Expression of HMGB1 and NF-κB in Fetal Membranes of Premature Rupture of Membranes
Background: Inflammation, either sterile or infection-related, may lead to premature rupture of membranes (PROM). The non-histone nuclear proteins, high mobility group box-1 (HMGB1), and nuclear factor kappa-B (NF-κB) transcription factors have been extensively investigated in many disorders involvi...
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| Format: | Article |
| Language: | English |
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Universitas Padjadjaran
2023-12-01
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| Series: | Althea Medical Journal |
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| Online Access: | https://journal.fk.unpad.ac.id/index.php/amj/article/view/2911 |
| _version_ | 1797219473120296960 |
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| author | Ketut Edy Sudiarta Joan Khan Monicha Zalzabilla Aldinasyah Efyluk Garianto Retno Budiarti |
| author_facet | Ketut Edy Sudiarta Joan Khan Monicha Zalzabilla Aldinasyah Efyluk Garianto Retno Budiarti |
| author_sort | Ketut Edy Sudiarta |
| collection | DOAJ |
| description | Background: Inflammation, either sterile or infection-related, may lead to premature rupture of membranes (PROM). The non-histone nuclear proteins, high mobility group box-1 (HMGB1), and nuclear factor kappa-B (NF-κB) transcription factors have been extensively investigated in many disorders involving inflammatory reactions. This study aimed to determine the expression of HMGB1 and NF-κB in fetal membranes of PROM compared with non-PROM.
Methods: This study was an analytical observational study with a case-control design, performed from November 2021 to January 2022, including 40 fetal membrane samples (20 PROM and 20 non-PROM), which were obtained from pregnant women treated in the emergency unit of a hospital in Surabaya from August to November 2019 using the non-probability sampling method. The HMGB1 and NF-κB expressions were examined using the immunohistochemical method and further viewed under a light microscope (400x magnification), then assessed by Image-J software. The values were then compared between PROM and non-PROM, and analyzed using the Mann-Whitney U test.
Results: There was a significant difference (p<0.001) in the expression of HMGB1 and NF-κB in PROM and non-PROM for HMGB1 45.86± 14.21% vs. 8.50± 5.66% expression/mm2; and NF-κB 33.47±5.45% vs. 7.29±4.90% expression/mm2, respectively.
Conclusions: PROM groups have significantly higher expression of HMGB1 and NF-κB s, indicating their higher activity and contribution to PROM. |
| first_indexed | 2024-04-24T12:34:12Z |
| format | Article |
| id | doaj.art-9783afbc8886497f8ebf943792f2e3a2 |
| institution | Directory Open Access Journal |
| issn | 2337-4330 |
| language | English |
| last_indexed | 2024-04-24T12:34:12Z |
| publishDate | 2023-12-01 |
| publisher | Universitas Padjadjaran |
| record_format | Article |
| series | Althea Medical Journal |
| spelling | doaj.art-9783afbc8886497f8ebf943792f2e3a22024-04-07T14:07:19ZengUniversitas PadjadjaranAlthea Medical Journal2337-43302023-12-0110410.15850/amj.v10n4.29111656Expression of HMGB1 and NF-κB in Fetal Membranes of Premature Rupture of MembranesKetut Edy Sudiarta0Joan Khan1Monicha Zalzabilla Aldinasyah2Efyluk Garianto3Retno Budiarti4Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Hang Tuah Surabaya/Dr. Ramelan Central Naval Hospital SurabayaFaculty of Medicine, Universitas Hang Tuah SurabayaFaculty of Medicine, Universitas Hang Tuah SurabayaDepartment of Public Health Sciences, Faculty of Medicine, Universitas Hang Tuah Surabaya/Dr. Ramelan Central Naval Hospital SurabayaDepartment of Microbiology, Faculty of Medicine, Universitas Hang Tuah Surabaya/Dr. Ramelan Central Naval Hospital SurabayaBackground: Inflammation, either sterile or infection-related, may lead to premature rupture of membranes (PROM). The non-histone nuclear proteins, high mobility group box-1 (HMGB1), and nuclear factor kappa-B (NF-κB) transcription factors have been extensively investigated in many disorders involving inflammatory reactions. This study aimed to determine the expression of HMGB1 and NF-κB in fetal membranes of PROM compared with non-PROM. Methods: This study was an analytical observational study with a case-control design, performed from November 2021 to January 2022, including 40 fetal membrane samples (20 PROM and 20 non-PROM), which were obtained from pregnant women treated in the emergency unit of a hospital in Surabaya from August to November 2019 using the non-probability sampling method. The HMGB1 and NF-κB expressions were examined using the immunohistochemical method and further viewed under a light microscope (400x magnification), then assessed by Image-J software. The values were then compared between PROM and non-PROM, and analyzed using the Mann-Whitney U test. Results: There was a significant difference (p<0.001) in the expression of HMGB1 and NF-κB in PROM and non-PROM for HMGB1 45.86± 14.21% vs. 8.50± 5.66% expression/mm2; and NF-κB 33.47±5.45% vs. 7.29±4.90% expression/mm2, respectively. Conclusions: PROM groups have significantly higher expression of HMGB1 and NF-κB s, indicating their higher activity and contribution to PROM.https://journal.fk.unpad.ac.id/index.php/amj/article/view/2911fetal membranes, hmgb1, nf-κb, premature rupture of membranes |
| spellingShingle | Ketut Edy Sudiarta Joan Khan Monicha Zalzabilla Aldinasyah Efyluk Garianto Retno Budiarti Expression of HMGB1 and NF-κB in Fetal Membranes of Premature Rupture of Membranes Althea Medical Journal fetal membranes, hmgb1, nf-κb, premature rupture of membranes |
| title | Expression of HMGB1 and NF-κB in Fetal Membranes of Premature Rupture of Membranes |
| title_full | Expression of HMGB1 and NF-κB in Fetal Membranes of Premature Rupture of Membranes |
| title_fullStr | Expression of HMGB1 and NF-κB in Fetal Membranes of Premature Rupture of Membranes |
| title_full_unstemmed | Expression of HMGB1 and NF-κB in Fetal Membranes of Premature Rupture of Membranes |
| title_short | Expression of HMGB1 and NF-κB in Fetal Membranes of Premature Rupture of Membranes |
| title_sort | expression of hmgb1 and nf κb in fetal membranes of premature rupture of membranes |
| topic | fetal membranes, hmgb1, nf-κb, premature rupture of membranes |
| url | https://journal.fk.unpad.ac.id/index.php/amj/article/view/2911 |
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