Protection against neutrophil extracellular trap (NET) toxicity by antioxidant monoHER
Background: Neutrophil extracellular traps (NET) are extracellular fibers produced by activated neutrophils to kill bacteria. NET was recently found to be associated with several diseases, such as autoimmune diseases. NET formation, called NETosis, is reactive oxygen species (ROS) dependent, thereby...
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2019-08-01
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| Series: | Makara Journal of Health Research |
| Subjects: | |
| Online Access: | https://scholarhub.ui.ac.id/mjhr/vol23/iss2/8/ |
| _version_ | 1797893858689810432 |
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| author | Puji Astuti Danielle M A Beurskens Tanja Vajen Gerry A F Nicolaes Ming Zhang Guido R M M Haenen |
| author_facet | Puji Astuti Danielle M A Beurskens Tanja Vajen Gerry A F Nicolaes Ming Zhang Guido R M M Haenen |
| author_sort | Puji Astuti |
| collection | DOAJ |
| description | Background: Neutrophil extracellular traps (NET) are extracellular fibers produced by activated neutrophils to kill bacteria. NET was recently found to be associated with several diseases, such as autoimmune diseases. NET formation, called NETosis, is reactive oxygen species (ROS) dependent, thereby, prompting us to study its inhibition by potent antioxidant monoHER as well as to study monoHER protection against NET toxicity caused by NET constituent histone 3 on endothelial cells. Methods: Freshly isolated neutrophils from male donors were stimulated with PMA to induce NET formation. The effect of monoHER (50 µM) on oxidative burst (O2●− production) and NET formation was determined by fluorescence microscopy. Flow cytometry was used to determine the protective effect of monoHER against NET toxicity constituent histone 3 in EA.hy926 cells. Data was evaluated using ANOVA followed by the Bonferroni post-hoc test. Results: MonoHER significantly reduced (p < 0.01) O2●− production of PMA-stimulated neutrophils and consequently inhibited NET formation. MonoHER could also counteract histone 3 toxicity in EA.hy926 cells. Conclusions: MonoHER might inhibit ROS-dependent NETosis pathway and also protect the endothelial cells against NET toxicity. |
| first_indexed | 2024-04-10T06:59:39Z |
| format | Article |
| id | doaj.art-9774940bb64c434f81995fb2b25f9960 |
| institution | Directory Open Access Journal |
| issn | 2356-3664 2356-3656 |
| language | English |
| last_indexed | 2024-04-10T06:59:39Z |
| publishDate | 2019-08-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Journal of Health Research |
| spelling | doaj.art-9774940bb64c434f81995fb2b25f99602023-02-28T03:20:18ZengUniversitas IndonesiaMakara Journal of Health Research2356-36642356-36562019-08-0123210811510.7454/msk.v23i2.10453Protection against neutrophil extracellular trap (NET) toxicity by antioxidant monoHERPuji Astuti0Danielle M A Beurskens1Tanja Vajen2Gerry A F Nicolaes3Ming Zhang4Guido R M M Haenen5Department of Pharmacology and Toxicology, Faculty of Health, Medicine, and Life Science, Maastricht University, The NetherlandsDepartment of Biochemistry, Faculty of Health, Medicine, and Life Science, Maastricht University, The NetherlandsDepartment of Biochemistry, Faculty of Health, Medicine, and Life Science, Maastricht University, The NetherlandsDepartment of Biochemistry, Faculty of Health, Medicine, and Life Science, Maastricht University, The NetherlandsDepartment of Pharmacology and Toxicology, Faculty of Health, Medicine, and Life Science, Maastricht University, The NetherlandsDepartment of Pharmacology and Toxicology, Faculty of Health, Medicine, and Life Science, Maastricht University, The NetherlandsBackground: Neutrophil extracellular traps (NET) are extracellular fibers produced by activated neutrophils to kill bacteria. NET was recently found to be associated with several diseases, such as autoimmune diseases. NET formation, called NETosis, is reactive oxygen species (ROS) dependent, thereby, prompting us to study its inhibition by potent antioxidant monoHER as well as to study monoHER protection against NET toxicity caused by NET constituent histone 3 on endothelial cells. Methods: Freshly isolated neutrophils from male donors were stimulated with PMA to induce NET formation. The effect of monoHER (50 µM) on oxidative burst (O2●− production) and NET formation was determined by fluorescence microscopy. Flow cytometry was used to determine the protective effect of monoHER against NET toxicity constituent histone 3 in EA.hy926 cells. Data was evaluated using ANOVA followed by the Bonferroni post-hoc test. Results: MonoHER significantly reduced (p < 0.01) O2●− production of PMA-stimulated neutrophils and consequently inhibited NET formation. MonoHER could also counteract histone 3 toxicity in EA.hy926 cells. Conclusions: MonoHER might inhibit ROS-dependent NETosis pathway and also protect the endothelial cells against NET toxicity.https://scholarhub.ui.ac.id/mjhr/vol23/iss2/8/antioxidantsextracellular matrix proteinsflavonoidsmonoherneutrophilsprotective agents |
| spellingShingle | Puji Astuti Danielle M A Beurskens Tanja Vajen Gerry A F Nicolaes Ming Zhang Guido R M M Haenen Protection against neutrophil extracellular trap (NET) toxicity by antioxidant monoHER Makara Journal of Health Research antioxidants extracellular matrix proteins flavonoids monoher neutrophils protective agents |
| title | Protection against neutrophil extracellular trap (NET) toxicity by antioxidant monoHER |
| title_full | Protection against neutrophil extracellular trap (NET) toxicity by antioxidant monoHER |
| title_fullStr | Protection against neutrophil extracellular trap (NET) toxicity by antioxidant monoHER |
| title_full_unstemmed | Protection against neutrophil extracellular trap (NET) toxicity by antioxidant monoHER |
| title_short | Protection against neutrophil extracellular trap (NET) toxicity by antioxidant monoHER |
| title_sort | protection against neutrophil extracellular trap net toxicity by antioxidant monoher |
| topic | antioxidants extracellular matrix proteins flavonoids monoher neutrophils protective agents |
| url | https://scholarhub.ui.ac.id/mjhr/vol23/iss2/8/ |
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