Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites
For more than 50 years, the CIAM-AGACAL (Agricultural Research Centre of Mabegondo, Xunta de Galicia, A Coruña, Spain) has been carrying out the important task of conserving the phytogenetic resources of ecotypes and natural populations of grassland species from northwestern Spain. The CIAM-AGACAL’s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/11/2275 |
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| author | Cristina Isabel Fernández-Otero Ana María Ramos-Cabrer Julio Enrique López-Díaz Santiago Pereira-Lorenzo |
| author_facet | Cristina Isabel Fernández-Otero Ana María Ramos-Cabrer Julio Enrique López-Díaz Santiago Pereira-Lorenzo |
| author_sort | Cristina Isabel Fernández-Otero |
| collection | DOAJ |
| description | For more than 50 years, the CIAM-AGACAL (Agricultural Research Centre of Mabegondo, Xunta de Galicia, A Coruña, Spain) has been carrying out the important task of conserving the phytogenetic resources of ecotypes and natural populations of grassland species from northwestern Spain. The CIAM-AGACAL’s germplasm bank has 57 populations of red clover (<i>Trifolium pratense</i> Lam.), one of the most cultivated forage legumes in the world. The goal of the present study was to evaluate the diversity among cultivars and natural clover populations at morphological and molecular level. Twelve polymorphic SSR loci revealed 241 microsatellite alleles with an average of 20.08 alleles per locus. Two main groups were detected by the Structure software, one of them including local populations and the second clustering cultivars and related populations. Intra-specific variability was found among cultivars and natural populations. A moderate genetic differentiation of Spanish red clover cultivars was observed (Fst = 0.08) between the two main clusters. Finally, a certain relationship between phenotypic and genotypic variation was detected. |
| first_indexed | 2024-03-10T05:47:16Z |
| format | Article |
| id | doaj.art-976d6be3c24241a587ded17d12e45000 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T05:47:16Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-976d6be3c24241a587ded17d12e450002023-11-22T22:03:09ZengMDPI AGAgronomy2073-43952021-11-011111227510.3390/agronomy11112275Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and MicrosatellitesCristina Isabel Fernández-Otero0Ana María Ramos-Cabrer1Julio Enrique López-Díaz2Santiago Pereira-Lorenzo3Department of Crop Production, Centro de Investigaciones Agrarias de Mabegondo, AGACAL, 15080 La Coruña, SpainDepartment of Crop Production and Engineering Projects, Universidade de Santiago de Compostela, Campus de Lugo, 27002 Lugo, SpainDepartment of Crop Production, Centro de Investigaciones Agrarias de Mabegondo, AGACAL, 15080 La Coruña, SpainDepartment of Crop Production and Engineering Projects, Universidade de Santiago de Compostela, Campus de Lugo, 27002 Lugo, SpainFor more than 50 years, the CIAM-AGACAL (Agricultural Research Centre of Mabegondo, Xunta de Galicia, A Coruña, Spain) has been carrying out the important task of conserving the phytogenetic resources of ecotypes and natural populations of grassland species from northwestern Spain. The CIAM-AGACAL’s germplasm bank has 57 populations of red clover (<i>Trifolium pratense</i> Lam.), one of the most cultivated forage legumes in the world. The goal of the present study was to evaluate the diversity among cultivars and natural clover populations at morphological and molecular level. Twelve polymorphic SSR loci revealed 241 microsatellite alleles with an average of 20.08 alleles per locus. Two main groups were detected by the Structure software, one of them including local populations and the second clustering cultivars and related populations. Intra-specific variability was found among cultivars and natural populations. A moderate genetic differentiation of Spanish red clover cultivars was observed (Fst = 0.08) between the two main clusters. Finally, a certain relationship between phenotypic and genotypic variation was detected.https://www.mdpi.com/2073-4395/11/11/2275simple sequence repeats (SSRs)variabilitymorphologylocal cultivarsgermplasm bank |
| spellingShingle | Cristina Isabel Fernández-Otero Ana María Ramos-Cabrer Julio Enrique López-Díaz Santiago Pereira-Lorenzo Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites Agronomy simple sequence repeats (SSRs) variability morphology local cultivars germplasm bank |
| title | Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites |
| title_full | Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites |
| title_fullStr | Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites |
| title_full_unstemmed | Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites |
| title_short | Evaluating the Diversity of Ecotypes of Red Clover (<i>Trifolium pratense</i> L.) from Northwestern Spain by Phenotypic Traits and Microsatellites |
| title_sort | evaluating the diversity of ecotypes of red clover i trifolium pratense i l from northwestern spain by phenotypic traits and microsatellites |
| topic | simple sequence repeats (SSRs) variability morphology local cultivars germplasm bank |
| url | https://www.mdpi.com/2073-4395/11/11/2275 |
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