RETRACTED ARTICLE: Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity
Abstract Background Mitrella kentii (M. kentii) (Bl.) Miq, is a tree-climbing liana that belongs to the family Annonaceae. The plant is rich with isoquinoline alkaloids, terpenylated dihydrochalcones and benzoic acids and has been reported to possess anti-inflammatory activity. The purpose of this s...
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| Format: | Article |
| Language: | English |
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BMC
2013-07-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | https://doi.org/10.1186/1472-6882-13-183 |
| _version_ | 1797199686738640896 |
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| author | Heyam Mohamed Ali Sidahmed Ainnul Hamidah Syahadah Azizan Syam Mohan Mahmood Ameen Abdulla Siddig Ibrahim Abdelwahab Manal Mohamed Elhassan Taha A Hamid A Hadi Kamal Aziz Ketuly Najihah Mohd Hashim Mun Fai Loke Jamuna Vadivelu |
| author_facet | Heyam Mohamed Ali Sidahmed Ainnul Hamidah Syahadah Azizan Syam Mohan Mahmood Ameen Abdulla Siddig Ibrahim Abdelwahab Manal Mohamed Elhassan Taha A Hamid A Hadi Kamal Aziz Ketuly Najihah Mohd Hashim Mun Fai Loke Jamuna Vadivelu |
| author_sort | Heyam Mohamed Ali Sidahmed |
| collection | DOAJ |
| description | Abstract Background Mitrella kentii (M. kentii) (Bl.) Miq, is a tree-climbing liana that belongs to the family Annonaceae. The plant is rich with isoquinoline alkaloids, terpenylated dihydrochalcones and benzoic acids and has been reported to possess anti-inflammatory activity. The purpose of this study is to assess the gastroprotective effects of desmosdumotin C (DES), a new isolated bioactive compound from M. kentii, on gastric ulcer models in rats. Methods DES was isolated from the bark of M. kentii. Experimental rats were orally pretreated with 5, 10 and 20 mg/kg of the isolated compound and were subsequently subjected to absolute ethanol-induced acute gastric ulcer. Gross evaluation, mucus content, gastric acidity and histological gastric lesions were assessed in vivo. The effects of DES on the anti-oxidant system, non-protein sulfhydryl (NP-SH) content, nitric oxide (NO)level, cyclooxygenase-2 (COX-2) enzyme activity, bcl-2-associated X (Bax) protein expression and Helicabacter pylori (H pylori) were also investigated. Results DES pre-treatment at the administered doses significantly attenuated ethanol-induced gastric ulcer; this was observed by decreased gastric ulcer area, reduced or absence of edema and leucocytes infiltration compared to the ulcer control group. It was found that DES maintained glutathione (GSH) level, decreased malondialdehyde (MDA) level, increased NP-SH content and NO level and inhibited COX-2 activity. The compound up regulated heat shock protein-70 (HSP-70) and down regulated Bax protein expression in the ulcerated tissue. DES showed interesting anti-H pylori effects. The efficacy of DES was accomplished safely without any signs of toxicity. Conclusions The current study reveals that DES demonstrated gastroprotective effects which could be attributed to its antioxidant effect, activation of HSP-70 protein, intervention with COX-2 inflammatory pathway and potent anti H pylori effect. |
| first_indexed | 2024-04-24T07:19:42Z |
| format | Article |
| id | doaj.art-97631cc7582848eea0757d85b7c981d1 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-04-24T07:19:42Z |
| publishDate | 2013-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-97631cc7582848eea0757d85b7c981d12024-04-21T11:08:25ZengBMCBMC Complementary and Alternative Medicine1472-68822013-07-0113111510.1186/1472-6882-13-183RETRACTED ARTICLE: Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activityHeyam Mohamed Ali Sidahmed0Ainnul Hamidah Syahadah Azizan1Syam Mohan2Mahmood Ameen Abdulla3Siddig Ibrahim Abdelwahab4Manal Mohamed Elhassan Taha5A Hamid A Hadi6Kamal Aziz Ketuly7Najihah Mohd Hashim8Mun Fai Loke9Jamuna Vadivelu10Department of Pharmacy, Faculty of Medicine, University of MalayaDepartment of