Efficacy of T Regulatory Cells, Th17 Cells and the Associated Markers in Monitoring Tuberculosis Treatment Response

Treatment monitoring is an essential aspect for tuberculosis (TB) disease management. Sputum smear microscopy is the only available tool for monitoring, but it suffers from demerits. Therefore, we sought to evaluate markers and cellular subsets of T regulatory (Treg) cells and T helper (Th) 17 cells...

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Main Authors: Sonali Agrawal, Om Parkash, Alangudi Natarajan Palaniappan, Ashok Kumar Bhatia, Santosh Kumar, Devendra Singh Chauhan, M. Madhan Kumar
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-02-01
Series:Frontiers in Immunology
Subjects:
Online Access:http://journal.frontiersin.org/article/10.3389/fimmu.2018.00157/full
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author Sonali Agrawal
Om Parkash
Alangudi Natarajan Palaniappan
Ashok Kumar Bhatia
Santosh Kumar
Devendra Singh Chauhan
M. Madhan Kumar
author_facet Sonali Agrawal
Om Parkash
Alangudi Natarajan Palaniappan
Ashok Kumar Bhatia
Santosh Kumar
Devendra Singh Chauhan
M. Madhan Kumar
author_sort Sonali Agrawal
collection DOAJ
description Treatment monitoring is an essential aspect for tuberculosis (TB) disease management. Sputum smear microscopy is the only available tool for monitoring, but it suffers from demerits. Therefore, we sought to evaluate markers and cellular subsets of T regulatory (Treg) cells and T helper (Th) 17 cells in pulmonary TB patients (PTB) for TB treatment monitoring. Peripheral blood mononuclear cells (PBMCs) were stimulated in vitro (with purified protein derivative (PPD)) overnight which was followed by a polychromatic flow cytometry approach to study Treg and Th17 markers and cellular subsets in PTB (n = 12) undergoing antituberculous treatment (ATT). The baseline levels of these markers and cellular subsets were evaluated in normal healthy subjects (NHS). We observed a significant decrease in the expression of CD25 (p<0.01) marker and percentage of T-cell subsets like CD4+CD25+ (p<0.001) and CD4+CD25+CD39+ (p<0.05) at the end of intensive phase (IP) as well as in the continuation phase (CP) of ATT. A decrease in CD25 marker expression and percentage of CD4+CD25+ T cell subset showed a positive correlation to sputum conversion both in high and low sputum positive PTB. In eight PTB with cavitary lesions, only CD4+CD25+FoxP3 Treg subset manifested a significant decrease at the end of CP. Thus, results of this study show that CD25 marker and CD4+CD25+ T cells can serve as better markers for monitoring TB treatment efficacy. The Treg subset CD4+CD25+FoxP3 may be useful for prediction of favorable response in PTB with extensive lung lesions. However, these findings have to be evaluated in a larger patient cohort.
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spelling doaj.art-9768104e18a949448c35a1ae45afe92f2022-12-22T03:32:49ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-02-01910.3389/fimmu.2018.00157308475Efficacy of T Regulatory Cells, Th17 Cells and the Associated Markers in Monitoring Tuberculosis Treatment ResponseSonali Agrawal0Om Parkash1Alangudi Natarajan Palaniappan2Ashok Kumar Bhatia3Santosh Kumar4Devendra Singh Chauhan5M. Madhan Kumar6Department of Immunology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, IndiaDepartment of Immunology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, IndiaDepartment of Clinic, National Institute for Research in Tuberculosis, Chennai, IndiaDepartment of Biotechnology, GLA University, Mathura, IndiaDepartment of Tuberculosis and Chest Diseases, Sarojini Naidu Medical College, Agra, IndiaDepartment of Microbiology and Molecular Biology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, IndiaDepartment of Immunology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, IndiaTreatment monitoring is an essential aspect for tuberculosis (TB) disease management. Sputum smear microscopy is the only available tool for monitoring, but it suffers from demerits. Therefore, we sought to evaluate markers and cellular subsets of T regulatory (Treg) cells and T helper (Th) 17 cells in pulmonary TB patients (PTB) for TB treatment monitoring. Peripheral blood mononuclear cells (PBMCs) were stimulated in vitro (with purified protein derivative (PPD)) overnight which was followed by a polychromatic flow cytometry approach to study Treg and Th17 markers and cellular subsets in PTB (n = 12) undergoing antituberculous treatment (ATT). The baseline levels of these markers and cellular subsets were evaluated in normal healthy subjects (NHS). We observed a significant decrease in the expression of CD25 (p<0.01) marker and percentage of T-cell subsets like CD4+CD25+ (p<0.001) and CD4+CD25+CD39+ (p<0.05) at the end of intensive phase (IP) as well as in the continuation phase (CP) of ATT. A decrease in CD25 marker expression and percentage of CD4+CD25+ T cell subset showed a positive correlation to sputum conversion both in high and low sputum positive PTB. In eight PTB with cavitary lesions, only CD4+CD25+FoxP3 Treg subset manifested a significant decrease at the end of CP. Thus, results of this study show that CD25 marker and CD4+CD25+ T cells can serve as better markers for monitoring TB treatment efficacy. The Treg subset CD4+CD25+FoxP3 may be useful for prediction of favorable response in PTB with extensive lung lesions. However, these findings have to be evaluated in a larger patient cohort.http://journal.frontiersin.org/article/10.3389/fimmu.2018.00157/fulltuberculosisTregTh17treatmentmonitoring
spellingShingle Sonali Agrawal
Om Parkash
Alangudi Natarajan Palaniappan
Ashok Kumar Bhatia
Santosh Kumar
Devendra Singh Chauhan
M. Madhan Kumar
Efficacy of T Regulatory Cells, Th17 Cells and the Associated Markers in Monitoring Tuberculosis Treatment Response
Frontiers in Immunology
tuberculosis
Treg
Th17
treatment
monitoring
title Efficacy of T Regulatory Cells, Th17 Cells and the Associated Markers in Monitoring Tuberculosis Treatment Response
title_full Efficacy of T Regulatory Cells, Th17 Cells and the Associated Markers in Monitoring Tuberculosis Treatment Response
title_fullStr Efficacy of T Regulatory Cells, Th17 Cells and the Associated Markers in Monitoring Tuberculosis Treatment Response
title_full_unstemmed Efficacy of T Regulatory Cells, Th17 Cells and the Associated Markers in Monitoring Tuberculosis Treatment Response
title_short Efficacy of T Regulatory Cells, Th17 Cells and the Associated Markers in Monitoring Tuberculosis Treatment Response
title_sort efficacy of t regulatory cells th17 cells and the associated markers in monitoring tuberculosis treatment response
topic tuberculosis
Treg
Th17
treatment
monitoring
url http://journal.frontiersin.org/article/10.3389/fimmu.2018.00157/full
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