lncRNA GAPLINC regulates vascular endothelial cell apoptosis in atherosclerosis
Introduction In this study, we investigated the role of the long non-coding RNA GAPLINC in atherosclerosis under oxidized low-density lipoprotein (ox-LDL) treatment. Material and methods We utilized ox-LDL exposed human aortic endothelial cells as an in-vitro model. The expression level of GAPLINC...
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| Format: | Article |
| Language: | English |
| Published: |
Termedia Publishing House
2023-07-01
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| Series: | Archives of Medical Science |
| Subjects: | |
| Online Access: | https://www.archivesofmedicalscience.com/lncRNA-GAPLINC-regulates-vascular-endothelial-cell-apoptosis-in-atherosclerosis,169383,0,2.html |
| _version_ | 1827287693979025408 |
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| author | Jun Pan Bing Wang Xibin Pu Chenyang Qiu Donglin Li Ziheng Wu Hongkun Zhang Yangyan He |
| author_facet | Jun Pan Bing Wang Xibin Pu Chenyang Qiu Donglin Li Ziheng Wu Hongkun Zhang Yangyan He |
| author_sort | Jun Pan |
| collection | DOAJ |
| description | Introduction
In this study, we investigated the role of the long non-coding RNA GAPLINC in atherosclerosis under oxidized low-density lipoprotein (ox-LDL) treatment.
Material and methods
We utilized ox-LDL exposed human aortic endothelial cells as an in-vitro model. The expression level of GAPLINC was quantified by qPCR in different times and concentrations of ox-LDL treatment conditions. Cell apoptosis rate and cell cycle profiles were assessed by flow cytometry and TUNEL assay. The target association was confirmed using a luciferase reporter assay and Western blot.
Results
We found that GAPLINC expression was induced by ox-LDL treatment, but cell proliferation ability was significantly inhibited. We further confirmed that overexpressing of lncRNA GAPLINC in ox-LDL-exposed HAECs decreased cell proliferation by increasing cell apoptosis and arresting cell cycle in G2/M and S phase. Importantly, the detailed mechanistic analysis elucidated that LncRNA GAPLINC acts as a decoy to sequester miR-183-5p to prevent it from binding to target PDCD4. MiR-183-5p targets GAPLINC, and PDCD4 is a downstream target of miR-183-5p, and the cellular effects of this direct interaction were confirmed by a rescue assay experiment.
Conclusions
The present study demonstrates that upregulation of lncRNA GAPLINC represses the binding of miR-183-5p to the PDCD4 promoter region and then promotes PDCD4 expression, thereby inducing cell apoptosis and suppressing endothelial cell proliferation. |
| first_indexed | 2024-04-24T11:16:15Z |
| format | Article |
| id | doaj.art-976d89ada7ff4eb6b7101eaa77fc01d6 |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-04-24T11:16:15Z |
| publishDate | 2023-07-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-976d89ada7ff4eb6b7101eaa77fc01d62024-04-11T08:11:16ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512023-07-0120121623210.5114/aoms/169383169383lncRNA GAPLINC regulates vascular endothelial cell apoptosis in atherosclerosisJun Pan0Bing Wang1Xibin Pu2Chenyang Qiu3Donglin Li4Ziheng Wu5Hongkun Zhang6Yangyan He7Department of Vascular Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, ChinaDepartment of Vascular Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, ChinaDepartment of Vascular Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, ChinaDepartment of Vascular Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, ChinaDepartment of Vascular Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, ChinaDepartment of Vascular Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, ChinaDepartment of Vascular Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, ChinaDepartment of Vascular Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, ChinaIntroduction In this study, we investigated the role of the long non-coding RNA GAPLINC in atherosclerosis under oxidized low-density lipoprotein (ox-LDL) treatment. Material and methods We utilized ox-LDL exposed human aortic endothelial cells as an in-vitro model. The expression level of GAPLINC was quantified by qPCR in different times and concentrations of ox-LDL treatment conditions. Cell apoptosis rate and cell cycle profiles were assessed by flow cytometry and TUNEL assay. The target association was confirmed using a luciferase reporter assay and Western blot. Results We found that GAPLINC expression was induced by ox-LDL treatment, but cell proliferation ability was significantly inhibited. We further confirmed that overexpressing of lncRNA GAPLINC in ox-LDL-exposed HAECs decreased cell proliferation by increasing cell apoptosis and arresting cell cycle in G2/M and S phase. Importantly, the detailed mechanistic analysis elucidated that LncRNA GAPLINC acts as a decoy to sequester miR-183-5p to prevent it from binding to target PDCD4. MiR-183-5p targets GAPLINC, and PDCD4 is a downstream target of miR-183-5p, and the cellular effects of this direct interaction were confirmed by a rescue assay experiment. Conclusions The present study demonstrates that upregulation of lncRNA GAPLINC represses the binding of miR-183-5p to the PDCD4 promoter region and then promotes PDCD4 expression, thereby inducing cell apoptosis and suppressing endothelial cell proliferation.https://www.archivesofmedicalscience.com/lncRNA-GAPLINC-regulates-vascular-endothelial-cell-apoptosis-in-atherosclerosis,169383,0,2.htmllong non-coding rnagaplincox-ldlatherosclerosiscell apoptosis |
| spellingShingle | Jun Pan Bing Wang Xibin Pu Chenyang Qiu Donglin Li Ziheng Wu Hongkun Zhang Yangyan He lncRNA GAPLINC regulates vascular endothelial cell apoptosis in atherosclerosis Archives of Medical Science long non-coding rna gaplinc ox-ldl atherosclerosis cell apoptosis |
| title | lncRNA GAPLINC regulates vascular endothelial cell apoptosis in atherosclerosis |
| title_full | lncRNA GAPLINC regulates vascular endothelial cell apoptosis in atherosclerosis |
| title_fullStr | lncRNA GAPLINC regulates vascular endothelial cell apoptosis in atherosclerosis |
| title_full_unstemmed | lncRNA GAPLINC regulates vascular endothelial cell apoptosis in atherosclerosis |
| title_short | lncRNA GAPLINC regulates vascular endothelial cell apoptosis in atherosclerosis |
| title_sort | lncrna gaplinc regulates vascular endothelial cell apoptosis in atherosclerosis |
| topic | long non-coding rna gaplinc ox-ldl atherosclerosis cell apoptosis |
| url | https://www.archivesofmedicalscience.com/lncRNA-GAPLINC-regulates-vascular-endothelial-cell-apoptosis-in-atherosclerosis,169383,0,2.html |
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