Chemistry, Faculty of Science, University of MalayaDepartment of Pharmacy, Faculty of Medicine, University of MalayaDepartment of Molecular Medicine, Faculty of Medicine, University of MalayaMedical Research Centre, Jazan UniversityMedical Research Centre, Jazan UniversityDepartment of Chemistry, Faculty of Science, University of MalayaDepartment of Chemistry, Faculty of Science, University of MalayaDepartment of Pharmacy, Faculty of Medicine, University of MalayaDepartment of Medical Microbiology, Faculty of Medicine, University of MalayaDepartment of Medical Microbiology, Faculty of Medicine, University of MalayaAbstract Background Mitrella kentii (M. kentii) (Bl.) Miq, is a tree-climbing liana that belongs to the family Annonaceae. The plant is rich with isoquinoline alkaloids, terpenylated dihydrochalcones and benzoic acids and has been reported to possess anti-inflammatory activity. The purpose of this study is to assess the gastroprotective effects of desmosdumotin C (DES), a new isolated bioactive compound from M. kentii, on gastric ulcer models in rats. Methods DES was isolated from the bark of M. kentii. Experimental rats were orally pretreated with 5, 10 and 20 mg/kg of the isolated compound and were subsequently subjected to absolute ethanol-induced acute gastric ulcer. Gross evaluation, mucus content, gastric acidity and histological gastric lesions were assessed in vivo. The effects of DES on the anti-oxidant system, non-protein sulfhydryl (NP-SH) content, nitric oxide (NO)level, cyclooxygenase-2 (COX-2) enzyme activity, bcl-2-associated X (Bax) protein expression and Helicabacter pylori (H pylori) were also investigated. Results DES pre-treatment at the administered doses significantly attenuated ethanol-induced gastric ulcer; this was observed by decreased gastric ulcer area, reduced or absence of edema and leucocytes infiltration compared to the ulcer control group. It was found that DES maintained glutathione (GSH) level, decreased malondialdehyde (MDA) level, increased NP-SH content and NO level and inhibited COX-2 activity. The compound up regulated heat shock protein-70 (HSP-70) and down regulated Bax protein expression in the ulcerated tissue. DES showed interesting anti-H pylori effects. The efficacy of DES was accomplished safely without any signs of toxicity. Conclusions The current study reveals that DES demonstrated gastroprotective effects which could be attributed to its antioxidant effect, activation of HSP-70 protein, intervention with COX-2 inflammatory pathway and potent anti H pylori effect.https://doi.org/10.1186/1472-6882-13-183Nitric OxideOmeprazoleGastric MucosaGastric UlcerGastric Lesion |
| spellingShingle | Heyam Mohamed Ali Sidahmed Ainnul Hamidah Syahadah Azizan Syam Mohan Mahmood Ameen Abdulla Siddig Ibrahim Abdelwahab Manal Mohamed Elhassan Taha A Hamid A Hadi Kamal Aziz Ketuly Najihah Mohd Hashim Mun Fai Loke Jamuna Vadivelu RETRACTED ARTICLE: Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity BMC Complementary and Alternative Medicine Nitric Oxide Omeprazole Gastric Mucosa Gastric Ulcer Gastric Lesion |
| title | RETRACTED ARTICLE: Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity |
| title_full | RETRACTED ARTICLE: Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity |
| title_fullStr | RETRACTED ARTICLE: Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity |
| title_full_unstemmed | RETRACTED ARTICLE: Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity |
| title_short | RETRACTED ARTICLE: Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity |
| title_sort | retracted article gastroprotective effect of desmosdumotin c isolated from mitrella kentii against ethanol induced gastric mucosal hemorrhage in rats possible involvement of glutathione heat shock protein 70 sulfhydryl compounds nitric oxide and anti helicobacter pylori activity |
| topic | Nitric Oxide Omeprazole Gastric Mucosa Gastric Ulcer Gastric Lesion |
| url | https://doi.org/10.1186/1472-6882-13-183 |
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