Synthesis of ginsenoside Rb1-imprinted magnetic polymer nanoparticles for the extraction and cellular delivery of therapeutic ginsenosides
Background: Panax ginseng (ginseng) is a traditional medicine that is reported to have cardioprotective effects; ginsenosides are the major bioactive compounds in the ginseng root. Methods: Magnetic molecularly imprinted polymer (MMIP) nanoparticles might be useful for both the extraction of the tar...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845322000057 |
| _version_ | 1817999709367697408 |
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| author | Kai-Hsi Liu Hung-Yin Lin James L. Thomas Yuan-Pin Shih Zhuan-Yi Yang Jen-Tsung Chen Mei-Hwa Lee |
| author_facet | Kai-Hsi Liu Hung-Yin Lin James L. Thomas Yuan-Pin Shih Zhuan-Yi Yang Jen-Tsung Chen Mei-Hwa Lee |
| author_sort | Kai-Hsi Liu |
| collection | DOAJ |
| description | Background: Panax ginseng (ginseng) is a traditional medicine that is reported to have cardioprotective effects; ginsenosides are the major bioactive compounds in the ginseng root. Methods: Magnetic molecularly imprinted polymer (MMIP) nanoparticles might be useful for both the extraction of the targeted (imprinted) molecules, and for the delivery of those molecules to cells. In this work, plant growth regulators were used to enhance the adventitious rooting of ginseng root callus; imprinted polymeric particles were synthesized for the extraction of ginsenoside Rb1 from root extracts, and then employed for subsequent particle-mediated delivery to cardiomyocytes to mitigate hypoxia/reoxygenation injury. Results: These synthesized composite nanoparticles were first characterized by their specific surface area, adsorption capacity, and magnetization, and then used for the extraction of ginsenoside Rb1 from a crude extract of ginseng roots. The ginsenoside-loaded MMIPs were then shown to have protective effects on mitochondrial membrane potential and cellular viability for H9c2 cells treated with CoCl2 to mimic hypoxia injury. The protective effect of the ginsenosides was assessed by staining with JC-1 dye to monitor the mitochondrial membrane potential. Conclusion: MMIPs can play a dual role in both the extraction and cellular delivery of therapeutic ginsenosides. |
| first_indexed | 2024-04-14T03:12:40Z |
| format | Article |
| id | doaj.art-9768341b29344dfe839bfba8b99e4c34 |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-04-14T03:12:40Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-9768341b29344dfe839bfba8b99e4c342022-12-22T02:15:33ZengElsevierJournal of Ginseng Research1226-84532022-09-01465621627Synthesis of ginsenoside Rb1-imprinted magnetic polymer nanoparticles for the extraction and cellular delivery of therapeutic ginsenosidesKai-Hsi Liu0Hung-Yin Lin1James L. Thomas2Yuan-Pin Shih3Zhuan-Yi Yang4Jen-Tsung Chen5Mei-Hwa Lee6Department of Internal Medicine, Division of Cardiology, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan; Department of Electrical Engineering, National University of Kaohsiung, Kaohsiung, TaiwanDepartment of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung, TaiwanDepartment of Physics and Astronomy, University of New Mexico, Albuquerque, NM, USADepartment of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung, TaiwanDepartment of Chemical Engineering, I-Shou University, Kaohsiung, TaiwanDepartment of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan; Corresponding author. Department of Life Sciences, National University of Kaohsiung NUK, 700, Kaohsiung University Rd, Nan-Tzu District, Kaohsiung, 811, Taiwan.Department of Materials Science and Engineering, I-Shou University, Kaohsiung, Taiwan; Corresponding author. Department of Materials Science and Engineering, I-Shou University, No.1, Sec. 1, Syuecheng Rd, Dashu District, Kaohsiung City, 84001, Taiwan.Background: Panax ginseng (ginseng) is a traditional medicine that is reported to have cardioprotective effects; ginsenosides are the major bioactive compounds in the ginseng root. Methods: Magnetic molecularly imprinted polymer (MMIP) nanoparticles might be useful for both the extraction of the targeted (imprinted) molecules, and for the delivery of those molecules to cells. In this work, plant growth regulators were used to enhance the adventitious rooting of ginseng root callus; imprinted polymeric particles were synthesized for the extraction of ginsenoside Rb1 from root extracts, and then employed for subsequent particle-mediated delivery to cardiomyocytes to mitigate hypoxia/reoxygenation injury. Results: These synthesized composite nanoparticles were first characterized by their specific surface area, adsorption capacity, and magnetization, and then used for the extraction of ginsenoside Rb1 from a crude extract of ginseng roots. The ginsenoside-loaded MMIPs were then shown to have protective effects on mitochondrial membrane potential and cellular viability for H9c2 cells treated with CoCl2 to mimic hypoxia injury. The protective effect of the ginsenosides was assessed by staining with JC-1 dye to monitor the mitochondrial membrane potential. Conclusion: MMIPs can play a dual role in both the extraction and cellular delivery of therapeutic ginsenosides.http://www.sciencedirect.com/science/article/pii/S1226845322000057Ginseng rootGinsenoside Rb1Molecular imprintingExtractionMyocyte |
| spellingShingle | Kai-Hsi Liu Hung-Yin Lin James L. Thomas Yuan-Pin Shih Zhuan-Yi Yang Jen-Tsung Chen Mei-Hwa Lee Synthesis of ginsenoside Rb1-imprinted magnetic polymer nanoparticles for the extraction and cellular delivery of therapeutic ginsenosides Journal of Ginseng Research Ginseng root Ginsenoside Rb1 Molecular imprinting Extraction Myocyte |
| title | Synthesis of ginsenoside Rb1-imprinted magnetic polymer nanoparticles for the extraction and cellular delivery of therapeutic ginsenosides |
| title_full | Synthesis of ginsenoside Rb1-imprinted magnetic polymer nanoparticles for the extraction and cellular delivery of therapeutic ginsenosides |
| title_fullStr | Synthesis of ginsenoside Rb1-imprinted magnetic polymer nanoparticles for the extraction and cellular delivery of therapeutic ginsenosides |
| title_full_unstemmed | Synthesis of ginsenoside Rb1-imprinted magnetic polymer nanoparticles for the extraction and cellular delivery of therapeutic ginsenosides |
| title_short | Synthesis of ginsenoside Rb1-imprinted magnetic polymer nanoparticles for the extraction and cellular delivery of therapeutic ginsenosides |
| title_sort | synthesis of ginsenoside rb1 imprinted magnetic polymer nanoparticles for the extraction and cellular delivery of therapeutic ginsenosides |
| topic | Ginseng root Ginsenoside Rb1 Molecular imprinting Extraction Myocyte |
| url | http://www.sciencedirect.com/science/article/pii/S1226845322000057 |
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