Comparison of repair index in cigarette and waterpipe smokers: A bio-monitoring assessment using human exfoliated buccal mucosa cells
Background: Repair index (RI) using DNA changes reveals the activity of carcinogenesis. Cigarette and waterpipe smoking are important contributors to oral cavity malignancy. The RI in cigarette and waterpipe smokers has not been compared so far. The aim of this study was to compare the RI in cigaret...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | International Journal of Preventive Medicine |
| Subjects: | |
| Online Access: | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2022;volume=13;issue=1;spage=27;epage=27;aulast=Jalili |
| _version_ | 1818489422606237696 |
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| author | Sana Jalili Noushin Jalayer Naderi |
| author_facet | Sana Jalili Noushin Jalayer Naderi |
| author_sort | Sana Jalili |
| collection | DOAJ |
| description | Background: Repair index (RI) using DNA changes reveals the activity of carcinogenesis. Cigarette and waterpipe smoking are important contributors to oral cavity malignancy. The RI in cigarette and waterpipe smokers has not been compared so far. The aim of this study was to compare the RI in cigarette and waterpipe smokers using the human exfoliated buccal mucosa cells. Methods: The exfoliated buccal mucosa cells of 60 cigarette and waterpipe smokers and 20 nonsmokers were evaluated in a case-control study. The number of micronuclei (MN), broken egg (BE), karyorrhexis (KR), and karyolysis (KL) were counted in 1000 cells from randomly selected fields. The RI = (KL + KR)/(MN + BE) was calculated and compared between subjects and controls. Data were analyzed by one-way analysis of variance (ANOVA), Tukey's Honest Significant Difference (HSD), and Spearman's correlation coefficient test at P < 0.05 probability level. Results: The difference of MN (P < 0.0001), BE (P < 0.0001), KR (P < 0.0001), and KL (P < 0.0001) count was significant between cigarette smokers, waterpipe smokers, and nonsmokers. The RI was significantly different between groups (P = 0.007). The RI was significantly higher in cigarette smokers compared to waterpipe smokers (P = 0.04) and nonsmokers (P = 0.009). Conclusions: The RI was significantly higher in cigarette smokers compared to waterpipe smokers. The finding suggests that due to higher interrupted cellular hemostasis, the risk of carcinoma in waterpipe smokers can be greater than that in cigarette smokers. |
| first_indexed | 2024-12-10T17:03:45Z |
| format | Article |
| id | doaj.art-9773797a0f554120ba7c4f724389f7a9 |
| institution | Directory Open Access Journal |
| issn | 2008-7802 2008-8213 |
| language | English |
| last_indexed | 2024-12-10T17:03:45Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Preventive Medicine |
| spelling | doaj.art-9773797a0f554120ba7c4f724389f7a92022-12-22T01:40:30ZengWolters Kluwer Medknow PublicationsInternational Journal of Preventive Medicine2008-78022008-82132022-01-01131272710.4103/ijpvm.IJPVM_10_20Comparison of repair index in cigarette and waterpipe smokers: A bio-monitoring assessment using human exfoliated buccal mucosa cellsSana JaliliNoushin Jalayer NaderiBackground: Repair index (RI) using DNA changes reveals the activity of carcinogenesis. Cigarette and waterpipe smoking are important contributors to oral cavity malignancy. The RI in cigarette and waterpipe smokers has not been compared so far. The aim of this study was to compare the RI in cigarette and waterpipe smokers using the human exfoliated buccal mucosa cells. Methods: The exfoliated buccal mucosa cells of 60 cigarette and waterpipe smokers and 20 nonsmokers were evaluated in a case-control study. The number of micronuclei (MN), broken egg (BE), karyorrhexis (KR), and karyolysis (KL) were counted in 1000 cells from randomly selected fields. The RI = (KL + KR)/(MN + BE) was calculated and compared between subjects and controls. Data were analyzed by one-way analysis of variance (ANOVA), Tukey's Honest Significant Difference (HSD), and Spearman's correlation coefficient test at P < 0.05 probability level. Results: The difference of MN (P < 0.0001), BE (P < 0.0001), KR (P < 0.0001), and KL (P < 0.0001) count was significant between cigarette smokers, waterpipe smokers, and nonsmokers. The RI was significantly different between groups (P = 0.007). The RI was significantly higher in cigarette smokers compared to waterpipe smokers (P = 0.04) and nonsmokers (P = 0.009). Conclusions: The RI was significantly higher in cigarette smokers compared to waterpipe smokers. The finding suggests that due to higher interrupted cellular hemostasis, the risk of carcinoma in waterpipe smokers can be greater than that in cigarette smokers.http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2022;volume=13;issue=1;spage=27;epage=27;aulast=Jalilimicronucleus assaysmouth mucosasmoking |
| spellingShingle | Sana Jalili Noushin Jalayer Naderi Comparison of repair index in cigarette and waterpipe smokers: A bio-monitoring assessment using human exfoliated buccal mucosa cells International Journal of Preventive Medicine micronucleus assays mouth mucosa smoking |
| title | Comparison of repair index in cigarette and waterpipe smokers: A bio-monitoring assessment using human exfoliated buccal mucosa cells |
| title_full | Comparison of repair index in cigarette and waterpipe smokers: A bio-monitoring assessment using human exfoliated buccal mucosa cells |
| title_fullStr | Comparison of repair index in cigarette and waterpipe smokers: A bio-monitoring assessment using human exfoliated buccal mucosa cells |
| title_full_unstemmed | Comparison of repair index in cigarette and waterpipe smokers: A bio-monitoring assessment using human exfoliated buccal mucosa cells |
| title_short | Comparison of repair index in cigarette and waterpipe smokers: A bio-monitoring assessment using human exfoliated buccal mucosa cells |
| title_sort | comparison of repair index in cigarette and waterpipe smokers a bio monitoring assessment using human exfoliated buccal mucosa cells |
| topic | micronucleus assays mouth mucosa smoking |
| url | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2022;volume=13;issue=1;spage=27;epage=27;aulast=Jalili |
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