T-cell-derived TNF-α and a cluster of immunological parameters from plasma allow a separation between SARS-CoV-2 convalescent versus vaccinated elite athletes
Guidelines for medical clearing after SARS-CoV-2 infection in elite athletes do not include T-cell immunity aspects despite its relevance in the course of COVID-19 disease. Therefore, we aimed to analyze T-cell-related cytokines before and after in-vitro activation of CD4+ T-cells. We sampled profes...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1203983/full |
| _version_ | 1827919168236683264 |
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| author | Jana Palmowski Sarah Kohnhorst Pascal Bauer Christian Puta Christian Puta Christian Puta Simon Haunhorst Kristina Gebhardt Thomas Reichel Christian Keller Magdalena Huber Hartmann Raifer Karsten Krüger |
| author_facet | Jana Palmowski Sarah Kohnhorst Pascal Bauer Christian Puta Christian Puta Christian Puta Simon Haunhorst Kristina Gebhardt Thomas Reichel Christian Keller Magdalena Huber Hartmann Raifer Karsten Krüger |
| author_sort | Jana Palmowski |
| collection | DOAJ |
| description | Guidelines for medical clearing after SARS-CoV-2 infection in elite athletes do not include T-cell immunity aspects despite its relevance in the course of COVID-19 disease. Therefore, we aimed to analyze T-cell-related cytokines before and after in-vitro activation of CD4+ T-cells. We sampled professional indoor sports athletes at medical clearing after SARS-CoV-2 infection obtaining clinical, fitness data, and serological data including CD4+ T-cell cytokines. All data were analyzed by principal component analysis and 2 × 2 repeated measures ANOVA. CD4+ T-cells were sampled for cell culture activation with anti-CD3/anti-CD28 tetramers. At medical clearing, CD4+ T-cells from convalescent athletes secreted increased levels of TNF-α 72 h after in-vitro activation compared to vaccinated athletes. IL-18 levels in plasma were elevated and a cluster of parameters differentiated convalescent from vaccinated athletes by 13 parameters at the timepoint of medical clearing. All clinical data indicate infection is resolved, while increased TNF-α may reflect altered proportions of peripheral T-cells as a hangover of infection. |
| first_indexed | 2024-03-13T03:54:00Z |
| format | Article |
| id | doaj.art-977d2ee5b76d4bab93bdbed290e7a044 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-03-13T03:54:00Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-977d2ee5b76d4bab93bdbed290e7a0442023-06-22T08:45:37ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-06-011410.3389/fphys.2023.12039831203983T-cell-derived TNF-α and a cluster of immunological parameters from plasma allow a separation between SARS-CoV-2 convalescent versus vaccinated elite athletesJana Palmowski0Sarah Kohnhorst1Pascal Bauer2Christian Puta3Christian Puta4Christian Puta5Simon Haunhorst6Kristina Gebhardt7Thomas Reichel8Christian Keller9Magdalena Huber10Hartmann Raifer11Karsten Krüger12Department of Exercise Physiology and Sports Therapy, Institute of Sports Science, Justus Liebig University Giessen, Giessen, GermanyCenter for Tumor and Immunology, Institute for Systems Immunology, Marburg, GermanyDepartment of Cardiology and Angiology, Justus-Liebig-University Giessen, Giessen, GermanyDepartment of Sports Medicine and Health Promotion, Friedrich Schiller University Jena, Jena, GermanyCenter for Sepsis Control and Care (CSCC), Jena University Hospital, Friedrich-Schiller-University Jena, Jena, GermanyCenter for Interdisciplinary Prevention of Diseases Related to Professional Activities, Jena, GermanyDepartment of Sports Medicine and Health Promotion, Friedrich Schiller University Jena, Jena, GermanyDepartment of Exercise Physiology and Sports Therapy, Institute of Sports Science, Justus Liebig University Giessen, Giessen, GermanyDepartment of Exercise Physiology and Sports Therapy, Institute of Sports Science, Justus Liebig University Giessen, Giessen, GermanyInstitute of Virology, Philipps University Marburg, Marburg, GermanyCenter for Tumor and Immunology, Institute for Systems Immunology, Marburg, GermanyCenter for Tumor and Immunology, Institute for Systems Immunology, Marburg, GermanyDepartment of Exercise Physiology and Sports Therapy, Institute of Sports Science, Justus Liebig University Giessen, Giessen, GermanyGuidelines for medical clearing after SARS-CoV-2 infection in elite athletes do not include T-cell immunity aspects despite its relevance in the course of COVID-19 disease. Therefore, we aimed to analyze T-cell-related cytokines before and after in-vitro activation of CD4+ T-cells. We sampled professional indoor sports athletes at medical clearing after SARS-CoV-2 infection obtaining clinical, fitness data, and serological data including CD4+ T-cell cytokines. All data were analyzed by principal component analysis and 2 × 2 repeated measures ANOVA. CD4+ T-cells were sampled for cell culture activation with anti-CD3/anti-CD28 tetramers. At medical clearing, CD4+ T-cells from convalescent athletes secreted increased levels of TNF-α 72 h after in-vitro activation compared to vaccinated athletes. IL-18 levels in plasma were elevated and a cluster of parameters differentiated convalescent from vaccinated athletes by 13 parameters at the timepoint of medical clearing. All clinical data indicate infection is resolved, while increased TNF-α may reflect altered proportions of peripheral T-cells as a hangover of infection.https://www.frontiersin.org/articles/10.3389/fphys.2023.1203983/fullT-lymphocyteSARS-CoV-2vaccinationconvalescenceelite athletes |
| spellingShingle | Jana Palmowski Sarah Kohnhorst Pascal Bauer Christian Puta Christian Puta Christian Puta Simon Haunhorst Kristina Gebhardt Thomas Reichel Christian Keller Magdalena Huber Hartmann Raifer Karsten Krüger T-cell-derived TNF-α and a cluster of immunological parameters from plasma allow a separation between SARS-CoV-2 convalescent versus vaccinated elite athletes Frontiers in Physiology T-lymphocyte SARS-CoV-2 vaccination convalescence elite athletes |
| title | T-cell-derived TNF-α and a cluster of immunological parameters from plasma allow a separation between SARS-CoV-2 convalescent versus vaccinated elite athletes |
| title_full | T-cell-derived TNF-α and a cluster of immunological parameters from plasma allow a separation between SARS-CoV-2 convalescent versus vaccinated elite athletes |
| title_fullStr | T-cell-derived TNF-α and a cluster of immunological parameters from plasma allow a separation between SARS-CoV-2 convalescent versus vaccinated elite athletes |
| title_full_unstemmed | T-cell-derived TNF-α and a cluster of immunological parameters from plasma allow a separation between SARS-CoV-2 convalescent versus vaccinated elite athletes |
| title_short | T-cell-derived TNF-α and a cluster of immunological parameters from plasma allow a separation between SARS-CoV-2 convalescent versus vaccinated elite athletes |
| title_sort | t cell derived tnf α and a cluster of immunological parameters from plasma allow a separation between sars cov 2 convalescent versus vaccinated elite athletes |
| topic | T-lymphocyte SARS-CoV-2 vaccination convalescence elite athletes |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1203983/full |
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