The presence of membrane bound CD99 ligands on leukocyte surface
Abstract Objective CD99, a leukocyte surface molecule, reportedly plays an important role in several cellular processes. However, the role of CD99 in T cell regulation remains unclear, as the CD99 ligand associated with T-cell regulation has not yet been identified. Our previous study showed that re...
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| Format: | Article |
| Language: | English |
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BMC
2020-10-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-020-05347-0 |
| _version_ | 1818234381636993024 |
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| author | Nuchjira Takheaw Supansa Pata Witida Laopajon Sittiruk Roytrakul Watchara Kasinrerk |
| author_facet | Nuchjira Takheaw Supansa Pata Witida Laopajon Sittiruk Roytrakul Watchara Kasinrerk |
| author_sort | Nuchjira Takheaw |
| collection | DOAJ |
| description | Abstract Objective CD99, a leukocyte surface molecule, reportedly plays an important role in several cellular processes. However, the role of CD99 in T cell regulation remains unclear, as the CD99 ligand associated with T-cell regulation has not yet been identified. Our previous study showed that recombinant CD99 bound to CD99 ligands was expressed on monocytes, NK cells and dendritic cells. This interaction regulates the expression of IL-6 and TNF-α in CD3 + T cells following T cell activation. In the present study, we confirmed the presence of CD99 ligands in immune cells. Results A recombinant CD99-human IgG fusion protein, CD99HIgG, was produced and used to search for CD99 ligand expression in various hematopoietic cell lines. Among several cell lines, THP-1 monocytic cell line showed strong positive reaction for CD99HIgG, and CD99 and CD99 ligand complexes were pulled-down using a DTSSP cross-linker. The study demonstrated the presence of the membrane bound CD99 ligand, and CD99 ligand candidates were identified via LC–MS/MS. These results may be useful to further identify the CD99 ligands, and to fully comprehend the role of CD99 in immunoregulation. |
| first_indexed | 2024-12-12T11:37:10Z |
| format | Article |
| id | doaj.art-976e8738ff1e412d8e2f398c50479538 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-12T11:37:10Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-976e8738ff1e412d8e2f398c504795382022-12-22T00:25:38ZengBMCBMC Research Notes1756-05002020-10-011311610.1186/s13104-020-05347-0The presence of membrane bound CD99 ligands on leukocyte surfaceNuchjira Takheaw0Supansa Pata1Witida Laopajon2Sittiruk Roytrakul3Watchara Kasinrerk4Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityDivision of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityDivision of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityFunctional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development AgencyDivision of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityAbstract Objective CD99, a leukocyte surface molecule, reportedly plays an important role in several cellular processes. However, the role of CD99 in T cell regulation remains unclear, as the CD99 ligand associated with T-cell regulation has not yet been identified. Our previous study showed that recombinant CD99 bound to CD99 ligands was expressed on monocytes, NK cells and dendritic cells. This interaction regulates the expression of IL-6 and TNF-α in CD3 + T cells following T cell activation. In the present study, we confirmed the presence of CD99 ligands in immune cells. Results A recombinant CD99-human IgG fusion protein, CD99HIgG, was produced and used to search for CD99 ligand expression in various hematopoietic cell lines. Among several cell lines, THP-1 monocytic cell line showed strong positive reaction for CD99HIgG, and CD99 and CD99 ligand complexes were pulled-down using a DTSSP cross-linker. The study demonstrated the presence of the membrane bound CD99 ligand, and CD99 ligand candidates were identified via LC–MS/MS. These results may be useful to further identify the CD99 ligands, and to fully comprehend the role of CD99 in immunoregulation.http://link.springer.com/article/10.1186/s13104-020-05347-0CD99 ligandCD99Recombinant CD99LC–MS/MSLeukocyte |
| spellingShingle | Nuchjira Takheaw Supansa Pata Witida Laopajon Sittiruk Roytrakul Watchara Kasinrerk The presence of membrane bound CD99 ligands on leukocyte surface BMC Research Notes CD99 ligand CD99 Recombinant CD99 LC–MS/MS Leukocyte |
| title | The presence of membrane bound CD99 ligands on leukocyte surface |
| title_full | The presence of membrane bound CD99 ligands on leukocyte surface |
| title_fullStr | The presence of membrane bound CD99 ligands on leukocyte surface |
| title_full_unstemmed | The presence of membrane bound CD99 ligands on leukocyte surface |
| title_short | The presence of membrane bound CD99 ligands on leukocyte surface |
| title_sort | presence of membrane bound cd99 ligands on leukocyte surface |
| topic | CD99 ligand CD99 Recombinant CD99 LC–MS/MS Leukocyte |
| url | http://link.springer.com/article/10.1186/s13104-020-05347-0 |
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