Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens
Objective: This study aimed to isolate and investigate a bacterium from an Egyptian adult's healthy oral cavity, focusing on its probiotic properties, especially its antagonistic activity against oral pathogens. Methods: The isolated bacterium NT04 using 16S rRNA gene sequencing, was identified...
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| Language: | English |
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Elsevier
2023-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023034606 |
| _version_ | 1797814356348502016 |
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| author | Nashwa Tarek Ahmed F. Azmy Ahmed S. Khairalla Medhat Abdel-Fattah Ohoud A. Jefri Mohamed Shaban Abdelaziz A.A. El-Sayed Ahmed O. El-Gendy |
| author_facet | Nashwa Tarek Ahmed F. Azmy Ahmed S. Khairalla Medhat Abdel-Fattah Ohoud A. Jefri Mohamed Shaban Abdelaziz A.A. El-Sayed Ahmed O. El-Gendy |
| author_sort | Nashwa Tarek |
| collection | DOAJ |
| description | Objective: This study aimed to isolate and investigate a bacterium from an Egyptian adult's healthy oral cavity, focusing on its probiotic properties, especially its antagonistic activity against oral pathogens. Methods: The isolated bacterium NT04 using 16S rRNA gene sequencing, was identified as Enterococcus faecium. In this study, the whole genome of Enterococcus faecium NT04 was sequenced and annotated by bioinformatics analysis tools. Results: Numerous genes encoding the production of diverse metabolic and probiotic properties, such as bacteriocin-like inhibitory substances (Enterocin A and B), cofactors, antioxidants, and vitamins, were confirmed by genomic analysis. There were no pathogenicity islands or plasmid insertions found. This strain is virulent for host colonization rather than invasion. Conclusion: Genomic characteristics of strain NT04 support its potentiality as an anti-oral pathogen probiotic candidate. |
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| format | Article |
| id | doaj.art-977fbcd922334f58861c0fdd8b4f1e19 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T08:06:29Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-977fbcd922334f58861c0fdd8b4f1e192023-06-01T04:36:27ZengElsevierHeliyon2405-84402023-06-0196e16253Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogensNashwa Tarek0Ahmed F. Azmy1Ahmed S. Khairalla2Medhat Abdel-Fattah3Ohoud A. Jefri4Mohamed Shaban5Abdelaziz A.A. El-Sayed6Ahmed O. El-Gendy7Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Egypt; Basic Science Department, Faculty of Oral and Dental Medicine, Nahda University Beni-Suef (NUB), Beni Suef, EgyptMicrobiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, EgyptMicrobiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Egypt; Department of Biology, University of Regina, 3737 Wascana Parkway, Regina, SK, S4S 0A2, CanadaDepartment of Botany and Microbiology, Faculty of Science, Beni-Suef University, EgyptDepartment of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah, 21589, Saudi ArabiaDepartment of Physics, Faculty of Science, Islamic University of Madinah, Al-Madinah Al-Munawarah, 42351, Saudi Arabia; Nanophotonics and Applications (NPA) Lab, Physics Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514, EgyptBiology Department, Faculty of Science, Islamic University of Madinah, Al-Madinah Al- Munawarah, 42351, Saudi Arabia; Zoology Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt; Corresponding author. Zoology Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.Microbiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, EgyptObjective: This study aimed to isolate and investigate a bacterium from an Egyptian adult's healthy oral cavity, focusing on its probiotic properties, especially its antagonistic activity against oral pathogens. Methods: The isolated bacterium NT04 using 16S rRNA gene sequencing, was identified as Enterococcus faecium. In this study, the whole genome of Enterococcus faecium NT04 was sequenced and annotated by bioinformatics analysis tools. Results: Numerous genes encoding the production of diverse metabolic and probiotic properties, such as bacteriocin-like inhibitory substances (Enterocin A and B), cofactors, antioxidants, and vitamins, were confirmed by genomic analysis. There were no pathogenicity islands or plasmid insertions found. This strain is virulent for host colonization rather than invasion. Conclusion: Genomic characteristics of strain NT04 support its potentiality as an anti-oral pathogen probiotic candidate.http://www.sciencedirect.com/science/article/pii/S2405844023034606ProbioticsEnterococcus faeciumOral cavityWGS |
| spellingShingle | Nashwa Tarek Ahmed F. Azmy Ahmed S. Khairalla Medhat Abdel-Fattah Ohoud A. Jefri Mohamed Shaban Abdelaziz A.A. El-Sayed Ahmed O. El-Gendy Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens Heliyon Probiotics Enterococcus faecium Oral cavity WGS |
| title | Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens |
| title_full | Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens |
| title_fullStr | Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens |
| title_full_unstemmed | Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens |
| title_short | Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens |
| title_sort | genome sequencing of enterococcus faecium nt04 an oral microbiota revealed the production of enterocin a b active against oral pathogens |
| topic | Probiotics Enterococcus faecium Oral cavity WGS |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023034606 |
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