A Phytocomplex Obtained from <i>Salvia officinalis</i> by Cell Culture Technology Effectively Controls the Grapevine Downy Mildew Pathogen <i>Plasmopara viticola</i>
The negative impact of using conventional fungicides in plant disease protection has increased the interest in safer alternatives such as plant secondary metabolites, generally having a better toxicological profile. However, cultivation conditions and plant material strongly affect the quality and q...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/20/2675 |
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| author | Isabella Busato Oriana Bertaiola Silvio Tundo Chiara Guarnerio Marco Lucchetta Luca Sella Giovanna Pressi Francesco Favaron |
| author_facet | Isabella Busato Oriana Bertaiola Silvio Tundo Chiara Guarnerio Marco Lucchetta Luca Sella Giovanna Pressi Francesco Favaron |
| author_sort | Isabella Busato |
| collection | DOAJ |
| description | The negative impact of using conventional fungicides in plant disease protection has increased the interest in safer alternatives such as plant secondary metabolites, generally having a better toxicological profile. However, cultivation conditions and plant material strongly affect the quality and quantity of secondary metabolites obtained from field grown plants, limiting the standardization needed for industrial production. Plant cell culture technology can provide highly homogeneous biomasses with specific chemical characteristics. A phytocomplex with high rosmarinic acid content (10.12% <i>w</i>/<i>w</i>) was obtained from a selected cell line of <i>Salvia officinalis</i> and was tested against the grapevine downy mildew pathogen, <i>Plasmopara viticola</i>. Grapevine leaf discs were sprayed with the phytocomplex at 5 g/L and then inoculated with <i>P. viticola</i> sporangia. Sporulation level on each disc was assessed after 7 days with an image processing software. The phytocomplex reduced by 95% the sporulation level compared to the control and was also more effective than rosmarinic acid alone, used at the same concentration found in the phytocomplex. Persistence of the phytocomplex was also assessed: when applied 5 days before inoculation, it reduced by 90% the sporulation level compared to the control. These results highlight the possibility to take advantage of cell culture techniques to produce safer pesticides with high quality standards. |
| first_indexed | 2024-03-09T19:33:48Z |
| format | Article |
| id | doaj.art-976771b278684910ac2c19104052bc0b |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T19:33:48Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-976771b278684910ac2c19104052bc0b2023-11-24T02:03:16ZengMDPI AGPlants2223-77472022-10-011120267510.3390/plants11202675A Phytocomplex Obtained from <i>Salvia officinalis</i> by Cell Culture Technology Effectively Controls the Grapevine Downy Mildew Pathogen <i>Plasmopara viticola</i>Isabella Busato0Oriana Bertaiola1Silvio Tundo2Chiara Guarnerio3Marco Lucchetta4Luca Sella5Giovanna Pressi6Francesco Favaron7Department of Land, Environment, Agriculture and Forestry, University of Padova, I-35020 Legnaro, ItalyAethera Biotech s.r.l., I-36043 Camisano Vicentino, ItalyDepartment of Land, Environment, Agriculture and Forestry, University of Padova, I-35020 Legnaro, ItalyAethera Biotech s.r.l., I-36043 Camisano Vicentino, ItalyCoccitech s.r.l., I-31020 San Vendemiano, ItalyDepartment of Land, Environment, Agriculture and Forestry, University of Padova, I-35020 Legnaro, ItalyAethera Biotech s.r.l., I-36043 Camisano Vicentino, ItalyDepartment of Land, Environment, Agriculture and Forestry, University of Padova, I-35020 Legnaro, ItalyThe negative impact of using conventional fungicides in plant disease protection has increased the interest in safer alternatives such as plant secondary metabolites, generally having a better toxicological profile. However, cultivation conditions and plant material strongly affect the quality and quantity of secondary metabolites obtained from field grown plants, limiting the standardization needed for industrial production. Plant cell culture technology can provide highly homogeneous biomasses with specific chemical characteristics. A phytocomplex with high rosmarinic acid content (10.12% <i>w</i>/<i>w</i>) was obtained from a selected cell line of <i>Salvia officinalis</i> and was tested against the grapevine downy mildew pathogen, <i>Plasmopara viticola</i>. Grapevine leaf discs were sprayed with the phytocomplex at 5 g/L and then inoculated with <i>P. viticola</i> sporangia. Sporulation level on each disc was assessed after 7 days with an image processing software. The phytocomplex reduced by 95% the sporulation level compared to the control and was also more effective than rosmarinic acid alone, used at the same concentration found in the phytocomplex. Persistence of the phytocomplex was also assessed: when applied 5 days before inoculation, it reduced by 90% the sporulation level compared to the control. These results highlight the possibility to take advantage of cell culture techniques to produce safer pesticides with high quality standards.https://www.mdpi.com/2223-7747/11/20/2675<i>Plasmopara viticola</i>rosmarinic acid<i>Salvia officinalis</i>cell culture technologysecondary metabolitescrop protection |
| spellingShingle | Isabella Busato Oriana Bertaiola Silvio Tundo Chiara Guarnerio Marco Lucchetta Luca Sella Giovanna Pressi Francesco Favaron A Phytocomplex Obtained from <i>Salvia officinalis</i> by Cell Culture Technology Effectively Controls the Grapevine Downy Mildew Pathogen <i>Plasmopara viticola</i> Plants <i>Plasmopara viticola</i> rosmarinic acid <i>Salvia officinalis</i> cell culture technology secondary metabolites crop protection |
| title | A Phytocomplex Obtained from <i>Salvia officinalis</i> by Cell Culture Technology Effectively Controls the Grapevine Downy Mildew Pathogen <i>Plasmopara viticola</i> |
| title_full | A Phytocomplex Obtained from <i>Salvia officinalis</i> by Cell Culture Technology Effectively Controls the Grapevine Downy Mildew Pathogen <i>Plasmopara viticola</i> |
| title_fullStr | A Phytocomplex Obtained from <i>Salvia officinalis</i> by Cell Culture Technology Effectively Controls the Grapevine Downy Mildew Pathogen <i>Plasmopara viticola</i> |
| title_full_unstemmed | A Phytocomplex Obtained from <i>Salvia officinalis</i> by Cell Culture Technology Effectively Controls the Grapevine Downy Mildew Pathogen <i>Plasmopara viticola</i> |
| title_short | A Phytocomplex Obtained from <i>Salvia officinalis</i> by Cell Culture Technology Effectively Controls the Grapevine Downy Mildew Pathogen <i>Plasmopara viticola</i> |
| title_sort | phytocomplex obtained from i salvia officinalis i by cell culture technology effectively controls the grapevine downy mildew pathogen i plasmopara viticola i |
| topic | <i>Plasmopara viticola</i> rosmarinic acid <i>Salvia officinalis</i> cell culture technology secondary metabolites crop protection |
| url | https://www.mdpi.com/2223-7747/11/20/2675 |
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