Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma
BackgroundHepatocellular carcinoma (HCC) is an extremely malignant cancer with poor survival. H2AFY gene encodes for a variant of H2A histone, and it has been found to be dysregulated in various tumors. However, the clinical value, biological functions and correlations with immune infiltration of H2...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-11-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.723293/full |
| _version_ | 1818842200894603264 |
|---|---|
| author | Yongbiao Huang Shanshan Huang Li Ma Yali Wang Xi Wang Lingyan Xiao Wan Qin Long Li Xianglin Yuan |
| author_facet | Yongbiao Huang Shanshan Huang Li Ma Yali Wang Xi Wang Lingyan Xiao Wan Qin Long Li Xianglin Yuan |
| author_sort | Yongbiao Huang |
| collection | DOAJ |
| description | BackgroundHepatocellular carcinoma (HCC) is an extremely malignant cancer with poor survival. H2AFY gene encodes for a variant of H2A histone, and it has been found to be dysregulated in various tumors. However, the clinical value, biological functions and correlations with immune infiltration of H2AFY in HCC remain unclear.MethodsWe analyzed the expression and clinical significance of H2AFY in HCC using multiple databases, including Oncomine, HCCDB, TCGA, ICGC, and so on. The genetic alterations of H2AFY were analyzed by cBioPortal and COSMIC databases. Co-expression networks of H2AFY and its regulators were investigated by LinkedOmics. The correlations between H2AFY and tumor immune infiltration were explored using TIMER, TISIDB databases, and CIBERSORT method. Finally, H2AFY was knocked down with shRNA lentiviruses in HCC cell lines for functional assays in vitro.ResultsH2AFY expression was upregulated in the HCC tissues and cells. Kaplan–Meier and Cox regression analyses revealed that high H2AFY expression was an independent prognostic factor for poor survival in HCC patients. Functional network analysis indicated that H2AFY and its co-expressed genes regulates cell cycle, mitosis, spliceosome and chromatin assembly through pathways involving many cancer-related kinases and E2F family. Furthermore, we observed significant correlations between H2AFY expression and immune infiltration in HCC. H2AFY knockdown suppressed the cell proliferation and migration, promoted cycle arrest, and apoptosis of HCC cells in vitro.ConclusionOur study revealed that H2AFY is a potential biomarker for unfavorable prognosis and correlates with immune infiltration in HCC. |
| first_indexed | 2024-12-19T04:38:12Z |
| format | Article |
| id | doaj.art-977d0f56099c4dc1b0fb0e63b6cedd2a |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-19T04:38:12Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-977d0f56099c4dc1b0fb0e63b6cedd2a2022-12-21T20:35:42ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-11-011210.3389/fimmu.2021.723293723293Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular CarcinomaYongbiao HuangShanshan HuangLi MaYali WangXi WangLingyan XiaoWan QinLong LiXianglin YuanBackgroundHepatocellular carcinoma (HCC) is an extremely malignant cancer with poor survival. H2AFY gene encodes for a variant of H2A histone, and it has been found to be dysregulated in various tumors. However, the clinical value, biological functions and correlations with immune infiltration of H2AFY in HCC remain unclear.MethodsWe analyzed the expression and clinical significance of H2AFY in HCC using multiple databases, including Oncomine, HCCDB, TCGA, ICGC, and so on. The genetic alterations of H2AFY were analyzed by cBioPortal and COSMIC databases. Co-expression networks of H2AFY and its regulators were investigated by LinkedOmics. The correlations between H2AFY and tumor immune infiltration were explored using TIMER, TISIDB databases, and CIBERSORT method. Finally, H2AFY was knocked down with shRNA lentiviruses in HCC cell lines for functional assays in vitro.ResultsH2AFY expression was upregulated in the HCC tissues and cells. Kaplan–Meier and Cox regression analyses revealed that high H2AFY expression was an independent prognostic factor for poor survival in HCC patients. Functional network analysis indicated that H2AFY and its co-expressed genes regulates cell cycle, mitosis, spliceosome and chromatin assembly through pathways involving many cancer-related kinases and E2F family. Furthermore, we observed significant correlations between H2AFY expression and immune infiltration in HCC. H2AFY knockdown suppressed the cell proliferation and migration, promoted cycle arrest, and apoptosis of HCC cells in vitro.ConclusionOur study revealed that H2AFY is a potential biomarker for unfavorable prognosis and correlates with immune infiltration in HCC.https://www.frontiersin.org/articles/10.3389/fimmu.2021.723293/fullhepatocellular carcinomaH2AFYprognosisimmune infiltrationbiomarker |
| spellingShingle | Yongbiao Huang Shanshan Huang Li Ma Yali Wang Xi Wang Lingyan Xiao Wan Qin Long Li Xianglin Yuan Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma Frontiers in Immunology hepatocellular carcinoma H2AFY prognosis immune infiltration biomarker |
| title | Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma |
| title_full | Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma |
| title_fullStr | Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma |
| title_full_unstemmed | Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma |
| title_short | Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma |
| title_sort | exploring the prognostic value immune implication and biological function of h2afy gene in hepatocellular carcinoma |
| topic | hepatocellular carcinoma H2AFY prognosis immune infiltration biomarker |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.723293/full |
| work_keys_str_mv | AT yongbiaohuang exploringtheprognosticvalueimmuneimplicationandbiologicalfunctionofh2afygeneinhepatocellularcarcinoma AT shanshanhuang exploringtheprognosticvalueimmuneimplicationandbiologicalfunctionofh2afygeneinhepatocellularcarcinoma AT lima exploringtheprognosticvalueimmuneimplicationandbiologicalfunctionofh2afygeneinhepatocellularcarcinoma AT yaliwang exploringtheprognosticvalueimmuneimplicationandbiologicalfunctionofh2afygeneinhepatocellularcarcinoma AT xiwang exploringtheprognosticvalueimmuneimplicationandbiologicalfunctionofh2afygeneinhepatocellularcarcinoma AT lingyanxiao exploringtheprognosticvalueimmuneimplicationandbiologicalfunctionofh2afygeneinhepatocellularcarcinoma AT wanqin exploringtheprognosticvalueimmuneimplicationandbiologicalfunctionofh2afygeneinhepatocellularcarcinoma AT longli exploringtheprognosticvalueimmuneimplicationandbiologicalfunctionofh2afygeneinhepatocellularcarcinoma AT xianglinyuan exploringtheprognosticvalueimmuneimplicationandbiologicalfunctionofh2afygeneinhepatocellularcarcinoma |