Characterisation of the Gut Bacteria of Cultured and Wild Spiny Lobster <i>Panulirus ornatus</i>
The commercial onshore aquaculture of the spiny lobster <i>Panulirus ornatus</i>, while in its infancy, has progressed rapidly from the enabling research that continues at the University of Tasmania. The development of lobster feeds, both fresh and manufactured, has been critical to the...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Applied Microbiology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-8007/3/1/16 |
| _version_ | 1797613798237929472 |
|---|---|
| author | Mei C. Ooi Andrew J. Trotter Gregory G. Smith Andrew R. Bridle |
| author_facet | Mei C. Ooi Andrew J. Trotter Gregory G. Smith Andrew R. Bridle |
| author_sort | Mei C. Ooi |
| collection | DOAJ |
| description | The commercial onshore aquaculture of the spiny lobster <i>Panulirus ornatus</i>, while in its infancy, has progressed rapidly from the enabling research that continues at the University of Tasmania. The development of lobster feeds, both fresh and manufactured, has been critical to the success of this emerging aquaculture sector. Fresh feeds derived from mussel represent the gold standard in terms of the growth performance of juvenile lobsters. Nonetheless, concerns regarding availability, sustainability, and potential biosecurity issues of fresh feeds highlight the importance of developing manufactured feeds for lobster aquaculture. Wild lobsters are assumed to have a balanced natural diet that allows for standard growth and development, and as such natural diets are often used as a reference for feed development. Similarly, the gut microbiota associated with a natural diet is assumed to reflect a healthy microbial assemblage. The aim of this study was to compare the microbiota of the hindgut and hepatopancreas of cultured <i>P. ornatus</i> fed with a commercial prawn pellet or mussel to that of wild spiny lobster juveniles. Gut samples were analysed using Oxford Nanopore 16S rRNA gene sequencing. Based on principal coordinate analysis, the gut bacteria of cultured lobsters were different from the wild juveniles. The core microbiota of the hindgut and hepatopancreas libraries were phyla Proteobacteria (Gamma, Alpha) and Bacteroidetes. <i>Vibrio</i> was the most dominant genus in both organs. The differences in bacterial relative abundance were mainly between cultured (pellet-, mussel-fed) and wild lobsters. In conclusion, bacteria in the cultured lobsters had significantly different profiles to that of the wild juveniles, indicating that current onshore aquaculture practices alter the gut microbiota. A number of different feeding and culture practices may be required if the aim of closed culture practices is to attain a gut microbiota in cultured animals that is representative of that found in wild spiny lobsters. |
| first_indexed | 2024-03-11T06:59:45Z |
| format | Article |
| id | doaj.art-976c461fc44d484e870ca7f8ff89e020 |
| institution | Directory Open Access Journal |
| issn | 2673-8007 |
| language | English |
| last_indexed | 2024-03-11T06:59:45Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Microbiology |
| spelling | doaj.art-976c461fc44d484e870ca7f8ff89e0202023-11-17T09:20:39ZengMDPI AGApplied Microbiology2673-80072023-02-013124125310.3390/applmicrobiol3010016Characterisation of the Gut Bacteria of Cultured and Wild Spiny Lobster <i>Panulirus ornatus</i>Mei C. Ooi0Andrew J. Trotter1Gregory G. Smith2Andrew R. Bridle3Institute for Marine and Antarctic Studies, University of Tasmania, Launceston, TAS 7250, AustraliaInstitute for Marine and Antarctic Studies, University of Tasmania, Launceston, TAS 7250, AustraliaInstitute for Marine and Antarctic Studies, University of Tasmania, Launceston, TAS 7250, AustraliaInstitute for Marine and Antarctic Studies, University of Tasmania, Launceston, TAS 7250, AustraliaThe commercial onshore aquaculture of the spiny lobster <i>Panulirus ornatus</i>, while in its infancy, has progressed rapidly from the enabling research that continues at the University of Tasmania. The development of lobster feeds, both fresh and manufactured, has been critical to the success of this emerging aquaculture sector. Fresh feeds derived from mussel represent the gold standard in terms of the growth performance of juvenile lobsters. Nonetheless, concerns regarding availability, sustainability, and potential biosecurity issues of fresh feeds highlight the importance of developing manufactured feeds for lobster aquaculture. Wild lobsters are assumed to have a balanced natural diet that allows for standard growth and development, and as such natural diets are often used as a reference for feed development. Similarly, the gut microbiota associated with a natural diet is assumed to reflect a healthy microbial assemblage. The aim of this study was to compare the microbiota of the hindgut and hepatopancreas of cultured <i>P. ornatus</i> fed with a commercial prawn pellet or mussel to that of wild spiny lobster juveniles. Gut samples were analysed using Oxford Nanopore 16S rRNA gene sequencing. Based on principal coordinate analysis, the gut bacteria of cultured lobsters were different from the wild juveniles. The core microbiota of the hindgut and hepatopancreas libraries were phyla Proteobacteria (Gamma, Alpha) and Bacteroidetes. <i>Vibrio</i> was the most dominant genus in both organs. The differences in bacterial relative abundance were mainly between cultured (pellet-, mussel-fed) and wild lobsters. In conclusion, bacteria in the cultured lobsters had significantly different profiles to that of the wild juveniles, indicating that current onshore aquaculture practices alter the gut microbiota. A number of different feeding and culture practices may be required if the aim of closed culture practices is to attain a gut microbiota in cultured animals that is representative of that found in wild spiny lobsters.https://www.mdpi.com/2673-8007/3/1/16gut bacteriawild spiny lobstercultured spiny lobstermusselpellet<i>Vibrio</i> |
| spellingShingle | Mei C. Ooi Andrew J. Trotter Gregory G. Smith Andrew R. Bridle Characterisation of the Gut Bacteria of Cultured and Wild Spiny Lobster <i>Panulirus ornatus</i> Applied Microbiology gut bacteria wild spiny lobster cultured spiny lobster mussel pellet <i>Vibrio</i> |
| title | Characterisation of the Gut Bacteria of Cultured and Wild Spiny Lobster <i>Panulirus ornatus</i> |
| title_full | Characterisation of the Gut Bacteria of Cultured and Wild Spiny Lobster <i>Panulirus ornatus</i> |
| title_fullStr | Characterisation of the Gut Bacteria of Cultured and Wild Spiny Lobster <i>Panulirus ornatus</i> |
| title_full_unstemmed | Characterisation of the Gut Bacteria of Cultured and Wild Spiny Lobster <i>Panulirus ornatus</i> |
| title_short | Characterisation of the Gut Bacteria of Cultured and Wild Spiny Lobster <i>Panulirus ornatus</i> |
| title_sort | characterisation of the gut bacteria of cultured and wild spiny lobster i panulirus ornatus i |
| topic | gut bacteria wild spiny lobster cultured spiny lobster mussel pellet <i>Vibrio</i> |
| url | https://www.mdpi.com/2673-8007/3/1/16 |
| work_keys_str_mv | AT meicooi characterisationofthegutbacteriaofculturedandwildspinylobsteripanulirusornatusi AT andrewjtrotter characterisationofthegutbacteriaofculturedandwildspinylobsteripanulirusornatusi AT gregorygsmith characterisationofthegutbacteriaofculturedandwildspinylobsteripanulirusornatusi AT andrewrbridle characterisationofthegutbacteriaofculturedandwildspinylobsteripanulirusornatusi |