Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research
We generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDF) (MZT05) generated from a skin biopsy donated from a previously fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506121002555 |
| _version_ | 1818586091828019200 |
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| author | Erica C. Pandolfi Timothy J. Hunt Sierra Goldsmith Kellie Hurlbut Sherman J. Silber Amander T. Clark |
| author_facet | Erica C. Pandolfi Timothy J. Hunt Sierra Goldsmith Kellie Hurlbut Sherman J. Silber Amander T. Clark |
| author_sort | Erica C. Pandolfi |
| collection | DOAJ |
| description | We generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDF) (MZT05) generated from a skin biopsy donated from a previously fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent specifically for studying human fertility, infertility and germ cell differentiation. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal. Pluripotency was further verified using PluriTest analysis and in vitro differentiation was tested using Taqman Real-Time PCR assays. These sublines serve as controls for hiPSC research projects aimed at understanding the cell and molecular regulation of female fertility. |
| first_indexed | 2024-12-16T08:47:27Z |
| format | Article |
| id | doaj.art-977e1c71a6a94390aaeae5ff70535620 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-16T08:47:27Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-977e1c71a6a94390aaeae5ff705356202022-12-21T22:37:35ZengElsevierStem Cell Research1873-50612021-07-0154102409Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science researchErica C. Pandolfi0Timothy J. Hunt1Sierra Goldsmith2Kellie Hurlbut3Sherman J. Silber4Amander T. Clark5Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA USA; Molecular Biology Institute, University of California, Los Angeles, CA, USADepartment of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA USA; Molecular Biology Institute, University of California, Los Angeles, CA, USAInfertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USAInfertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USAInfertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USADepartment of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA; Corresponding author at: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA.We generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDF) (MZT05) generated from a skin biopsy donated from a previously fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent specifically for studying human fertility, infertility and germ cell differentiation. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal. Pluripotency was further verified using PluriTest analysis and in vitro differentiation was tested using Taqman Real-Time PCR assays. These sublines serve as controls for hiPSC research projects aimed at understanding the cell and molecular regulation of female fertility.http://www.sciencedirect.com/science/article/pii/S1873506121002555 |
| spellingShingle | Erica C. Pandolfi Timothy J. Hunt Sierra Goldsmith Kellie Hurlbut Sherman J. Silber Amander T. Clark Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research Stem Cell Research |
| title | Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research |
| title_full | Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research |
| title_fullStr | Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research |
| title_full_unstemmed | Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research |
| title_short | Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research |
| title_sort | generation of three human induced pluripotent stem cell sublines uclai005 a uclai005 b and uclai005 c for reproductive science research |
| url | http://www.sciencedirect.com/science/article/pii/S1873506121002555 |
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