |
| collection | DOAJ |
| description |
Five study models (A, B, C, D, E) were used to investigate the effect of 7, 12-dimethylbenz(a)anthracene in inducing tumors in the mammary tissue of Spreague-Dawiey rats. Each group was subjected ad libitum to the foiiowing diets: group A - commercial ration; groups B/C - a semipurified linoleic acid-rích diet; group D - a semipurified saturated fatty acid-rich diet; group E - a semipurified fat diet composed of 50% soybean 0ff (rich in essential fatty acid), 25% olive oil (rich in oleic acid) and 25% coconut oil (rich in saturated fatty acid). The animais were fed through intragastric instiiation, one or two doses of DMBA dissolved in 1 ml of soybean oil Group A animais received a single 10 mg-DMBA-doses 60 days after birth. Group 8 animals received two-20 mg-DMBA -doses, the first dose 45 days after birth and the second 60 days after birth. Groups C, D and E animais were treated in the same way as group 8 animais. The control-groups were fed 1 mi-piacebo-soybean oiL DMBA induced tumors in the treated rats as visualized in the following results: 1) Groups A, B, C, O and E developed 52%, 76%, 88%, 60% and 67% tumors, respectively; 2) in the test groups A, B, C, D and E the first paipabie tumor was reported at 200 ± 52, 103 ± 36, 48 ± 1, 133 ± 62 and 183 ± 45 days after DMBA administration, respectively. 3) 14.8%, 40.7%, 97.5%, 50.0% and 41.7% of all tumors were classified as adenocarcinoma.The results suggest that study model "C" was the most effective one, inducing higher incidence of adenocarcinomas in DMBA-treated-rats, the symptons being evidenced in the shortest experimental time.
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| first_indexed | 2024-03-07T16:38:32Z |
| format | Article |
| id | doaj.art-975f9bd749904adcb580554f9b4b3fbf |
| institution | Directory Open Access Journal |
| issn | 2176-9745 |
| language | English |
| last_indexed | 2024-03-07T16:38:32Z |
| publishDate | 2023-07-01 |
| publisher | Instituto Nacional de Câncer (INCA) |
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| series | Revista Brasileira de Cancerologia |
| spelling | doaj.art-975f9bd749904adcb580554f9b4b3fbf2024-03-03T09:24:56ZengInstituto Nacional de Câncer (INCA)Revista Brasileira de Cancerologia2176-97452023-07-01371/410.32635/2176-9745.RBC.1991v37n1/4.3103Study models for inducing tumors by 7,12-dimethylbenz (A) - Antracene in rat mammary glandFernando Guimarães0Quivo Schwartzburd Tahin1Tereza Cristina Cavalcanti2Clara Pérola Baumel3Centro de Atenção Integral à Saúde da Mulher (CAISM). Universidade Estadual de Campinas (Unicamp). Campinas (SP), BrasilCentro de Atenção Integral à Saúde da Mulher (CAISM). Universidade Estadual de Campinas (Unicamp). Campinas (SP), BrasilCentro de Atenção Integral à Saúde da Mulher (CAISM). Universidade Estadual de Campinas (Unicamp). Campinas (SP), BrasilCentro de Atenção Integral à Saúde da Mulher (CAISM). Universidade Estadual de Campinas (Unicamp). Campinas (SP), Brasil Five study models (A, B, C, D, E) were used to investigate the effect of 7, 12-dimethylbenz(a)anthracene in inducing tumors in the mammary tissue of Spreague-Dawiey rats. Each group was subjected ad libitum to the foiiowing diets: group A - commercial ration; groups B/C - a semipurified linoleic acid-rích diet; group D - a semipurified saturated fatty acid-rich diet; group E - a semipurified fat diet composed of 50% soybean 0ff (rich in essential fatty acid), 25% olive oil (rich in oleic acid) and 25% coconut oil (rich in saturated fatty acid). The animais were fed through intragastric instiiation, one or two doses of DMBA dissolved in 1 ml of soybean oil Group A animais received a single 10 mg-DMBA-doses 60 days after birth. Group 8 animals received two-20 mg-DMBA -doses, the first dose 45 days after birth and the second 60 days after birth. Groups C, D and E animais were treated in the same way as group 8 animais. The control-groups were fed 1 mi-piacebo-soybean oiL DMBA induced tumors in the treated rats as visualized in the following results: 1) Groups A, B, C, O and E developed 52%, 76%, 88%, 60% and 67% tumors, respectively; 2) in the test groups A, B, C, D and E the first paipabie tumor was reported at 200 ± 52, 103 ± 36, 48 ± 1, 133 ± 62 and 183 ± 45 days after DMBA administration, respectively. 3) 14.8%, 40.7%, 97.5%, 50.0% and 41.7% of all tumors were classified as adenocarcinoma.The results suggest that study model "C" was the most effective one, inducing higher incidence of adenocarcinomas in DMBA-treated-rats, the symptons being evidenced in the shortest experimental time. https://rbc.inca.gov.br/index.php/revista/article/view/3103Breast CancerDMBArat mammary glandstudy modeis |
| spellingShingle | Fernando Guimarães Quivo Schwartzburd Tahin Tereza Cristina Cavalcanti Clara Pérola Baumel Study models for inducing tumors by 7,12-dimethylbenz (A) - Antracene in rat mammary gland Revista Brasileira de Cancerologia Breast Cancer DMBA rat mammary gland study modeis |
| title | Study models for inducing tumors by 7,12-dimethylbenz (A) - Antracene in rat mammary gland |
| title_full | Study models for inducing tumors by 7,12-dimethylbenz (A) - Antracene in rat mammary gland |
| title_fullStr | Study models for inducing tumors by 7,12-dimethylbenz (A) - Antracene in rat mammary gland |
| title_full_unstemmed | Study models for inducing tumors by 7,12-dimethylbenz (A) - Antracene in rat mammary gland |
| title_short | Study models for inducing tumors by 7,12-dimethylbenz (A) - Antracene in rat mammary gland |
| title_sort | study models for inducing tumors by 7 12 dimethylbenz a antracene in rat mammary gland |
| topic | Breast Cancer DMBA rat mammary gland study modeis |
| url | https://rbc.inca.gov.br/index.php/revista/article/view/3103 |
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