Inhibition of Foodborne Pathogenic Bacteria by Excreted Metabolites of <i>Serratia marcescens</i> Strains Isolated from a Dairy-Producing Environment
<i>Serratia marcescens</i> strains from a dairy-producing environment were tested for their inhibitory effect on <i>Listeria monocytogenes</i>, <i>Salmonella</i> Hartford, <i>Yersinia enterocolitica</i> and <i>Escherichia coli</i>. Inhibiti...
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MDPI AG
2023-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/2/403 |
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| author | Bernadett Baráti-Deák Giseli Cristina Da Costa Arruda Judit Perjéssy Adél Klupács Zsolt Zalán Csilla Mohácsi-Farkas Ágnes Belák |
| author_facet | Bernadett Baráti-Deák Giseli Cristina Da Costa Arruda Judit Perjéssy Adél Klupács Zsolt Zalán Csilla Mohácsi-Farkas Ágnes Belák |
| author_sort | Bernadett Baráti-Deák |
| collection | DOAJ |
| description | <i>Serratia marcescens</i> strains from a dairy-producing environment were tested for their inhibitory effect on <i>Listeria monocytogenes</i>, <i>Salmonella</i> Hartford, <i>Yersinia enterocolitica</i> and <i>Escherichia coli</i>. Inhibition of foodborne pathogens was observed in the case of a non-pigmented <i>Serratia</i> strain, while the pigment-producing isolate was able to inhibit only <i>Y. enterocolitica</i>. The co-culturing study in tryptone soya broth (TSB) and milk showed that the growth of <i>Salmonella</i> was inhibited in the first 24 h, but later the pathogen could grow in the presence of the <i>Serratia</i> strain even if its cell concentration was 1000 times higher than that of <i>Salmonella</i>. However, we found that (1) concentrated cell-free supernatants had stronger inhibitory activity, which confirms the extracellular nature of the antagonistic compound(s). We proved that (2) protease and chitinase enzymes can take part in this mechanism, but they are not the main inhibitory compounds. The presence of prodigiosin was observed only in the case of the pigmented strain; thus, (3) we hypothesized that prodigiosin does not take part in the inhibition of the pathogens. However, (4) the combined effect of different extracellular metabolites might be attributed to the inhibitory property. Application of concentrated <i>S. marcescens</i> cell-free supernatant can be an effective antibacterial strategy in the food industry, mainly in the form of a bio-disinfectant on surfaces of food-processing areas. |
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| language | English |
| last_indexed | 2024-03-11T08:23:17Z |
| publishDate | 2023-02-01 |
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| series | Microorganisms |
| spelling | doaj.art-976b4daeddc2497eaa0b31a8bc54f3422023-11-16T22:15:22ZengMDPI AGMicroorganisms2076-26072023-02-0111240310.3390/microorganisms11020403Inhibition of Foodborne Pathogenic Bacteria by Excreted Metabolites of <i>Serratia marcescens</i> Strains Isolated from a Dairy-Producing EnvironmentBernadett Baráti-Deák0Giseli Cristina Da Costa Arruda1Judit Perjéssy2Adél Klupács3Zsolt Zalán4Csilla Mohácsi-Farkas5Ágnes Belák6Department of Food Microbiology, Hygiene and Safety, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Somlói út 14-16, H-1118 Budapest, HungaryDepartment of Food Microbiology, Hygiene and Safety, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Somlói út 14-16, H-1118 Budapest, HungaryDepartment of Nutrition, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Somlói út, 14-16, H-1118 Budapest, HungaryDepartment of Nutrition, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Somlói út, 14-16, H-1118 Budapest, HungaryDepartment of Bioengineering and Fermentation Technology, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Ménesi út 45, H-1118 Budapest, HungaryDepartment of Food Microbiology, Hygiene and Safety, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Somlói út 14-16, H-1118 Budapest, HungaryDepartment of Food Microbiology, Hygiene and Safety, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Somlói út 14-16, H-1118 Budapest, Hungary<i>Serratia marcescens</i> strains from a dairy-producing environment were tested for their inhibitory effect on <i>Listeria monocytogenes</i>, <i>Salmonella</i> Hartford, <i>Yersinia enterocolitica</i> and <i>Escherichia coli</i>. Inhibition of foodborne pathogens was observed in the case of a non-pigmented <i>Serratia</i> strain, while the pigment-producing isolate was able to inhibit only <i>Y. enterocolitica</i>. The co-culturing study in tryptone soya broth (TSB) and milk showed that the growth of <i>Salmonella</i> was inhibited in the first 24 h, but later the pathogen could grow in the presence of the <i>Serratia</i> strain even if its cell concentration was 1000 times higher than that of <i>Salmonella</i>. However, we found that (1) concentrated cell-free supernatants had stronger inhibitory activity, which confirms the extracellular nature of the antagonistic compound(s). We proved that (2) protease and chitinase enzymes can take part in this mechanism, but they are not the main inhibitory compounds. The presence of prodigiosin was observed only in the case of the pigmented strain; thus, (3) we hypothesized that prodigiosin does not take part in the inhibition of the pathogens. However, (4) the combined effect of different extracellular metabolites might be attributed to the inhibitory property. Application of concentrated <i>S. marcescens</i> cell-free supernatant can be an effective antibacterial strategy in the food industry, mainly in the form of a bio-disinfectant on surfaces of food-processing areas.https://www.mdpi.com/2076-2607/11/2/403food safetynatural antimicrobialsantibacterial activityhydrolytic enzymesprodigiosinextracellular metabolites |
| spellingShingle | Bernadett Baráti-Deák Giseli Cristina Da Costa Arruda Judit Perjéssy Adél Klupács Zsolt Zalán Csilla Mohácsi-Farkas Ágnes Belák Inhibition of Foodborne Pathogenic Bacteria by Excreted Metabolites of <i>Serratia marcescens</i> Strains Isolated from a Dairy-Producing Environment Microorganisms food safety natural antimicrobials antibacterial activity hydrolytic enzymes prodigiosin extracellular metabolites |
| title | Inhibition of Foodborne Pathogenic Bacteria by Excreted Metabolites of <i>Serratia marcescens</i> Strains Isolated from a Dairy-Producing Environment |
| title_full | Inhibition of Foodborne Pathogenic Bacteria by Excreted Metabolites of <i>Serratia marcescens</i> Strains Isolated from a Dairy-Producing Environment |
| title_fullStr | Inhibition of Foodborne Pathogenic Bacteria by Excreted Metabolites of <i>Serratia marcescens</i> Strains Isolated from a Dairy-Producing Environment |
| title_full_unstemmed | Inhibition of Foodborne Pathogenic Bacteria by Excreted Metabolites of <i>Serratia marcescens</i> Strains Isolated from a Dairy-Producing Environment |
| title_short | Inhibition of Foodborne Pathogenic Bacteria by Excreted Metabolites of <i>Serratia marcescens</i> Strains Isolated from a Dairy-Producing Environment |
| title_sort | inhibition of foodborne pathogenic bacteria by excreted metabolites of i serratia marcescens i strains isolated from a dairy producing environment |
| topic | food safety natural antimicrobials antibacterial activity hydrolytic enzymes prodigiosin extracellular metabolites |
| url | https://www.mdpi.com/2076-2607/11/2/403 |
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